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Citations for Thermo Fisher :
7201 - 7250 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 3 million cells were washed twice with Opti-MEM (Gibco) and resuspended in 90 µl Opti-MEM with 10 µg of DNA in an electroporation cuvette with a 2 mm gap ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected on 3 μg/ml blasticidin (Gibco, A1113903) for 3 days ...
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bioRxiv - Immunology 2022Quote: ... and suspended in Fc Block for 5 min at RT prior to staining with a surface stain of following antibodies: CD3 (Invitrogen, 45-0031-82)/CD4 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of glucose in filtrate was quantified using Glucose CII-Test Wako (FUJIFILM Wako Pure Chemical Corporation) by measuring the absorbance at 492 nm with a Thermo Scientific Multiscan® FC (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 340 nm was read every 12 s at 37°C using a Multiskan FC plate-reader (Thermo Scientific, Paisley, UK). Maximum absorbance ...
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bioRxiv - Microbiology 2020Quote: ... Then the resultant supernatants were transferred to a 96-well plate for the absorbance measurement at 492 nm with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA). RBCs treated with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Planktonic cell growth was determined by quantifying optical densities at 595 nm using a microplate spectrophotometer (Thermo scientific multiscan FC: Cat no. 51119000). Plates were thoroughly washed three times with 200 µl of sterile PBS per well using a microplate washer (Micro wash 1100 from Global diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended at a concentration of 1 × 106 cells/mL in RPMI without FCS and labeled with CellTracker™ Blue CMAC dye (10 μM, Thermo Fisher, C2110) for 20 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 cells were maintained in DMEM media supplemented with FCS (10 % final concentration) and Penicillin Streptavidin-Glutamine (PSG; Gibco, 1 % final concentration). For starvation ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... using DMEM (4.5 g/l glucose) with 2 mM Glutamax-I medium supplemented with 15% foetal calf serum (FCS) (ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 50nM Aβ-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The absorbance reading was conducted at 595 nm using a microplate photometer (IVD model, MultiskanTM FC, Thermo Fisher Scientific Inc., MA, United States).
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bioRxiv - Microbiology 2023Quote: ... Hungary) substrate and the optical density of the solution was measured at 450 nm using a Multiscan FC reader (Thermo Fisher Scientific Inc.). Blood samples were centrifuged after collection and the sera were kept at −70°C ...
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bioRxiv - Cell Biology 2023Quote: ... using either a WPA CO8000 Cell Density Meter (Biochrom, Cambridge, UK) or a Multiskan FC microplate reader with a 594 nm filter (Thermofisher, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The single cell suspension was incubated with 1:1000 Fc Block (AbLab) in 1X FACS Buffer and stained for surface markers before being fixed/permeabilized (Thermofisher 00-5523-00) for FoxP3 staining (invitrogen 11-5773-82 Table 2).
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...
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bioRxiv - Biophysics 2023Quote: ... setup alignment and quantifying confocal volume parameters were performed with fluorescence correlation spectroscopy (FCS) using ATTO425-COOH, ATTO488-COOH, Alexa Fluor 546 or ATTO655-COOH (ATTO-TEC GmbH, Siegen, Germany) (Alexa Fluor™, Thermofisher Scientific). Data was analyzed using PAM-FCSfit (Supplemental Figure S5A) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Proteins were then quantified with Image Studio Version 5.2.4 (LI-COR Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... Phage pools representing the libraries were cycled through rounds of binding selections with EPHA2-FN2-Fc fusion protein immobilised on Maxisorp Immuno plates (ThermoFisher, #12-565-135), as described previously [21] ...
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bioRxiv - Systems Biology 2023Quote: Murine 3T3-L1 preadipocytes differentiated into mature adipocytes by first incubating confluent cells in induction-medium (DMEM GlutaMAX™, Gibco®, 10% FCS) containing 1.6μM insulin ...
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bioRxiv - Microbiology 2024Quote: ... The isolated cells were resuspended in DMEM supplemented with 5% FCS and immediately plated on glass coverslips using Cytofunnels (Thermo Fisher Scientific, USA) and centrifugation at 1500g for 10 minutes with a Cytospin 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... After lysis of red blood cells with Gibco ACK lysing buffer and blocking of Fc receptors (Invitrogen CD16/CD32 clone 93, RRID AB_467133), cells were stained with live/dead aqua (Thermo ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 50nM Aß-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Microbiology 2020Quote: Toxicity of the surfaces was tested on human immortalized HaCaT keratinocytes (ThermoFisher). Cells were maintained in DMEM high glucose medium supplemented with Na-pyruvate (1mM) ...
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bioRxiv - Cell Biology 2020Quote: ... 293T and human secondary fibroblasts cells were cultured in DMEM (Gibco, 11995) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... in tissue culture plates coated with 2.5μg/cm2 human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 cells (human colorectal epithelial) were maintained using DMEM (Gibco, 41966-029) supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPS WT-11 cells were cultured on Vitronectin (Thermo Fisher Scientific) coated 6-well plates or glass coverslips (for smRNA FISH purposes ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated in goat anti-human HRP secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution in blocking buffer for 45-60 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Biochemistry 2022Quote: ... 100 units/ml human LIF supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Labelled DNA was then hybridized to Clariom S human GeneChips (ThermoFisher Scientific) and incubated at 45℃ in the GeneChip Hybridization oven 640 (Affymetrix ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
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bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc. ...
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bioRxiv - Immunology 2020Quote: ... Cross-adsorbed goat anti-human IgG (H+L) secondary antibody (A18811, Invitrogen) was used at a dilution of 1:3,000.
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bioRxiv - Genetics 2020Quote: ... and quantified using the Quantifiler Human DNA Quantification kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng/ml of recombinant human epidermal growth factor (EGF, Life Technologies), human insulin (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 goat anti-human IgG (H+L) (Molecular Probes, A21445) (1:500 IF) ...