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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 50μl/well 10% HCl and the absorbance was measured at 492nm (A492) using a Multiskan FC reader (Thermo Fisher Scientific). In every CovIgG-Assay determination two in-house controls ...
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bioRxiv - Cancer Biology 2021Quote: Cells were washed with PBS and resuspended in 100% FCS and cytospun onto coated slides using a Shandon Cytospin 4 (Thermo Scientific) at 30g for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Daudi and HL60 cells were cultured in RPMI media supplemented with 10% fetal calf serum (FCS) and 1% pen/strep (all from Thermo Fisher). Phoenix-Ampho cells were cultured in DMEM medium (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... The reaction was stopped by adding 35 μl of 1 M sulfuric acid (H2SO4) and the optical density was measured at a wavelength of 450 (OD450) (MULTISKAN FC, Thermo scientific).
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein bands were visualized using a LI-COR Odyssey FC Imaging system and Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher). Western blots were quantified using Image Studio Software ...
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bioRxiv - Microbiology 2019Quote: ... Large scale transient transfections for murine CRD4-7-Fc and CR-Fc were carried out using 100 µg of total plasmid DNA and 100 ml of suspension cultured Expi293F cells (Life Technologies). Supernatants were harvested at day 6 ...
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bioRxiv - Immunology 2019Quote: ... The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Microbiology 2019Quote: ... or 0.2 (for RNA sequencing and western blots) and cultured in RPMI-HEPES supplemented with 5% v/v fetal calf serum (FCS) (Invitrogen, Australia) at pH 8.1-8.2 and allowed to differentiate at 37 °C with ambient CO2 49 ...
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bioRxiv - Cell Biology 2021Quote: ... and were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% FCS and 100 μg/mL of hygromycin B (Invitrogen, 10687010).
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bioRxiv - Neuroscience 2020Quote: ... washed and non-specific antibody binding was minimised by incubation for 30 min at room temperature in PBS containing 10% FCS and 0.05% Triton X-100 (all Thermofisher Scientific, UK). Cells were then incubated with rabbit anti-mouse p67Phox monoclonal antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown in a single well of a six-well plate in 10% FCS-MEM and transfected with 3 μg of BAC plasmid with Lipofectamine 3,000 (Thermo Fisher, USA). After transfection ...
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bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
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bioRxiv - Microbiology 2021Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (40 ...
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bioRxiv - Microbiology 2021Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (40 ...
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bioRxiv - Microbiology 2021Quote: ... supernatants were loaded into a 5 mL SepFast Ø11mm (Quimigen) packed with CaptureSelect™ IgG-Fc (ms) affinity resin (Thermo Scientific) connected to an Äkta Start Chromatograph (Cytiva) ...
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bioRxiv - Microbiology 2020Quote: ... 125 µl was transferred into a clean 96 well Costar plate to measure the absorbance at 595 nm using a Multiskan FC (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Sections were soaked in PBS for 10 minutes before incubation in blocking solution (20% fetal calf serum (FCS, Thermo Fisher Scientific), and 20% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ~3 mL of serum from CV-A21-immunized or naïve mice was diluted in 97 mL of PBS and ran over a column pre-packed with CaptureSelect IgG-Fc (Multispecies) Affinity Matrix (ThermoFisher Scientific). IgGs were eluted with 0.1 M glycine buffer (pH 3.0 ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured in F-12K Nut Mix medium supplemented with 5% (v/v) artificial FCS and with 0.5% (v/v) Insulin- Transferrin-Selenium (Thermo Fisher Scientific). BEAS-2B (Cat# CRL-9609TM ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... HCT116 colorectal adenocarcinoma cells were maintained in McCoy’s medium supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO, Cat#16141079), 10 U/ml penicillin/streptomycin ...
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bioRxiv - Biophysics 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
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bioRxiv - Microbiology 2020Quote: ... was used to detect circulating antibodies for LSDV in serum samples following the manufacturer’s protocol and analysed with the Multiskan FC Microplate photometer (Thermo Scientific™). Infectious virus titrations of PBMC suspension ...
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bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
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bioRxiv - Microbiology 2022Quote: ... containing 10% fetal calf serum (FCS) (AMIMED; BioConcept, Switzerland) and penicillin (100 U/ml)–streptomycin (100 μg/ml) (Gibco, Life Technologies). MA/cytBirA and MA/NSP2-mCherry (59 ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Immunology 2020Quote: ... Activated CD8+ T cells were maintained in DMEM medium (10% FCS) with 25 mM (HG) or 5.6 mM (NG) glucose (Thermo Fisher Scientific). Raji cells were maintained in RPMI 1640 medium (10% FCS ...
