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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: All of the PCR reactions were performed using an ABI Prism 7900HT Sequence Detection system (Thermo Fisher Scientific, Waltham, Massachusetts). PCR was performed using the Power SYBR™ Green PCR Master Mix (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Cycling was performed in duplicate using a Step One Plus™ Real Time detection system (Applied Biosystems, Foster City, CA). Gene-specific amplification was normalized to GAPDH as an internal reference gene and gene expression was normalized relative to uninfected MDSC controls using the formula 2-ΔΔCt.
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems), using System SYBR-green fluorescence (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... and GFAP using the Applied Biosystems™ ABI PRISM™ 7000 Sequence Detection System (Applied Biosystems, ThermoFisher Scientific, Waltham, MA) and Rotor-Gene Q (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Feature detection and peak alignment from .Raw files were performed using Compound Discoverer 3.2 (Thermo Fisher Scientific, Waltham, MA, USA). Files were also converted to .mgf format using MSConvert software (ProteoWizard ...
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bioRxiv - Neuroscience 2020Quote: Glutamate concentration in microdialysis samples was quantified using isocratic high-performance liquid chromatography (HPLC) with electrochemical detection (Thermo Fisher Scientific). O-pthalaldehyde (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... was used for the PCR reaction was used according to the manufacturer’s protocol in a QuantStudio3 Detection System (Applied Biosystems). Each sample was run in duplicate and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... followed by internalization into target cells and detection by incubation with secondary anti-human-Alexa 555 fluorophore conjugates (Thermo Scientific). Cells were examined by immunofluorescence analysis performed on an Axio Observer Z1 widefield epifluorescence microscope (Zeiss Inc. ...
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bioRxiv - Cell Biology 2020Quote: To detect proliferation/cell cycle entry we utilized the EdU/click-it detection platform to visualize cells that contained newly synthesized DNA (Cat nb. C10337 or C10340, Invitrogen, Thermofisher). For experiments performed immediately post FACS ...
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bioRxiv - Cell Biology 2021Quote: ... MS-HRM-PCR analysis was done in 7500 FAST Real Time ABI Prism Sequence Detection System (Applied Biosystems, Boston, EUA) using temperature ramp and acquisition of fluorescence in accordance with the manufacturers’ recommendation ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression was assessed from 10 ng cDNA at the ABI 7900 HT system using SYBR green detection and the SDS (v2.4) software (Applied Biosystems). For normalization the two housekeeping genes HPRT1 and GNB2L1 were used ...
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bioRxiv - Bioengineering 2022Quote: ... conjugated to horseradish peroxidase (HRP) were used for detection of proteins using ECL Western Blot Substrate Reagent (Thermo Fisher Scientific). Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... Blots were visualized using the Pierce ECL 2 Western Blotting Substrate detection method (0.5 pg, lower limit sensitivity; Thermo Scientific). The images were obtained and analyzed with ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time RT-PCR measurements were performed using the Step One Plus Real-Time Detection System (Thermo Scientific, Waltham, USA). Reactions were performed in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... All QPCR reactions were performed in duplicate on the 7500 ABI Prism Sequence detection System (Applied Biosystems, Foster City, CA). The cycling conditions included a denaturation step of 95°C for 10 mins followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in an ABI StepOnePlus instrument with the SYBR green detection system (Invitrogen). PCR thermal profiles were 40 cycles with 10 s at 95 °C and 30 s at 60 °C each cycle ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Microbiology 2020Quote: ... The thermal cycle and fluorescence detection were performed with the QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using a Step One Plus detection system (Applied Biosystems, Life technologies, the Netherlands) using Taqman assays (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... The arrays were washed again with distilled water (G biosciences) and incubated with a detection buffer with Cy5-streptavidin (ThermoFisher) at 1:1000 for 20 minutes at RT protected from light ...
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: All quantitative PCR (qPCR) experiments were conducted using an ABI 7900HT sequence detection system (Perkin-Elmer Applied Biosystems, Warrington, UK). Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunofluorescence detection in confocal and SIM microscopy were Alexa Fluor Rabbit-anti-Mouse 568 (Invitrogen, A11004) and Donkey-anti-Mouse 647 (Invitrogen ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Real Time qRT-PCR was performed using SYBR-green based detection on a Quant Studio 3 real time PCR system using PowerUP Syber green MasterMix (ThermoFisher). Primers for GAPDH and beta-actin were used as endogenous controls to which RNA levels of target genes were normalized to ...
