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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Protein A magnetic Dynabeads (80 uL, Invitrogen) were washed with 1% BSA/PBS before adding to the antibody-bound chromatin lysates and incubated overnight at 4°C with rotation ...
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bioRxiv - Biophysics 2024Quote: ... followed by a laminin mouse protein (Gibco) layer at 10 μg/mL ...
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bioRxiv - Genetics 2024Quote: ... antibodies coated to Protein A Dynabeads (Invitrogen). Real-time quantitative PCR was performed using iTaq universal SYBR Green (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... proteins were detected using Femto (ThermoFisher Scientific) and a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... in conjunction with Dynabeads Protein-G (Invitrogen), following previously established protocol (Maddala et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Strep-tagged Nucleocapsid protein (Invitrogen, MA5-37747), phospho-Glycogen Synthase (Cell Signaling ...
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bioRxiv - Biochemistry 2024Quote: ... or protein G dynabeads (10004D, Thermo Fisher) pre-bound to the indicated antibodies ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... PageRuler Prestained protein ladder (#26616; ThermoFisher Scientific) was used as a molecular weight marker ...
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bioRxiv - Cell Biology 2024Quote: ... Halt Complete protein inhibitor (Thermo Scientific, 78429)) per sample and harvesting by rapid pipetting of ibidi flow slides with a 1 mL syringe with Luer-Lok (Fischer scientific ...
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bioRxiv - Microbiology 2024Quote: ... protein expression from pBAD-His/B (Invitrogen) derivative plasmids was induced at the temperature shift by adding 0.01–0.2% L-arabinose ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant protein G sepharose beads (101242, Invitrogen), RNase T1 (EN0541 ...
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bioRxiv - Microbiology 2024Quote: ... proteins were visualised (ChemidocTM, ThermoFisher Scientific, USA) by enhanced chemiluminescent (ECL ...
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bioRxiv - Microbiology 2024Quote: ... 50uL of Protein A Dynabeads (Life Technologies) was prepared by washing twice in RIPA before incubating with 15uL of anti-RIG-I rabbit serum (969 ...
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bioRxiv - Molecular Biology 2024Quote: ... protein G magnetic beads (Thermo Fisher, 88847) were added for an additional 1 hour at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Bis-Tris protein gels (Thermo Fisher, NP0321BOX) followed by electrophoretic transfer to nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... stained with Imperial protein stain (ThermoFisher Scientific) for 2 hours at room temperature with shaking and destained overnight with mqH20 at room temperature with shaking ...
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bioRxiv - Plant Biology 2024Quote: ... PageRuler™ Plus prestained protein ladder (ThermoFisher) was used for reference of protein size ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein residue level was below the minimum detection threshold of Micro BCA Protein Assay Kit (Thermo Fisher, Cat. # 23235), and no bacterial colonies formed in bioburden detection.
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was taken and total protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 35 μl of extract (about 4 mg/ml protein ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of extracted protein was measured using Pierce 660 nm Protein Assay Reagent (Thermo Fisher, Cat No. 1861426) and Bio-Rad SmartSpecTM 3000 spectrophotometer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the cell and tissue lysates were determined using BCA protein assay kit (Pierce, Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE and proteins were transferred onto a nitrocellulose membrane (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Following determination of the protein concentration in the supernatants (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific, 23250), samples were prepared for denaturing gel electrophoresis by addition of Laemmli Sample Buffer (BioRad ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were quantified using 660 nm Protein Assay Reagent with Ionic Detergent Compatibility Reagent (IDCR) (Thermo Fisher Scientific). Equal amounts of protein extracts underwent vacuum filtration through a pre-wet 0.2 μm pore size nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the RIPA-soluble fraction was measured using a BCA protein assay kit (Thermo Fisher Scientific). The protein concentration of the urea-soluble fraction was assumed to have the same ratio as the RIPA-soluble fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the sample was incubated with 300 µg protein A or protein G dynabeads (Life technologies, Cat 10001D or 10003D) for 2hrs with rotation at 4℃ ...
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bioRxiv - Physiology 2022Quote: ... The membrane was subsequently stained for total protein with Pierce™ Reversible Protein Stain Kit (Thermo Fisher, Waltham, MA). The membranes were probed with Anti-O-Linked N-Acetylglucosamine antibody (anti-RL2 ...
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bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
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bioRxiv - Physiology 2022Quote: ... The total protein concentration in the lysates was determined using a Pierce BCA Protein Assay kit (Thermo Scientific, 23225). A 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels was used ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, catalog #23227) and Gen5 3.04 software (BioTek) ...
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bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was evaluated and 1 mg total protein was incubated with 30 μl streptavidin-magnetic beads (ThermoFisher, 88816) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The total amount of protein was quantitated by using Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal amounts of proteins were resolved on 10 or 15% Tris-glycine SDS-PAGE under reducing conditions and transferred onto Immobilon-P Membrane ...
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bioRxiv - Cell Biology 2022Quote: Total protein was quantified for all samples using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific 23225) as per the manufacturer’s instructions.
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Genomics 2020Quote: ... Total protein present in the supernatants were quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Relative amounts of toxin were normalized to total proteins.
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bioRxiv - Cancer Biology 2020Quote: ... Supernatants were harvested and the protein concentration was measured using the Pierce BCA protein assay kit (ThermoFisher Scientific, 23225). Samples were mixed with NuPAGE LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... The identified proteins were filtered using the high peptide confidence setting in Protein Discoverer (version 2.2, Thermo Fisher Scientific), which correspond to a filter for 1% FDR on peptide level ...
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bioRxiv - Bioengineering 2019Quote: ... Protein concentration for each sample was estimated in a single technical replicate using the Qubit Protein Assay Kit (Invitrogen) and the Qubit 3.0 Fluorometer (Invitrogen).
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bioRxiv - Cancer Biology 2019Quote: ... followed by incubation with 7.5 µL each of Protein A- and Protein G-Dynabeads (Life Technologies 10001D and 10003D) at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was saved and protein concentration was quantified using Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA). Protein samples were denatured for 5 min ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates were prepared in either RIPA or CHAPS buffer and quantified using the BCA Protein Assay (Thermo Scientific). Protein was separated on 4%–20% SDS-PAGE gels ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations of whole-cell lysate fractions were then determined using the BCA Protein Assay Reagent kit (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... The amount of proteins of each sample was measured with Pierce BCA protein Assay Kit (Thermo Fisher, Waltham, MA) and normalized to the smaller concentration obtened ...
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bioRxiv - Immunology 2021Quote: ... Samples were quickly spun down and protein concentration in the supernatants was quantified by BCA protein assay (Thermo scientific). Co-cultures were performed by adding T cell clones that had rested overnight in fresh media without IL-2 to wells containing DCs ...
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bioRxiv - Genetics 2019Quote: ... Whole protein concentration was measured using Pierce BCA Protein Assay Kit according to the manufacturer’s protocol (Thermo Fisher Scientific). SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... The protein concentration of each sample was analyzed using a Pierce™ BCA Protein Assay Kit (Thermo Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension was incubated with preequilibrated protein A/G magnetic beads (Thermo Fisher Scientific; 20 μl slurry/mg protein) for 1 hour at 4°C with gentle shaking ...