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bioRxiv - Molecular Biology 2021Quote: ... Interactions were visualized by the incubation of tetramethylrhodamine (TRITC) labelled secondary goat anti-mouse IgG antibodies (Fc specific; 1:1000 dilution in binding buffer; Life Technologies) and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... Growth of each microbe was assessed by measurement of optical density (OD) readings at 600nm using a Multiskan FC Microplate Photometer (Thermo Scientific). Bacterial density in each well in the parent plate was then normalized individually to an OD600 of 1.0 using sterile filtered M9 buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... iPSCs were cultured in KSR basal medium (GMEM basal medium with 10% KSR, 1% FCS) or N2B27 basal medium (DMEM/F12 and Neurobasal (ThermoFisher Scientific) in a 1:1 ratio ...
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bioRxiv - Cell Biology 2021Quote: ... Inserts were gently washed and placed in serum free DMEM and the upper chamber filled with DMEM supplemented with 10 % FCS (Life Technologies) and either PBS or 100ng/ml FGF10 (Peprotech) ...
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
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bioRxiv - Molecular Biology 2019Quote: ... female) cells and C2C12 (ATCC® CRL-1772™) cells were maintained in DMEM supplemented with 10% FCS (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The amount of neutral red taken up was determined by measuring the absorbance at 540 nm using a Multiskan FC Microplate Photometer (Fisher Scientific). The CC50 values were calculated from best-fit dose response curves with variable slope in Prism 8.
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in RPMI 1640 medium containing 10% FCS was labelled with 10μM CFSE dye (Thermo Fisher Scientific, catalogue# C34570) for 4 minutes at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were cultured in Dulbecco’s Modified Eagle Medium (DMEM, High Glucose, GlutaMAX™, Pyruvate) supplemented with antibiotics and 10% FCS (Gibco, Life technologies) and maintained in T75 Flasks at 1×106 cells per flask ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were resuspended at a concentration of 2.5-5 × 106 cell/mL in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (F14201, Thermo Fisher Scientific) and 3 μM Fura Red (F3021 ...
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bioRxiv - Immunology 2020Quote: ... Absorbance was measured at 450 nm with a reference wavelength of 620 nm using the Multiskan FC Microplate Photometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... cells were adjusted to 10.106 -20.106 /mL in FACS buffer (PBS, 1% FCS, 5% Hepes, 0.1% Azide) containing SYTOXTM Orange Dead Cell Stain (recommended dilution, Thermo Fisher Scientific) for nuclear staining ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were mechanically homogenised into single cell suspensions in RF10 media (RPMI 1640, 10% FCS, 1× penicillin-streptomycin-glutamine; Life Technologies). Isolated cells were stained with Aqua viability dye (Thermofisher ...
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bioRxiv - Genomics 2021Quote: ... Live cells were identified by washing after Fc block and incubation with Fixable Viability Dye Violet (Invitrogen, cat. L34955, 1:1000) prior to surface staining or incubation with calcein blue (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Neuroscience 2022Quote: ... SH-SY5Y cells were replated into primary astrocytes in 6-well plates in density 1.103cells/cm2and co-cultivated for further 5 days in DMEM medium (PAA Laboratories GmbH, Germany) containing 1% FCS (Invitrogen, Carlsbad, CA), 2 mM L-glutamine (PAA Laboratories GmbH ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellets were resuspended in 100 μL of FC buffer containing LIVE/DEAD violet dye (Thermo Fisher, 1:1,000 dilution) and secondary antibodies (goat anti-mouse IgG Alexa 647 conjugated ...
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bioRxiv - Physiology 2024Quote: ... The optical density of the reaction product was read at 450 nm using the Multiskan FC microplate reader (Thermo Scientific, USA) after the initial incubation period (3 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
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bioRxiv - Neuroscience 2024Quote: ... DRGs or TGs were isolated and digested with 1 mg/ml collagenase and 2.5 mg/ml dispase dissolved in ‘basal medium’ (Neurobasal A medium supplemented with 10% FCS) (all from Gibco/Thermo Fisher) at 37°C for ca ...