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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Protein concentrations of all extracts were measured using Pierce BCA Protein Assay Kit (Thermo Fisher, #23225). Samples for immunoprecipitation were prepared at a final concentration of 15 μg/μL protein in IP buffer up to 500 μL ...
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bioRxiv - Genomics 2019Quote: ... Magnetic protein A (120µl per IP) or protein G (180µl per IP) Dynabeads (both from Invitrogen) were washed with Wash buffer A (50mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was estimated by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL, USA). Denatured proteins were reduced with 5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA binding proteins were isolated using Pierce™ Magnetic RNA-Protein Pull-Down Kit (ThermoFisher, #20164). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Total tissue protein levels were measured with the Pierce® BCA Protein Assay kit (Thermo Scientific). Tissue immunoglobulin levels were normalized to total protein levels.
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bioRxiv - Immunology 2019Quote: ... Protein concentrations of the cell lysates were quantified using a BCA protein assay (Thermo Fisher Scientific) to ensure equal amounts of total protein were loaded in each well of NuPAGE 4–12% Bis-Tris Gel (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Abundant proteins were removed using the High Select Top14 Abundant Proteins Depletion Resin (Thermo Scientific A36372BR) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Protein concentration was determined by a Bradford protein assay using Coomassie plus assay reagent (Fisher Scientific). Optical densities were read on Fluostar (BMG LABTECH ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein containing fractions were checked for contaminants on NuPAGE 4-12 % Bis – Tris Protein Gels (Invitrogen), pulled ...
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bioRxiv - Synthetic Biology 2020Quote: ... Surface proteins were purified with the Pierce™ Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Purified surface proteins or total cell lysates were run on a 4–12 % BIS-TRIS gel (for P2X2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-protein complexes were then incubated with 40 µL pre-washed protein G Dynabeads (Thermo Fisher) for 2 hours ...
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bioRxiv - Bioengineering 2019Quote: ... Protein purity was assessed by SDS-PAGE using SYPRO™ Ruby Protein Gel Stain (ThermoFisher, S12000).
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bioRxiv - Cell Biology 2019Quote: ... 10 mg of total protein lysate were incubated for 3 h with protein A Dynabeads (Invitrogen) and 10 μg of MST2 antibody (ab52641 ...
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bioRxiv - Physiology 2019Quote: ... Protein content was measured using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) and samples containing 50 μg of protein were resolved by electrophoresis (7.5% SDS-PAGE) ...
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bioRxiv - Immunology 2019Quote: ... The concentrations of the protein lysates were measured using the BCA Protein Assay kit (Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... Total protein concentration was measured using the Pierce BCA Protein Assay kit (Thermo Scientific cat # 23225). The protein samples were then size-separated by electrophoresis on a gradient Bolt 4-12% Bis-Tris plus gel (Invitrogen NW04120BOX) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein concentration was measured using Pierce™ BCA™ protein assay kit (Thermo Fisher Scientific), 30 µg of total protein extract was used for western blotting ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher scientific Inc., Rockford, IL). CHT-mediated choline uptake was determined as total choline uptake minus the uptake in the presence of hemicholinium-3 and maximum transporter velocity (Vmax ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration of lysates was measured using the BCA Protein Assay kit (Thermo Fisher Scientific). For electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was quantified using the Pierce 660 nm Protein Assay Reagent (Thermo Fisher, Waltham, MA) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2021Quote: ... with MOPS buffer for proteins above 50kDa or MES buffer for proteins below 50kDa (Novex; Invitrogen). Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... ATP was normalized to protein content measured with the Pierce BCA protein assay kit (ThermoFisher Scientific). Triplicate technical replicates were performed for each sample ...
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bioRxiv - Neuroscience 2020Quote: ... protein contents for each sample were determined by BCA protein assay (Pierce) (Lot# RG235624; Thermo Scientific). Equal amounts of cerebellar lysates were mixed with denaturing 4X Laemmli loading buffer ...
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bioRxiv - Biochemistry 2021Quote: ... PageRule Unstained Broad Range Protein Ladder and PageRuler Plus Prestained Protein Ladder (both Thermo Fisher Scientific) were used as molecular-mass markers ...
