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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... First strand cDNA synthesis was carried out from 1 μL total RNA at 1 μg/μL with random hexamer primers and Superscript III RT kit (Invitrogen Inc., Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The psf model includes optical aberrations of the experimental setup retrieved from z-stack measurements of 100 nm fluorescent beads (TetraSpeck Microspheres, Thermo Fisher Scientific, USA). The defocus value was adjusted in the software so that the lowest localizations in z appeared close to 0 ...
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bioRxiv - Cell Biology 2020Quote: M cell mediated uptake was determined by confocal microscopy of apically exposed 2.0μm fluorescent polystyrene latex beads (Life Technologies; 4×108 final concentration) to human enteroid monolayers expressing or lacking M cells that were cultured in the presence or absence of 106 pathogenic bacteria overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Each slide was then placed in light-tight humidity control tray and Blue Fluorescent Nissl stain (Molecular Probes Neurotrace 435/455; Invitrogen Cat N21479) diluted in blocking solution (1:250 ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial mass levels and specific mitochondrial superoxide levels in the absence or presence of mitoTEMPO were quantified with the fluorescent probes MitoTracker™ Green FM (ThermoFisher Scientific, M7514) and MitoSOX™ Red (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescent beads (either dark red, excitation 660 nm / emission 680 nm; or orange, excitation 540 nm / emission 560 nm; Thermo Fisher Scientific, F10720) were diluted 1:5000 in mQH2O ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Systems Biology 2021Quote: The relative mitochondrial transmembrane potential (ΔΨm) was measured using the membrane-potential-dependent fluorescent dye TMRE (Tetramethylrhodamine, Ethyl Ester, Perchlorate) (Molecular Probes, Thermo Fisher Scientific)[87] ...
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bioRxiv - Neuroscience 2020Quote: The tips of laminar implants were stained before surgery with the orange-red fluorescent® membrane stain DiI (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate; Thermo Fisher Scientific, Waltham, MA, USA) for later histological assessment of the electrode position ...
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bioRxiv - Neuroscience 2020Quote: ... to identify fluorescent particles on confocal stacks using Drosophila brains stained with the lipid droplet dye BODIPY 493/503 (Molecular Probes, D-3922) or labelled with the glial specific marker Repo (immunlocalization of glial nuclei) ...
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bioRxiv - Bioengineering 2021Quote: The spatial resolution of CHAMP was measured by imaging 500-nm-diameter fluorescent beads (B500, excitation/emission: 365/445 nm, Thermo Fisher Scientific Inc.) (Supplementary Fig ...
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bioRxiv - Neuroscience 2021Quote: ... patch pipettes were filled with the same cesium-base solution described above supplemented with 50 µM of the red fluorescent indicator Rhod2 (R14220, Invitrogen™, Thermofisher Scientific). The Ca2+ dye was excited with a 543 nm Helium Neon Laser system (Melles Griot 05-LGR-193-381 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with 200 nM of a solution of SYBR Green I (SGI) fluorescent nucleic acid stain (Molecular Probes, Life Technologies Corp.) for 45-60 min in the dark at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... in many cases covered with a fluorescent dye to enable post-hoc track identification (DiI, DiR or DiO, Thermo Fisher Scientific, MI, USA), was lowered slowly into the brain at a 30° angle (coronal orientation ...
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bioRxiv - Molecular Biology 2020Quote: ... Both genomic DNA and cDNAs were analyzed on DT96 real-time DNA amplifier (DNA-Technology) using SYTO™ 13 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific). RT-qPCR values were normalized to the rp49 mRNA and genomic DNA qPCR values were normalized to rp49 genomic qPCR ...
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bioRxiv - Microbiology 2020Quote: ... 1.3 µL of 1 mg/mL stock of the lipophilic fluorescent dye BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, 493/503, Molecular Probes, ThermoFisher Scientific, USA) per sample was added to stain for neutral lipids (TAG) ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were harvested and fixed with 3 % PFA for 15 minutes and then stained with 2 μM of CellTracker™ fluorescent probes (ThermoFisher Scientific #C34552). Z-stacks were taken using a Leica TCS SP5 Matrix confocal microscope with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated with rabbit anti-tyrosine hydroxylase antibodies (TH; AB152, MilliporeSigma, MO) at 4°C overnight and then with fluorescent secondary antibodies (A-11012, Thermo Fisher Scientific, MA) at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... MCF-7 breast cancer cells were cultured and stained with a live fluorescent dye (CellTracker™ Red CMTPX dye, Invitrogen™, Carlsbad, USA). WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX ...
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bioRxiv - Neuroscience 2021Quote: ... syntaxin molecules were labeled at the N-terminal primary amino group (-NH2) of the proteins using fluorescent dye Alexa 488 N-hydroxysuccinimide (NHS) (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The washed cells were then resuspended in 1mL of PBS and stained with SYTO 17 red fluorescent nucleic acid stain (Molecular Probes, Inc. OR) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Pathology 2021Quote: ... Plasma membrane patches and potential contaminations by remaining cortical ER were stained with red fluorescent CellMask™ Deep Red (CMDR) and ER-tracker™ Blue-white DPX (both from ThermoFisher Scientific), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... The quantity of DNA was estimated by comparing the fluorescent yield of the samples with a series of lambda (λ) DNA standards (Invitrogen, Carlsbad, CA) at varying known concentrations ...
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bioRxiv - Bioengineering 2020Quote: ... Nuclei blue fluorescent staining was done using Invitrogen™ Molecular Probes™ NucBlue Fixed Cell ReadyProbes™ Reagent (Fisher Scientific, Waltham, MA, USA) incubated for 5 min followed by rinsing with tap water ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescent G-actin was prepared by labelling G-actin with AlexaFluor 488 carboxylic acid succimidyl ester (Invitrogen, through Thermo Fisher Scientific, Cat. # A20000) following Ref ...
