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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... The cells were selected for three passages in DMEM supplemented with 10% FCS and 500 μg/mL G418 sulfate (ThermoFisher Scientific, 10131027). The extent of overexpression was assessed with flow cytometry (Suppl ...
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bioRxiv - Bioengineering 2023Quote: ... TF-1 were purchased from DSMZ (Cat#ACC334) and maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies), 2mM GlutaMAX and 2ng/ml hGM-CSF (215-GM ...
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bioRxiv - Cancer Biology 2023Quote: ... Uveal melanoma cell lines were maintained in RPMI and cutaneous melanoma cells and HeLa cells were cultured in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). The MA104/NSP5-BioID2 cell line was generated using a lentiviral system ...
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bioRxiv - Genomics 2023Quote: Lymphoblastoid cell lines (LCLs) from healthy donors and ICF2 patients were cultured in RPMI 1640 supplemented with 15% FCS and antibiotics (Thermo Fisher Scientific) as described in (15).
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated with 5 μg/mL anti-mouse CD16/CD32 Fc block (clone 2.4G2, Thermo Fisher Scientific, Cat. #553142) for 15 minutes at 4°C per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... cells were kept in MEM Alpha Medium supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (Invitrogen GmbH, Darmstadt, Germany) in a humidified atmosphere at 5% CO2 and 37°C ...
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bioRxiv - Physiology 2023Quote: ... The formazan crystal pellets were dissolved in DMSO and the plate was read 10 min later using Multiskan FC Microplate Photometer (Thermo Fisher, USA) at 550 nm.
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bioRxiv - Immunology 2023Quote: ... and resuspended in complete medium (RPMI 1640 supplemented with 10% fetal calf serum, FCS, 100 U/mL penicillin and 100 μg/mL streptomycin; Sigma-Aldrich and Invitrogen respectively). After the isolation ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from mice tibias and femurs as described previously29 and cultured for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FCS (Thermo Scientific, Rockford, IL, USA), penicillin-streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... After incubation at 37 °C for 30 minutes the mixture was transferred to a 96-well plate and turbidity was measured by absorption at 620 nm using a Multiskan FC microplate reader (Thermo Fisher, USA). All samples were examined in triplicates (n = 3).
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bioRxiv - Molecular Biology 2024Quote: ... HEK293FT and HeLa cells were cultured under standard conditions (DMEM; 10 % FCS, 1 % penicillin/streptomycin, all from Invitrogen; 37 °C; 5 % CO2). The HeLa Denr knockout (KO ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% fetal calf serum (FCS)(AMIMED, BioConcept, Switzerland) and penicillin (100 U/ml)-streptomycin (100 µg/ml)(Gibco, Life Technologies). MA/cytBirA (24 ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Immunology 2019Quote: ... and IL-2 concentration was measured using a mouse IL-2 ELISA kit (Invitrogen). For CFSE dilution assays ...
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bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Candidate drugs were diluted 2-fold seriously by medium supplemented with 2% FBS (GIBCO), penicillin (100 IU/mL) ...
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bioRxiv - Microbiology 2021Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850); (3 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg/ml goat anti-rabbit F(ab’)2 fragment (Life Technologies A-21246) and goat anti-mouse F(ab’)2 fragment secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed three times with 2× saline sodium citrate (2×SSC, ThermoFisher AM9765), and equilibrated with encoding-probe wash buffer (30% formamide (ThermoFisher AM9342 ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.4) supplemented with 2 μM fura-2-AM (F1201, Molecular Probes, Eugene, OR) and 0.01 % pluronic acid ...
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bioRxiv - Immunology 2020Quote: ... Fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D21490, 2 μg/mL), wheat germ agglutinin (WGA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 cells were seeded into LabTekII 2-well chambered coverglass (#155380, ThermoFisher Scientific) pre-coated with 0.01% Poly-L-lysine (#P4707 ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D21490, 2 μg/mL), wheat germ agglutinin (WGA ...
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bioRxiv - Microbiology 2021Quote: ... 2% fetal bovine serum (S182H-500, Eurobio) and N-2 Supplement (17502048, Thermo Fisher) at 37°C and 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl 10% SDS plus 2 µl proteinase K (Thermo Fisher Scientific, Cat# EO0492) were added and incubated for 60 min 37°C ...
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bioRxiv - Cell Biology 2024Quote: Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes (Leiden, The Netherlands). High-glucose Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/ml of fibroblast growth factor-2 (FGF-2) (all from Gibco). Cells were seeded at a density of 3200 cells/cm2 until they reached 90% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... and 5ng/mL bovine fibroblast growth factor-2 (bFGF-2, #13256-029, Gibco™)) (proliferative conditions) ...
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bioRxiv - Cancer Biology 2023Quote: ... animals were subjected to intracardiac perfusion with 2% PFA-2% glutaraldehyde (A17876, ThermoFisher Scientific) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Genomics 2023Quote: ... and 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Gibco 15630080), and incubated at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 1:20-1:100 (volume) of 1 mg/ml fluorescent tag (Alexa Fluor® succinimidyl esters of the desired excitation/emission spectrum, Invitrogen™, Molecular Probes®) to the gel premix immediately before polymerization.
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bioRxiv - Zoology 2021Quote: ... 150 μg of each lysate was digested with trypsin and labelled with Tandem Mass Tag (TMT) 11-Plex reagent kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). In order to normalize across multiple TMT kits ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... the peptide mixture was labeled with TMT 10-plex and 11-plex isobaric tandem mass tags (catalog no. 90110 and A34808, Thermo Fisher Scientific, Rockford, USA) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Mex67 or Sac3 under the control of the GAL1 promoter and carrying a HA C-terminal tag (Gelperin et al., 2005) were obtained from the Yeast ORF collection (Thermo Scientific Open Biosystems, YSC3868). pGP565 2µ multicopy plasmids (Jones et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell expressing protein fragments with an MBP tag were lysed first using 1 mL Bacterial Protein Extraction Reagent (B-PER; Thermo Scientific Catalog number: 78243) and 1-2x of protease inhibitor (Thermo Scientific A32963) ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-GFP (Sicgen Antibodies, Cantanhede, Portugal; AB0020) and TAMRA fluorescence tag was intensified with an Anti-TAMRA antibody (Thermo Fisher Scientific; MA1-041) and incubated ON at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Immunology 2023Quote: ... blocked with PBS-T containing 3% BSA for 1 hour at room temperature and incubated at 4°C overnight with 1 μg/ml biotinylated-camelid α-C-Tag-specific antibody (Thermo Fisher Scientific, #7103252500) in PBS-T ...