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bioRxiv - Bioengineering 2020Quote: ... and protein concentration was quantified using a Micro BCA Protein Assay Kit (Thermo Fisher Scientific # 23235) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined by a BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) with bovine serum albumin as the standard.
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bioRxiv - Neuroscience 2020Quote: Hippocampal protein concentrations were determined according to the Pierce™ BCA Protein Assay (Thermo Fisher Scientific). Aliquots (20 μg ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of the supernatant was determined using BCA protein assay kit (Thermo Scientific, USA) and 100 µg were loaded to measure CCL2 levels by a specific ELISA kit (R&D Systems).
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentration in each sample was determined using the BCA protein assay (Thermo Fisher Scientific) and samples were denatured in 4X Laemmli sample buffer containing 10% β-mercaptoethanol for 10 minutes at 95°C ...
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bioRxiv - Cell Biology 2021Quote: Proteins were extracted from cellular lysates and quantified using the Qubit Protein Assay Kit platform (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged and 10 μg protein was separated on a NuPAGE 12% Bis-Tris protein gel (ThermoFisher) for histones and on a NuPAGE 4-12% Bis-Tris protein gel for non-histone proteins ...
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bioRxiv - Neuroscience 2019Quote: ... protein contents for each sample were determined by BCA protein assay (Pierce) (Lot# RG235624; Thermo Scientific). Equal amounts of cerebellar lysates were mixed with denaturing 4 Laemmli loading buffer ...
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bioRxiv - Physiology 2021Quote: ... The protein concentration in supernatants was measured using a Pierce BCA Protein Assay Kit (Thermo Scientific). Proteins were denatured in Laemmli buffer (62.5 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatants were collected and protein concentration was determined using a BCA protein Assay kit (Thermo Scientific). Cell lysates (eq ...
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bioRxiv - Physiology 2021Quote: ... Total protein was measured using the BCA method by Pierce™ BCA Protein Assay Kit (ThermoFisher). Samples (20-50 μg of total protein content ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was determined using the Pierce® Bicinchoninic Acid Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Proteins were resolved by SDS-polyacrylamide gel electrophoresis (NuPAGE 4-12 % Bis-Tris protein gels, Invitrogen) and detected by immunoblotting.
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bioRxiv - Microbiology 2020Quote: ... The total protein concentration was quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and protein content was determined using a Pierce BCA protein assay kit (Thermo Fisher Scientific #23225). Lysates were stored at −80°C until the day of the assay ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentration from each fraction was determined with Pierce Coomassie Plus (Bradford) Protein-Assay (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Total protein concentration was determined in the lysate using the Pierce BCA protein assay kit (Thermofisher) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2020Quote: Protein concentrations were determined using the BCA protein assay (Cat# PI23225, Thermo Fisher Scientific, Waltham, MA). Indicated samples were loaded on 4-20% Novex® gels (Cat# EC60285BOX ...
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bioRxiv - Microbiology 2021Quote: ... Total protein from lysates was quantitated using the Pierce Micro BCA protein assay kit (Thermo Scientific). Prior to gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... protein concentration was determined using the detergent tolerant Pierce™ BCA Protein Assay Kit (23227, ThermoFisher) according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2021Quote: ... Protein content of the supernatant was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts of protein were boiled in Laemmli buffer at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration of each supernatant was measured by the BCA protein assay (Thermo Fisher Scientific), and supernatants were diluted to equal protein concentrations ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay kit (Thermo scientific, #23225). Equal total protein concentrations were resolved on SDS-polyacrylamide gels (BioRad ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... the protein-antibody mixtures were incubated with magnetic protein-G beads (20 µl, Thermo Fisher Scientific) for another hour ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein amount was determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, 23225). The SDS gels (8% - 15% ...
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bioRxiv - Cancer Biology 2020Quote: Protein concentration from lysates and EV samples was determined using EZQ protein quantification Kit (Thermo Scientific). For mass spectrometry analysis ...