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Citations for Thermo Fisher :
7501 - 7550 of 7737 citations for Recombinant Human FGFR4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Cell Biology 2021Quote: The whole genome siRNA screen was conducted as described (35) using HeLa S3 cells and the Dharmacon ON-TARGETplus® SMARTpool® human siRNA library (Thermo Scientific) and strain T3SA+.
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bioRxiv - Cancer Biology 2020Quote: ... human NSCLC cell lines and HARA human lung squamous carcinoma cell line were maintained in RPMI-1640 supplemented with 10% FBS (Gibco BRL, Gaithersburg, MD). Cell transfection was carried out by Lipofectamine 2000TM (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and after 24h of incubation fresh medium was added and cells were transfected with 100ng of total RNAs from cell cultures or human tissues using Lipofectamine 2000® (11668-019, Thermo Fisher Scientific). As positive controls ...
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bioRxiv - Cell Biology 2021Quote: Human epithelial colon adenocarcinoma, Caco2, cells (ACC 169, DSMZ, Leipzig, Germany) were grown in DMEM/F-12 (Gibco, Thermo Scientific, Waltham, Massachusetts, USA) supplemented with 10 % heat inactivated fetal bovine serum (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Immunology 2021Quote: ... Fresh human peripheral blood mononuclear cells were used as effector cells after red blood cell lysis with ACK lysing buffer (ThermoFisher Scientific, Waltham, MA). After 1 h incubation at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Post-selection iPSCs with stably-integrated human NGN2 under a tetracycline-inducible promoter were dissociated into single cells using Accutase (Thermo Scientific Cat. #A1110501) and plated on a Matrigel (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained for 30 min on ice using AlexaFluor700-conjugated anti-Human CD54/ICAM-1 (clone 1H4, Life Technologies, #MA528553, 1/75), PE-Cy7-conjugated anti-Human CD106/VCAM-1 (clone STA ...
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bioRxiv - Neuroscience 2021Quote: Lund human mesencephalic (LUHMES) neuronal precursors were grown in T75 flasks in proliferation medium (DMEM/F12 GlutaMAX™ supplement medium (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... MCF10A human breast epithelial cells (CRL-10317, ATCC, Manassas, VI, USA) were maintained in DMEM/F12 medium (11039-021, Invitrogen, Carlsbad, CA, USA) supplemented with 20 ng.ml-1 epidermal growth factor (AF-100-122 ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... All primer pairs were tested alone and in multiplexed PCR reactions using 10 ng of TaqMan® Control Human Genomic DNA (Thermo Fisher Scientific) in 10 μl reaction volumes ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... the VEGF-A amount in the conditioned medium of day 14 was measured using a VEGF-A Human ELISA Kit (Thermo Fisher Scientific, USA). The assay was performed according to the manufacturer’s protocol and absorbance was read at 450nm ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Cell Biology 2020Quote: ... human milk cells were isolated by diluting milk samples in an equal volume of sterile phosphate-buffered saline (PBS) (ThermoFisher Scientific, Waltham, U.S.) and centrifuged at 870 g for 20 minutes at 20°C in a Rotanta 460R centrifuge (Hettich ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Genetics 2021Quote: ... 250 000 cells in 2 ml of medium in a six-well plate were transfected using 12.5 pmol of Silencer Select siRNA against human ADCY9 (Ambion cat # 4390826 ID 1039), Silencer Select siRNA against CETP (Ambion cat 4392420 ID 2933 ...
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bioRxiv - Microbiology 2020Quote: ... Briefly, parasites were maintained in 2% human O+ erythrocytes (Interstate Blood Bank, Memphis, TN) in RPMI-1640 medium (Life Technologies, Grand Island, NY) supplemented with 0.5% Albumax II (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... 4 were used to measure the message levels of these human or mouse genes in cells or lung tissue (Life Technologies, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: ... The human embryonic kidney 293T/17 cells (ATCC, CRL-11268) were maintained in maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions prepared by enzyme-free CDS were kept in PBS-5% FCS and incubated on ice for 30 min with rabbit polyclonal anti-human HVEM (R140; 1:500) (Terry-Allison et al., 1998) followed by visualizing HVEM with anti-rabbit AF488 (Life Technologies; 1:200). The size of the epidermal samples mostly allowed only the analysis of nectin-1 expression ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... HTB-14) human glioblastoma cells were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F-12, ThermoFisher Scientific cat. no. 11330057) supplemented with 10% fetal bovine serum (FBS premium grade ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human MSCs (Cyagen Biosciences, Suzhou, China) from bone marrow were cultured in a minimum essential medium alpha (MEM-α, Gibco, Carlsbad, CA) medium with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Paraffin-embedded human lung tissue of control and IPF donors was cut with a Microm HM355S microtome (Thermo Fisher Scientific, Waltham, MA, USA) into serial sections of 3 μm ...
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bioRxiv - Immunology 2022Quote: ... Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific, Cat.11131D) at 1:1 ratio for two days ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Cancer Biology 2022Quote: Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A+B Card Set v3.0 (Applied Biosystems, Foster City, CA).
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bioRxiv - Neuroscience 2024Quote: ... The GFP plasmid and GPRC6A-Myc plasmid were transfected into the HEK293T that stably expresses a tetracycline-inducible wild-type human tau (4R0N) (iHEK-Tau) using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™, #11668019). Doxycycline was added to the cells to induce tau production on the first day of transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Human MPB CD34+ cells from a healthy volunteer were thawed using chilled Iscove’s Modified Dulbecco’s Medium (IMDM) (Thermo Fisher Scientific, Waltham, MA, USA,) supplemented with 2% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA of human TAF2 was amplified by PCR with gene-specific primers fused to attB recombination sequences for Gateway® cloning (Thermo Fisher Scientific). The amplified sequence was recombined via BP reaction into the pDONR201 donor resulting in pENTR-TAF2 for N-terminal tagging ...
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bioRxiv - Molecular Biology 2024Quote: ... crude and enriched synaptosomal preparations and surgically resected human brain samples was extracted using TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System (Invitrogen, cat. no. A33254). The remaining gDNA was removed using TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Cell Biology 2024Quote: Plasma TPO levels were estimated in plasma samples using Human PracartaPlex Mix&Match 4-Plex Luminex kit (ThermoFisher Scientific, Cat # PPX-04-MX323UK). Assays were performed as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent EnSC monolayers in 6-well plates were downregulated overnight in 2% DCC-FBS/DMEM/F12 media and transfected with 50nM Stealth siRNA against human GIPC1 or APPL1 (Fisher Scientific, Loughborough, U.K.). For depletion of receptor expression ...