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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... tissues were incubated with a species-specific secondary antibody (Alexa 647) for 1h then washed 3 times with PBS and mounted using ProLong Gold Antifade Mountant with DAPI (Invitrogen).
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bioRxiv - Developmental Biology 2020Quote: ... Dissociated murine prostate cells were obtained by digesting pre-minced prostate tissue for 1h at 37°C in digestive medium prepared in prepared in ADMEM/F12 (Gibco), and containing 1mg/mL Collagenase Type I (ThermoFischer Scientific ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells were grown on glass coverslips and incubated for 1h (37°C, 5% CO2) with EU from the Click-iT RNA Alexa Fluor 594 imaging kit (C10330, Invitrogen). Cells were fixed with 3,7% formaldehyde in PBS 1× for 15min at room temperature and permeabilized with 0.5% Triton X-100 in PBS 1× for 15min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... W4021) were added for 1h and protein signals were detected using Pierce™ ECL Western Blotting Substrate kit (ThermoFisher 32109), using a Fujifilm LAS4000 luminescence imager and band intensities quantified using ImageJ ...
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bioRxiv - Biochemistry 2020Quote: ... slides were rinsed with PBS and incubated for 1h with secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen) and DAPI (4083 ...
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bioRxiv - Microbiology 2021Quote: ... Following another 3 washes with PBS plates were incubated for 1h with secondary goat anti-guinea pig-AlexaFluor488 (Invitrogen, UK) at 1:500 in PBS-0.5%BSA ...
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bioRxiv - Microbiology 2020Quote: ... The non-encapsidated DNA was then removed by a 1h incubation at 37°C with 4 U TURBO DNase enzyme (Invitrogen), 1X TURBO DNase Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... washed thrice with PBST followed by 1h incubation with Alexa fluor conjugated secondary antibody (Thermo Fisher Scientific Cat # 11008, 21244) then again washed thrice with 1XPBST ...
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bioRxiv - Immunology 2022Quote: ... were generated by plastic adherence for 1h in complete RPMI 1640 (680 μM L-Glutamine and 10 mM Hepes, GIBCO) supplemented with 5% pooled human serum (The Blood Bank ...
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bioRxiv - Cell Biology 2021Quote: ... samples were incubated at room temperature for 1h with the corresponding fluorescence secondary antibodies conjugated to Alexa Fluor 488 or 555 (Invitrogen) at 1:400 ...
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bioRxiv - Bioengineering 2021Quote: ... Chips were then incubated at 37°C for ~1h until cells were fully dissociated into single cells at which point DTI (Gibco) was added to neutralize the enzymatic activity ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... were coated for 1h with 0.1% gelatin solution (ATCC, PCS-999-027) and washed twice with PBS (pH 7.4, Thermo Fisher Scientific). HEK293FT cells were plated on the gelatin coated dish and grown overnight in normal growth medium to reach ∼ 70% confluency ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were put back on ice and treated for 1h with anti-Alexa fluor® 488 (#A11094 from Life Technologies) in order to quench remaining surface signal ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Biophysics 2020Quote: ... fixed cells are incubated 1h at room temperature with Myosin 4 Monoclonal Antibody conjugated with Alexa Fluor 488 (MF20, Thermofisher) at 5μg/mL (1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualisation of the signal was conducted using Immobilon™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2023Quote: ... 100 uL ofmedium from the bottom chamber were collected at either 1h or 4h after Dextran addiction and fluorescence was quantified (Emission; 490, Absorption; 520) on black 96 multiwell plates (Nunc) on CLARIOstar OPTIMA (BMG LabTech ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated for 1h at room temperature in PBS- tween with 2.5% BSA and developed using ECL Western blot reagent (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were blocked in blocking buffer for 1h at room temperature and incubated with BODIPY 558/568 (Invitrogen, D38D35) for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Biophysics 2022Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... Blocking was 1h in PBS + 5% w/v bovine serum albumin (BSA) and 2.5 µg/mL anti-CD16-CD32 (FcBlock, Thermo Fisher). Primary antibodies (Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were incubated for 1h at 37°C with 5% CO2 and the cells were overlaid with 1X MEM (Gibco) containing 2% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 1h at room temperature in 3% BSA/PBS supplemented with 0.1% Tween 20 (BP337-100, Fisher Scientific), incubated at 4°C overnight with primary antibody in 3% BSA/PBS + 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 1h at RT with secondary antibody (4 µg/ml goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific) in PBS-BSA ...
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bioRxiv - Neuroscience 2020Quote: ... serum-free medium (DMEM:F12 at 2:1) supplemented with N2 (1% v/v; Invitrogen), 1mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated cortical neurons were infected with Control and Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Biochemistry 2024Quote: ... Solutions were further diluted to 1 µM using 2:1 MeOH:CHCl3 (ThermoFisher Scientific, Australia) with 5 mM ammonium acetate (SigmaAldrich ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 2% B27 and 1% L-glutamine and 1% Pen-Strep (Life Technologies). Dissociated wildtype and Ncan KO cortical neurons were initially infected with Control and the two Ncan shRNA AAVs at 7 days in vitro (DIV ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa647 ICAM-2 at 1:100 and Live/Dead at 1:1000 (L34962, Invitrogen), for sorting of CD45neg ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1% pen/strep and 1% (v/v) L-glutamine (2 mM final concentration, Gibco). Cells were maintained at 37°C and 5% CO2.
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bioRxiv - Biochemistry 2024Quote: ... 1 mM 2-mercaptoethanol and 1 mM PMSF) by sonication (using a Fisher Scientific Sonic Dismembrator with a Qsonica Ultrasonic Sonicator converter ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 2:1:1 ratio using Lipofectamine 3000 transfection reagents (Invitrogen, Cat. L3000015). Viral supernatants were collected 48 hours after transfection and filtered through a 0.45-μm membrane (Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were selected with 1 μg ml-1 and 2 μg ml-1 puromycin (Thermo Fisher Scientific) respectively for 10 days.
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected with 1 µg mL-1 and 2 µg mL-1 puromycin (Thermo Fisher Scientific), respectively.
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... PCs and ACs were stained with neural/glial antigen 2 (NG-2) antibody (eBioscience, 14-6504-80, 1:100) and glial fibrillary acidic protein (GFAP) antibody (Invitrogen, PA1-10019, 1:1000), respectively ...
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bioRxiv - Genetics 2024Quote: ... 2 µL of a 1:100 dilution of ERCC ExFold RNA Spike-In Mix 1 or Mix 2 (Thermo Fisher Scientific, #44-567-39) were added to 1 µg of total RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) staining for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 2°: goat anti-mouse AF488 (1:500) (Invitrogen, A-11001), and donkey anti-rabbit Cy5 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and ethidium homodimer-1 (2 mM in DMSO, L3224, ThermoFisher) were added directly to the cell medium to a final concentration of 1 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... stained with Neurotrace for 2 hours (1:500, N21479; Invitrogen), rinsed twice with 2x SSCT and mounted on a slide with Fluoromount-G ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Life Technologies) was added to visualize cell nuclei ...
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bioRxiv - Molecular Biology 2020Quote: ... and HA tag (2-2.2.14; ThermoFisher Scientific 26183, 1:10,000), used with goat anti-mouse IgG AlexaFluor®568 (ThermoFisher Scientific A-11004 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were loaded with 1 μM fura-2 AM (Invitrogen) for 40 min before the measurements at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), and 1× MEM non-essential amino acids solution (Gibco) ...