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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at RT with secondary antibodies (Alexa Fluor conjugated produced in goat by Thermo Fisher) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Prior to all measurements cells were starved in FCS and phenol red free medium for 1h (Gibco, 31053028). For sialidase treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 x 106 cells mixed 1:1 with Matrigel (Fisher Scientific, 356237) and were subcutaneously implanted into both flanks of the mice ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:1000 2-Mercaptoethanol (ThermoScientific, 21985023) and 1:1000 Gentamicin (Gibco, 1570060). After collection ...
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bioRxiv - Immunology 2022Quote: ... Whole blood samples were diluted 1:1 with PBS+2%FBS (Gibco) and subjected to Ficoll (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... an internal solution containing 1 mM Fura-2 (K5-Fura-2, cell impermeable, Invitrogen F1200), 140 mM KCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... Selection for 2 weeks was performed using puromycin (2 µg mL-1; Gibco, Themofisher Scientific). Cells were passaged at least 3 times before further experiments.
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked in 2% goat serum + 2% Bovine Serum Albumin for 2 hours at room temperature before primary antibody (GFP, 1:500, Invitrogen #A6455) was added and the embryos were incubated overnight at 4°C with rocking ...
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bioRxiv - Biochemistry 2022Quote: ... The drug-selected recombinant CHO-S cells were adapted to serum-free suspension culture using CHO-S medium (CD Forti CHO; ThermoFisher). For large-scale cultures ...
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bioRxiv - Bioengineering 2021Quote: ... Expi™Sf9 cells were maintained as suspension cultures in flasks with serum-free ExpiSf™ CD Medium (Gibco, USA) at 27°C with stirring at a speed of 125 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... ATP-bound M854K MDA5 filaments were imaged at the MRC-LMB on a Titan Krios microscope with a K3 detector in CDS counting mode (ThermoFisher). Movies were recorded with a 1.4 s exposure time ...
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bioRxiv - Plant Biology 2021Quote: ... Each entry clone was then used to transfer the CDS into the destination vector pLEELA (Jakoby et al., 2004) or pDEST17 (Invitrogen) via the Gateway LR Clonase II Enzyme Mix (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... ISR1 CDS with a 5’ EcoRI restriction site and 3’ BamHI restriction site was amplified using Phusion (Thermo Fisher Scientific) and ISR1 start + EcoRI FOR and ISR1 no stop + BamHI REV primers (Table S1) ...
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bioRxiv - Biochemistry 2020Quote: ... full-length wild-type or mutant-coding sequences (CDS) of AtFN3K were cloned into pENTR/D-TOPO Gateway entry vector (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... NS0 cells were maintained in the same supplemented medium and were grown in CD Hybridoma chemically defined medium (Thermo Fisher) in a 37°C incubator with 125 rpm shaking and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). For the EMSA experiments ...
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bioRxiv - Plant Biology 2023Quote: ... Rice H4.V and H4 CDS were amplified (primers in Appendix Table S4) from cDNA prepared from seedling RNA with Superscript III reverse transcriptase (Invitrogen). H4.VS CDS was prepared by Gibson ligation of the PCR fragments from H4.V and H4 ...
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bioRxiv - Microbiology 2023Quote: ... rpsJ or rpsS CDS (IDT) were used as DNA templates for in vitro transcription with MEGAshortscript T7 High Yield Transcription Kit (Invitrogen). The transcripts were dephosphorylated with calf intestinal alkaline phosphatase (CIP ...
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bioRxiv - Cell Biology 2023Quote: ... APC8-mScarlet+/+ or RPE-1 FRT/TO APC8-mScarlet+/+ cell lines were cotransfected with the relevant gene CDS cloned into pcDNA5-FRT/TO (p1795) and pOG44 (Invitrogen) using a 1:5 ratio (using 1 µg total DNA per million cells) ...
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bioRxiv - Plant Biology 2023Quote: ... or DVL11 promoter fused to the DVL11 CDS were amplified and cloned in to the pCR8 vector using the pCR8/GW/TOPO TA Cloning Kit (Invitrogen). Then ...
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bioRxiv - Plant Biology 2022Quote: The ultraviolet–visible (UV–Vis) absorption spectrum of the CDs was recorded using the Multiskan GO microplate spectrophotometer (Thermo Scientific). The fluorescence measurements were performed using the FLS920 spectrofluorophotometer (Edinburgh Instruments ...
