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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was done using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, 23225). For sample preparation ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was determined using Pierce BCA Protein Assay Kit (Cat No. 23325, ThermoFisher) according to the manufacturer’s manual.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was assessed using the Pierce Bicinchoninic acid (BCA) protein assay (Thermo Fisher #23225). Unless otherwise stated ...
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bioRxiv - Molecular Biology 2023Quote: ... protein concentrations of lysates after centrifugation were measured by BCA protein assay (Thermo Fisher Scientific). Lysates were diluted to equal protein concentrations in lysis buffer and then boiled in SDS sample buffer (20 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... and quantified for protein concentrations using the Pierce BCA protein assay kit (Thermofisher, Cat# A55864) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Total protein concentration was determined using Pierce BCA protein assay kit (Catalog# 23225, Thermo Scientific), and equivalent protein fractions were diluted in cell lysis buffe ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein was quantified with a Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific) and diluted to 0.4 µg/µL final concentration including lx Master Mix (from EZ Standard Pack l (PS-ST0lEZ-8 ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein concentration was determined by spectrophotometry using Pierce™ BCA Protein Assay Kits (ThermoFisher Scientific). Equal amounts (40 μg ...
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bioRxiv - Cell Biology 2024Quote: ... the immunoprecipitates were conjugated with protein G Beads (Dynabeads Protein G, Thermo Fisher Scientific 10004D), eluted in Laemmli buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein concentration was measured with the Pierce BCA Protein Assay Kit (Thermo Scientific, 23225). Lysates were resolved on SDS-PAGE and transferred electrophoretically to 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured with the Pierce TM BCA Protein Assay Kit (ThermoFisher Scientific, USA). Proteins were separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Protein band sizes (kDa) was visualized using the SeeBlue Plus2 pre-stained protein standard (ThermoFisher). For western blot ...
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bioRxiv - Bioengineering 2024Quote: Protein concentrations were obtained with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Catalog # 23225), and purified protein was frozen in aliquots on dry ice ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentrations were determined with the BCA protein assay kit (23235, Thermo Fisher Scientific). Thirty micrograms of total cell lysate were loaded on 10% SDS–polyacrylamide gel electrophoresis gels ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the bicinchoninic acid assay (BCA protein assay, Thermo Fisher Scientific). Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentrations were measured using the Micro BCA Protein Assay Kit (#23235, ThermoFisher Scientific). The protein concentration determined for each individual retina varied from 4µg/µL to 5µg/µL ...
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bioRxiv - Cell Biology 2024Quote: ... Protein quantification was performed by using the Pierce BCA protein assay kit (Thermo Scientific, 23227) according to the user manual ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with the Pierce™ BCA Protein Assay Kit (Thermo Scientific 23225). 15 µg of protein sample was run on precast 4-15% gradient Tris-Glycine gels (BioRad 4561084 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the total protein concentration quantified with the Micro BCA Protein Assay Kit (Thermo Scientific). Lysates were prepared in SDS sample buffer ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using a Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No: 23227) and standardized ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration in each sample was determined by BCA protein assay (Thermo Fisher Scientific) and samples were denaturated in 4x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined and normalized using a BCA protein assay (Thermo Fisher Scientific, 23225). Equal amounts of protein by weight were combined with Laemmli buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), and equal quantities of protein were mixed with Laemmli buffer or 5x Pierce Lane Marker Reducing Sample Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Total protein was quantified using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, USA). Calprotectin was normalized to initial stool weight (ng calprotectin/mg stool).
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was quantified using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were quantified by the BCA Protein Assay Kit (Thermo Fisher Scientific, Cat# 23223). Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific Inc, 78501), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Inc, 23225), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23225). Lysates were separated by SDS-PAGE and blotted onto a nitrocellulose membrane (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: ... Protein concentration in each extract was measured using PierceTM BCA Protein Assay Kit (Thermo Scientific). Laemmli buffer (Biorad ...
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bioRxiv - Physiology 2024Quote: ... Samples with equal amounts of proteins were precleared with Dynabeads Protein G (Thermo Fisher Scientific) for 2 hours at 4 °C and then incubated with 3 µg of primary antibody on a rotary platform overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay Kit (Cat#: 23227, Thermo Scientific). Samples were electrophoresed on polyacrylamide gels (Cat# NW04120BOX ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified by BCA Assay (Pierce™ BCA Protein Assay Kit, 23225; via ThermoFisher). Serum albumin was used as the standard.
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bioRxiv - Cell Biology 2024Quote: ... 4mM EDTA) and protein content was accessed using BCA Protein Assay (Thermo Fisher Scientific #23225). Then 5μg of protein was incubated with 1X Reaction Buffer ...
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bioRxiv - Genetics 2024Quote: ... Total protein concentrations of lysates were quantified by BCA Protein Assay (Thermo Fisher Scientific, #23225) and each sample was analyzed in technical duplicates for apo(a ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were measured with the Pierce 660 nm Protein Assay (Thermo Fisher Scientific, 22660) and 500 μg of protein per sample was used for subsequent mass spectrometry experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo-Fisher Scientific, Cat #23225). After concentration adjustment ...
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bioRxiv - Biochemistry 2024Quote: ... supernatants removed and protein concentration determined via the Pierce BCA Protein Assay Kit (Thermo Fisher). The absorbance of each sample was read at a wavelength of 562 nm (Infinite 2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the Pierce BCA protein assay kit (Thermo Scientific™, 23225). Following SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined using BCA assay (23225, Pierce™ BCA Protein Assay Kit, Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... We combined 4 mg of Cas9 Nuclease protein (TrueCut Cas9 Protein V2, Thermo Fisher Scientific), 5 mL of Neon Buffer R (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissolved protein was analyzed for protein concentration by micro-scale BCA assay (Micro BCA Protein Assay Kit #23225; Thermo Fisher Scientific Inc., Waltham, MA, USA). Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).