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Citations for Thermo Fisher :
701 - 750 of 6994 citations for PRTN3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were co-transfected with 200 ng of GeneArt CRISPR Nuclease mRNA (Invitrogen #A29378) in addition to sgRNA prepared by mixing fluorescently labelled Alt-R CRISPR-Cas9 tracrRNA ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293/CRE-Luc/hCXCR4 cells were plated in Opti-MEM media (Invitrogen, cat# 31985-070) supplemented with 0.1% FBS (Seradigm ...
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bioRxiv - Bioengineering 2024Quote: ... All other CYpHER molecules were produced by transient expression in suspension HEK293 cells (ThermoFisher GeneArt) and purified either via Ni-NTA pull-down as previously described (33 ...
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bioRxiv - Bioengineering 2024Quote: ... pH change experiment for oROS-HT-C199S were performed with HEK293s in PBS (10010001, Gibco) prepared at pH of 6 ...
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bioRxiv - Biophysics 2023Quote: ... The construct was transfected in HEK293 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA #11668019), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1 ...
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bioRxiv - Genetics 2023Quote: HEK293 cell protein was extracted with 0.3 mL of RIPA buffer (ThermoFisher, Cat. No. 89900) containing Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in high-glucose (4.5 g/l) DMEM (Thermo Fisher) for BRET assays ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI- cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific, cat# 21969035) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... 1 mln HEK293 Flp-In T-REx cells were grown on DMEM High glucose (Gibco) supplemented with 10% fetal bovine serum on 6-well plates ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were plated at 50% confluency overnight and transfected using Lipofectamine 3000 kit (ThermoFisher) with ∼0.5 μg of CPEB3 DNA ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293 cell lines were maintained in phenol red-free DMEM media (Gibco, Waltham, MA) supplemented with 2 mM GlutaMAX (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and RAW 264.7 cells were obtained from ATCC and were cultured in DMEM (Gibco) supplemented with 10% FBS (Wisent ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 and P19 cell lines were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, C11995500BT) supplementary with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were transfected with appropriate plasmids using either Lipofectamine 3000 (Thermo Fisher Scientific Inc.) or JetPrime (Polyplus Transfection Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were incubated for 1.5 hours with glutamate-free DMEM GlutaMAX-I (Life Technologies), to reduce the extracellular concentration of glutamate ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s serum (DMEM) (Thermo Fisher Scientific, Oberhausen, Germany) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The HEK293/Flp-In/T-Rex were additionally cultured with 1x penicillin/streptomycin (ThermoFisher 10378016). To begin experiments ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen). The cells were replated 2 days after transfection and treated with 1 mg/mL G418 sulfate (EMD Chemicals Inc. ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells (CRL-1573, ATCC, female) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: SH-SY5Y and HEK293/T cells were transfected with siRNA using Lipofectamine 3000 (Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were tested for efficacy in HEK293 (ATCC) using a genomic cleavage kit (GeneArt, ThermoFisher). The best sgRNA was used for editing and had the sequence ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 cells were transiently transfected following the reverse transfection method using LipofectamineTM 3000 (Thermo Fisher). Two transfection mixes (A & B ...
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bioRxiv - Genetics 2024Quote: ... Transfections were performed in HEK293 cells using Lipofectamine™ 3000 Transfection Reagent (L3000015, Thermo Fisher) and serum-free Opti-MEM I medium (31985 ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into pcDNA3.1D/V5-His-TOPO (Thermo Fisher Scientific). MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS) (Gibco) at 37°C in the presence of 5% carbon dioxide (CO2) ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... for over-expression or pcDNA4/TO/myc-his-B (Thermofisher #V103020 ...
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 400 nM FITC-labeled TRAP220 or NCoR peptide in a buffer consisting of 20mM potassium phosphate (pH 8) ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 5 nM Fluormone Pan-PPAR Green fluorescent tracer ligand (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...
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bioRxiv - Cell Biology 2021Quote: ... A 1:10,000 dilution of mouse α-(6X)His (Invitrogen) primary antibody in PBST/3% BSA was applied for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in 12μl of Hi-Di formamide (Thermo fisher, 4311320), denatured at 95°C for 5 mins followed by snapchill ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) or Herculase II Fusion (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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bioRxiv - Immunology 2020Quote: ... 1 mg of magnetic bead (Dynabeads™ His-Tag, Invitrogen) were coated with 70 μg of histidine tagged RBD ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... secondary anti-6x-His tag monoclonal antibody (Invitrogen, # MA1-21315) at 1:175 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.