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Citations for Thermo Fisher :
7351 - 7400 of 7737 citations for Recombinant Human FGFR4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293 cells (HEK 293) were maintained in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Waltham, Massachusetts) supplied with 10% fetal bovine serum ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Microbiology 2020Quote: ... All parasites obtained from other sources or generated in this study were maintained under standard conditions (37) at 2% hematocrit with O+ human erythrocytes in RPMI1640 (Thermo Fisher Scientific) containing hypoxanthine ...
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bioRxiv - Molecular Biology 2022Quote: Human K562 cells (ATCC CCCL-243) were grown at 37°C and 5% CO2 in RPMI 1640 medium +GlutaMAXTM(Gibco 61870-036) supplemented with 10% FBS (HyClone SH30071 ...
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bioRxiv - Cell Biology 2022Quote: ... media was collected at the end of 48 h of lipid loading and albumin concentration was measured with a commercially available human albumin ELISA kit (Invitrogen, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... restimulated as well as an unstimulated Mel15-PBMC sample from the same timepoint using the Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen). Single ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Immunology 2022Quote: ... 11D5-3 single chain variable fragment (scFv)3 and the human heavy-chain-only FHVH3316 were codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific). Retroviral constructs encoding APRIL CARs ...
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bioRxiv - Immunology 2023Quote: The coding sequence of wild-type human CXCR4 was synthesized by General Biol (Chuzhou, China) and was cloned into the pFastBac transfer plasmid (Invitrogen, Carlsbad, CA). Heat-stabilized BRIL ...
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bioRxiv - Cancer Biology 2024Quote: Human ccRCC cell lines 786-O and RCC4 were carried in DMEM/F12 (Cytiva #SH30023) + 10% fetal bovine serum (Gibco #16000-044) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Systems Biology 2022Quote: The human monocytic cell line THP-1 (LGC, ATCC-TIB-202) was cultured and expanded in RPMI 1640 media (Gibco, 61870-010) supplemented with 10% FCS (BioConcept ...
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bioRxiv - Molecular Biology 2022Quote: ... human embryonic kidney 293T cells were seeded at a density of 13×106 cells per 15cm plate in DMEM (Thermo Fisher, 11965092) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Bioengineering 2024Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS (cat# 16141079 ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 human colon carcinoma cells (ATCC, CCL-247) were maintained in Dulbecco’s Modified Eagle Media (DMEM) supplemented with GlutaMAX (Thermo Fisher Scientific #31966047), 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: ... Human neutrophil-like HL-60 cells were gifted by Orion Weiner (UCSF) and grown in supplemented RPMI medium 1640 (Gibco #22400-089) as described previously 28,54 ...
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bioRxiv - Cancer Biology 2024Quote: ... All human lung cancer cell lines were obtained from the ATCC and cultured in RPMI (Roswell Park Memorial Institute) 1640 (Gibco 11875-093) supplemented with 10%(v/v ...
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bioRxiv - Cancer Biology 2024Quote: Primary T-cells (CD4 and CD8; 1:1 ratio) were activated for 24 hours with Dynabeads™ Human T-Expander CD3/CD28 (Thermo Fisher) at 3:1 bead ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from these transfected cells and sent to the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet for Human Clariom™ S assay (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used (all at 1:100 dilution in immunofluorescence, 1:1000 in immunoblots): CX36 mouse anti-human (Invitrogen, clone 1E5H5), rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662) ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic®) and subcloned into pENTR (Life technologies®). GST- ...
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bioRxiv - Microbiology 2023Quote: Human pharyngeal Detroit 562 cells (ATCC CCL-138) were cultured in Gibco™ Minimum Essential Media (MEM) (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% non-heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from Illumina and the Axiom™ Genome-Wide Human Origins (HO) and the Axiom™ Precision Medicine Diversity Array Plus Kit (PMDA) from ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... of the human BPTF was amplified by PCR and subsequently cloned into the pDEST-566 vector using Gateway technology (Thermo Fisher Scientific). The resulting 6x-MBP-hBPTF (701-1073 ...
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bioRxiv - Immunology 2024Quote: ... washed in PBS/T and then incubated with 50 µL γ-chain specific goat anti-human IgG-alkaline phosphatase (AP) (Thermo Fisher) at a 1/2000 dilution for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2024Quote: ... Human A549 (mCherrypos and Luciferasepos) and HEK-293T tumor cells (in-house stocks) have been cultured in DMEM medium (Thermo Fisher 11995) supplemented like RPMI medium for T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse GBM cell lines and human prostate cancer cells were maintained in complete RPMI1640 (Media Preparation Core, Cleveland Clinic) supplemented with 10% FBS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Media Preparation Core ...
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bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... Human LRRK2 G2019S and LRRK2 Knock-out (KO) iPSC lines were generated in human iPSCs obtained from a female clone from Thermo Fisher (#A18945). Sequence information for generating targeting gRNA ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... and KYSE180 (NRF2D77V) and NRF2low KYSE450 (NRF2WT) cells (human ESCC-DSMZ, Braunschweig, Germany) 20 were cultured in Gibco RPMI1640 GlutaMAX (ThermoFisher, Waltham, MA) supplemented with 10% FBS and 1% antibiotics (penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37◦ C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... at the final concentration of 5 µM and one of the following anti-human mouse monoclonal antibodies (5 µl per sample) conjugated with allophycocyanin (APC): anti-CD9 (clone MEM-61, Thermo Fisher Scientific), anti-CD63 (clone MEM-259 ...