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bioRxiv - Evolutionary Biology 2022Quote: HPLC with mass spectrometry detection was done on a quadrupole LC-MS (Ultimate 3000 UPLC/ISQ EC, Thermo Fisher Scientific) in Single Ion Monitoring (SIM ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody detection was performed with Alexa Flour 488 goat anti-mouse and Alexa Fluor 594 goat anti-rabbit (Invitrogen). Cells were counterstained with DAPI (1:10,000 ...
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bioRxiv - Genetics 2019Quote: ... and mouse-anti-Prospero (DSHB) followed by secondary detection with anti-rabbit or anti-chicken Alexa 488 and anti-mouse Alexa 555 (Invitrogen).
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bioRxiv - Pathology 2019Quote: ... Sections for the detection of autoantibodies accumulation were blocked and permeabilized with 0.5% Triton X-100 (85111, Thermo Fisher Scientific) and blocked with 2.5% bovine serum albumin solution (BSA ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed on a Step-One Plus Real-Time PCR Detection System (Applied Biosystems, Waltham, MA, USA) using 2X Power SYBR Green Mastermix (ABI ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies were detected with peroxidase conjugated goat secondary antibody and enhanced chemiluminescence (ECL) western blotting detection reagent (Thermo Scientific).
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bioRxiv - Genomics 2020Quote: ... The amplification and detection of specific DNA was achieved using the AB 7500 FAST Real-Time PCR System (Applied Biosystems) and the following programme ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were visualized by chemiluminescent detection (Image Quant) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... Detection was accomplished with a secondary stain of Alexa Fluor 488 and Alexa Fluor 594 anti-mouse IgG antibodies (Invitrogen). Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run in duplicate for each sample and quantified using the ABI Prism 7900 sequence detection system (Applied Biosystems). Duplicates were checked for reproducibility ...
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bioRxiv - Biochemistry 2020Quote: ... The quantitative RT-PCR reactions were performed on an Applied Biosystems 7500 Sequence Detection system (Applied Biosystems, Foster, CA, USA) using ChamQ SYBR Color qPCR Master Mix (Nanjing Vazyme BioTech Co.,Ltd.) ...
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bioRxiv - Molecular Biology 2021Quote: ... Visual detection was accomplished by imaging the tubes through E-Gel™ Safe Imager™ Real-Time Transilluminator (Thermo Fisher).
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bioRxiv - Synthetic Biology 2021Quote: ... Real‐time PCR amplification was performed on 12.5 ng cDNA with the ABI Prism 7300 sequence detection system (Applied Biosystems), using the PowerUp™ SYBR® Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were washed a further 3 times for 10 min in TBS with 0.2% Tween 20 and prepared for chemiluminescent signal detection using SuperSignal West Pico chemiluminescent substrate (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... ND4 and APP was performed simultaneously within the same well using a 7500 fast sequence detection system (Thermo Fisher Scientific). Efficiency of the triplex reaction was measured prior sample analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred onto PVDF membrane (Cytiva) and detected using either enhanced chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate; ThermoFisher) or fluorescent secondary antibody (ThermoFisher) ...
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bioRxiv - Pathology 2021Quote: ... 35 mins in MES buffer for aSyn detection) and transferred to nitrocellulose membranes (0.2 μm) via Iblot (7 mins, 20 mV; Fisher Scientific). Initial blots were tested for optimisations of aSyn retention as labelled by MJFR1 either by pre-blocking heat treatment (microwaved ...
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bioRxiv - Genomics 2020Quote: ... before membrane washing and incubation with appropriate HRP-conjugated secondary antibodies (Amersham) and detection with SuperSignal ECL West Pico PLUS chemiluminescence (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... Quantification of these genes was performed using SYBR Green on a ViiA™ 7 sequence detection system (Applied Biosystems, USA) with two replications for each sample ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction was performed using the ABI Prism 7000 Sequence Detection System (Life Technologies, Carlsbad, CA). Run method ...
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bioRxiv - Cell Biology 2022Quote: ... The quantification of PCR products was analyzed with SYBR green using ABI PRISM 7900 Sequence Detection system software (Applied Biosystems). The primer sequences were MajSAT 5’-GGCGAGAAAACTGAAAATCACG-3’,5’-CTTGCCATATTCCACGTCCT-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection probes for phage DNA in Southern blots were generated by PCR using a non-proofreading polymerase (DreamTaq polymerase, ThermoFisher) using oligonucleotides specified in Table S4 ...