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Citations for Thermo Fisher :
701 - 750 of 1551 citations for Echovirus Antigen Recombinant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Immunology 2021Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 20 minutes at room temperature followed by 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set overnight and stained with antibodies against intracellular antigens (1 µg/ml) in Permeabilization buffer (ThermoFisher). For biotinylated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Immunology 2020Quote: ... Phage display selections were carried out by alternating between capture of the antigen on neutravidin coated wells on Maxisorp plates (Nunc) and streptavidin magnetic beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Immunology 2020Quote: ... surface antigen-labeled cells were fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and incubated with the fluorochrome-labeled antibodies listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2020Quote: Serum antibodies were purified from 25mL of remaining serum of final bleeds using antigen conjugated to NHS-activated agarose resin (ThermoFisher). Columns were washed three times with 1X PBS buffer ...
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bioRxiv - Immunology 2020Quote: To address neutrophils activation and phagocytic capacities we used pHrodo-labeled BioParticles and coated with Staphylococcus aureus (S. aureus) or Zymosan antigens (Invitrogen). pH-sensitive pHrodo dyes are useful for visualizing acidification process during phago-lysosome formation (29) ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were stained for extracellular antigens before EdU detection with click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were injected sub-cutaneously with 10 μg of fluorescent antigen in 100 μL mixed 1:1 with Imject Alum Adjuvant (ThermoFisher) in the upper and lower flank to target the brachial ...
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bioRxiv - Immunology 2022Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... by RT-PCR (see methods below) and (ii) by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42 ...
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bioRxiv - Developmental Biology 2023Quote: ... de-paraffinized and re-hydrated serial sections were subjected to antigen retrieval using 1x PT module buffer 1 (Thermo Scientific) for 36 min at 93°C using a KOS microwave Histostation (Milestone) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Antigen retrieval was performed by heating the slides in 10 mM sodium citrate buffer pH 6.0 (Thermo Fisher, S279-3) for 4 min in a microwave and left to cool down for 10 min at room temperature ...