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bioRxiv - Plant Biology 2022Quote: ... The MpTIR1 CDS was then transferred into pMpGWB110 (Ishizaki et al., 2015) by using LR Clonase II (Thermo Fisher Scientific) to generate pMpGWB110_MpTIR1 ...
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bioRxiv - Plant Biology 2022Quote: The RBP45d coding DNA sequence (CDS) and PRP39a genomic DNA were cloned into the pENTR SD/D-TOPO entry vector (Invitrogen), and then recombined into the pGWB2 destination vector ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 ng of CTNND1.ex2 (gRNA_e2) or PKM.ex10 (CD) targeting gRNA and 200 ng of SRSF1 or SRSF3 expression vector using Lipofectamine 2000 (Life Technologies). Non-targeting (NT ...
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bioRxiv - Plant Biology 2024Quote: ... we amplified the full-length CDS of NPR1 and TGA3 and recombined into the intermediate vector pDonor207 using the BP recombination kit (Invitrogen), then cloned into the destination vector pEarleyGate101 with YFP-tagged sequences under the control of Pro35S using the LR recombination kit (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Movies were collected in Counted Super Resolution with 2x binning and CDS at a pixel size of 0.83 Å using EPU (ThermoFisher). An energy filter slit width of 20eV was used ...
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bioRxiv - Plant Biology 2024Quote: ... The coding sequence (CDS) of the Sr62TK was synthesised in pDONR207 and cloned into the pTA22-GW vector via Gateway LR reaction (Invitrogen). The CDS of Sr62-NLRa (Ae ...
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bioRxiv - Bioengineering 2024Quote: ... viable cell density was adjusted to 8×105 cells/mL in transfection medium: CD CHO medium (Gibco Cat. #10743-029) supplemented with 8 mM L-glutamine (Lonza Cat ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Bioengineering 2024Quote: ... and HITI insert were first mixed with a ratio of 2:2:1 with the reagent P3000 (ratio of DNA: P3000 of 1:2) in Opti-MEM™ Reduced Serum Medium (Gibco™), and then added to Opti-MEM™ Reduced Serum Medium (Gibco™ ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Genomics 2023Quote: ... coated dishes in N2 medium (high-glucose DMEM, 1% N-2, 2% B-27 and 1% penicillin/streptomycin, from Thermo Fisher Scientific) at a density of 2×105 cells/cm2 ...
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bioRxiv - Bioengineering 2024Quote: ... for 2 h and a 1 µg mL−1 solution of 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific 62248) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... HsTMEM120A was subjected to protease digestion with 1mg/ml proteinase K at 37°C for 1h (Thermo Scientific; EO0491). 0.2% SDS was added to the digestion solution to stimulate the activity of proteinase K ...
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1h at RT with AlexaFluor488-conjugated donkey anti-mouse IgG secondary antibody (0.2 µg/ml; ThermoFisher) diluted PBS-HS ...
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bioRxiv - Biophysics 2022Quote: ... Samples were incubated for 1h with a filtered and sonicated solution of red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) of 100nm (220nm filter ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Microbiology 2020Quote: ... for 1h at room temperature with gentle mixing followed by inactivation with 40U of SUPERase-In RNase inhibitor (Ambion). Cell extracts were passed through Sephacryl S400 spin columns (GE ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed twice in PBS-T for 5min and incubated for 1h with anti-rabbit AlexaFluor555 (Invitrogen, #A32794) or anti-rabbit AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Electrophoresis was carried out in MOPS buffer by applying a constant current of 50mA for 1h (Electrophoresis kit, Invitrogen). Tubulin concentrations were assessed either by using Coomassie staining or acetylated tubulin content ...
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bioRxiv - Neuroscience 2022Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphate buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Physiology 2022Quote: ... Hearts were again washed trice in PBT (0.2% Triton X-100 in PBS) for 1h each and incubated in blocking solution (10% FBS (4133, Invitrogen), 1% BSA (A7030-10G ...
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bioRxiv - Neuroscience 2024Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphat buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Evolutionary Biology 2023Quote: ... corresponding secondary antibodies were added for 1h at RT: (Alexa-594-conjugated goat anti-rabbit IgG (H+L) (Invitrogen), Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...