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Citations for Thermo Fisher :
601 - 650 of 6562 citations for PRTN3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Transfected HEK293 cells were fixed with 10% neutral buffered formalin (Fisher Scientific SF100-4) for 30 minutes then blocked and permeabilized for 30 minutes with 10% goat serum (Sigma S26-LITER) ...
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bioRxiv - Cell Biology 2020Quote: All plasmid transfections to Huh7 and HEK293 cells were done with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Gemini Bio-products ...
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bioRxiv - Neuroscience 2021Quote: HEK293 and C6 Glioblastoma cells were grown in Dulbecco’s modified Eagles medium (DMEM,Gibco) supplemented with 2mM L-glutamine,10% heat inactivated fetal bovine serum (FBS).
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bioRxiv - Cell Biology 2019Quote: HEK293 (CRL-1573, ATCC, female)were cultured in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Biophysics 2021Quote: HEK293 cells were electroporated with plasmids using the Neon 100 Kit (Invitrogen, Carlsbad, USA) and a MicroPorator (Digital Bio ...
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bioRxiv - Microbiology 2020Quote: HEK293 and A549 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco-BRL) containing 4 mM glutamine and 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Transient transfections were performed with either lipofectamine 2000 reagent from Invitrogen (Hek293 cells, MDCK) or with FuGENE 6 (ProMega ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells (CRL-1573, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (#11965126, Gibco) containing 10% fetal bovine serum (12306C ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells (ATCC CRL-1573) were grown in Dulbecco modified Eagle medium (DMEM, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS).
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bioRxiv - Molecular Biology 2023Quote: HEK293T or HEK293-JumpIN cells were cultured with Gibco DMEM with Glutamax (Life Technologies) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells and A549 cells were cultured in DMEM media (Thermo Fisher #11965-092) containing 1% Pen/Strep and 10% FBS (VWR International ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (ATCC) and were all grown in 1x DMEM based media (ThermoFisher Scientific) with 1% Penicillin and Streptomycin (Pen/Strep ...
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bioRxiv - Molecular Biology 2023Quote: ... and HEK293 cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; TM4, HeLa: Gibco™ #41966 – high glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... The Flp-In HEK293 cells were cultured in high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... the transiently transfected HEK293 cells were harvested using dissociation buffer TrypLE (Thermo Fisher Scientific) and binding assay buffer ...
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bioRxiv - Bioengineering 2023Quote: The plasmid was transfected into HEK293 cells using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were cultured in DMEM/F-12 cell culture medium (Gibco, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells were cultured in Freestyle™ 293 expression medium (Gibco).
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bioRxiv - Biochemistry 2023Quote: HEK293 and C6 glioblastoma cells were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal calf serum (HI-FCS ...
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bioRxiv - Neuroscience 2023Quote: ... The Flp-In T-REx HEK293 cell line was used (Thermo Fisher Scientific, R78007). Stably expressing cells were isolated by treating with 200 µg/mL hygromycin B (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transfection was used to transiently transfect HEK293 cells using Lipofectamine 3000 (Thermo Fisher), using the method provided by the manufacturer ...
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Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... fixed HEK293 RyR2D4365-GFP cells were incubated with MA3-916 RyR2 monoclonal antibody (Invitrogen) diluted 1:100 at 4°C overnight in the same solution as above ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transfected into HEK293-T or LNCaP cells using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples generated from HEK293 cells were analyzed with an LTQ-Orbitrap Velos (Thermo Fisher) while samples from HeLa cells with a Q Exactive (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: 5×105 HEK293 cells were plated on a 35 mm cell culture dish (Nunc) one day before transfection ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected with plasmids using 293fectinTM Transfection Reagent (Cat: 12347019, Life Technologies) when the cell density reached 1 × 106 cells/mL ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Developmental Biology 2021Quote: HEK293 cells were transfected with myc or myc-MLLT11 vectors using Lipofectamine™ 2000 (Invitrogen). 24 hours later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK293 were performed with 1.0 µg of expression vector using Lipofectamine 2000 (ThermoFisher). After 12 to 30 h of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... U2OS and HEK293 cells were purchased from ATCC cell repository and cultured in DMEM (Gibco), supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 and SH-SY5Y cells were transfected with 25 nM siSARM (s23032, Ambion, life technologies) for 48 h followed by 24 h rotenone treatment ...
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bioRxiv - Genetics 2019Quote: HEK293 cells used for indel acquisition assays were transfected with Liopofectamine 3000 (Thermo Fisher #L3000008) according to manufacturer’s protocol with 5ug of ErCas12a plasmid targeting each site ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM L-glutamine) and used to infect 80-100 % confluent HEK293-TRex cells (ThermoFisher) grown in 96-well plates (BD Purecoat Amine ...
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bioRxiv - Immunology 2019Quote: ... HEK293 T cells were transfected in 12-well plates with Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... For expression in the HEK293 derived Expi293F™ cells (Thermo-Fisher Scientific, Cat. No. A14527), full-length ZMPSTE24 gene was cloned into the pHTBV1.1-LIC baculovirus transfer vector (The BacMam vector backbone ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 cell line were seeded and treated with Lipofectamine 3000 (Thermo Scientific, Waltham, MA, USA) and 1µg/ml DNA in OptiMEM reduced-serum medium (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with FLAG-tagged OGA using Lipofectamine 3000 reagent (Thermo Fisher Scientific) at a ratio 1:2 (μg:μl ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells and Mouse Embryonic Fibroblasts (MEFs) were grown in DMEM (11965-092, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 and HeLa cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) with 10% heat-inactivated FBS (BioInd) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were plated in 6-well plates and maintained in high glucose DMEM (Invitrogen) growth media supplemented with 1% pen/strep (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 10% (HEK293) or 12% (C17.2 or SHSY5Y) of newborn calf serum (Thermo Fisher Scientific). Endogenous TH were depleted from the serum by stripping with activated charcoal (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells (1×105) were transfected into HEK293 with 1µg plasmid DNA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... removed supernatant and resuspended cells in serum free medium HEK293-SFMII (Gibco, catalog #: 11686-029), supplemented with 25 mM HEPES (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were plated in a 15-cm dish in 25ml of DMEM/F12 (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Mouse Embryonic Fibroblast (MEFs) and HEK293 (ATCC) cells were maintained in the DMEM medium (Gibco) containing glucose 1 g/L ...
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bioRxiv - Molecular Biology 2019Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (10566-016, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...