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bioRxiv - Neuroscience 2022Quote: ... The injections were either a fluorescent dextran amine or a recombinant cholera toxin subunit B tracer: (Dextran-405; CTB-488, CTB-594, CTB-647, Invitrogen #C22841, #C34777, #C34778). We chose our injection volume based on the size ...
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bioRxiv - Molecular Biology 2022Quote: ... All sections were mounted with 50μl of ProLong Gold Antifade mounting media containing a fluorescent nucleic acid dye 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, P36931) before imaging ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Quantification of transcript was done by qRT-PCR with fluorescent-labelled nucleotide substrate (TB Green™ Premix Ex Taq™ II, Takara or PowerUp SYBR Green Master Mix, Thermo Scientific) as described previously (Shigeyama et al ...
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bioRxiv - Neuroscience 2024Quote: ... Mitochondria in cultured RGCs were labeled by the addition of a red fluorescent mitochondrial dye (100 nM final concentration; MitoTracker Red CMXRos; Invitrogen, Carlsbad, CA, USA) to the cultures and were maintained for 20 min in a CO2 incubator ...
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bioRxiv - Cell Biology 2024Quote: ... followed by fluorescent visualization with donkey anti-mouse or anti-rabbit Alexa fluor-488 or -555 (A31572, A21202, Thermo Fisher Scientific, MA, USA). The stained coverslip was further incubated with DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent intensity (Ex/Em=485/523nm) of supernatants and cell lysates was measured using Varioskan LUX multimode microplate reader (Thermo Fisher Scientific, VL0000D0). Percentage of cholesterol efflux was quantified as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent images were captured and cell surface area of 200 α-actinin+ cardiomyocytes per sample was automatically measured using ArrayScan (Thermo Fisher Scientific) [43].
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bioRxiv - Molecular Biology 2023Quote: The ΔΨm of wild-type and TbFUT1 cKO cells grown in permissive and non-permissive conditions was estimated using the cell permeant red-fluorescent dye tetramethyl rhodamine (TMRE, Thermo Fisher Scientific T669). Fluorescence intensity is proportionally dependent on the ΔΨm values ...
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bioRxiv - Neuroscience 2023Quote: ... anterograde labeling of retinal ganglion cell projections was conducted using intraocular injection of the fluorescent neuronal tracers CTB-AlexaFluor 555 and 647 (#C34776 and #C34778, Thermo Fisher, Waltham, Massachusetts), iDISCO+ brain clearing and 3D imaging with lightsheet microscopy (2X acquisition magnified 0,8X ...
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bioRxiv - Cell Biology 2023Quote: ... The exact parameters for the transformation were manually determined by achieving best visible overlap for a dual-channel dataset obtained by imaging 200 nm diameter fluorescent beads (T7280, TetraSpeck™, Thermo Fisher Scientific) embedded in agarose.
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bioRxiv - Cancer Biology 2023Quote: ... The EdU was visualized by click chemistry with azide-linked fluorescent dyes as described in the manufacturer’s protocol (Thermo Fisher, #C10340 and #C10641). Cells were then twice washed with PBS and blocked with 3% BSA for 1 h at room temperature to prepare for further immunostaining.
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bioRxiv - Cell Biology 2023Quote: ... using an automated simultaneous pipettor (analytikjena CyBio® Well vario) and incubated with the fluorescent calcium indicator Fluo4 (2 µM, Life Technologies, #F14202) in DMEM without supplementation for 1 h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: Cells grown to mid-log phase were fluorescently labeled using 20 μM SYTO® BC Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific) in their growth medium ...
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bioRxiv - Neuroscience 2023Quote: ... Sections in the vicinity of electrodes were mounted onto 0.1% gelatin-subbed slides and stained with NeuroTrace 530/615 Red Fluorescent Nissl Stain (N21482, ThermoFisher Scientific, Waltham, MA, USA) to confirm proper placement (Fig ...
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bioRxiv - Biophysics 2023Quote: ... a 1:625 stock dilution of 0.1 μm diameter TetraSpeck Microspheres (TetraSpeck™ Microspheres, 0.1 μm, fluorescent blue/green/orange/dark red, Thermo Fisher, Catalogue number T7279) was added ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1000 rpm for 4 min and resuspended in PBS plus LIVE/DEAD™ Fixable Dead Cell Stain Near-IR fluorescent reactive dye (Thermo Fisher; L34976) and incubated for 30 min protected from light ...
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bioRxiv - Neuroscience 2024Quote: ... Sections contaiing the electrode sites were then mounted on histology slides and labeled with a green fluorescent Nissl stain (Invitrogen NeuroTrace, catalog# N21480). Slides were then imaged at 2x using an automated fluorescent microscope (Keyence BZ-X800) ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Neuroscience 2024Quote: ... The electrode array was stained with a fluorescent dye (cell tracker CM-Dil dye, red excitation/emission spectra: 553/570 nm; ThermoFisher Scientific, MA, USA) just before it was inserted into the brain ...
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bioRxiv - Bioengineering 2022Quote: Rheological characterization of the jammed microgel ink and PAM scaffolds was performed using parallel plates geometry (20 mm diameter, 1 mm gap) mounted on strain-controlled rheometers (Thermo Scientific HAAKE MARS 40/60 Rheometer). Jammed microgels were prepared in the same way and molded to an identical geometry to the plates ...
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bioRxiv - Immunology 2023Quote: ... the plasmid was altered via site-directed mutagenesis to match the S sequence to the corresponding variant sequence as previously described.39,67 A pre-titrated dose of pseudovirus was incubated with eight serial 5-fold dilutions of serum samples (1:20 start dilution) in duplicate in 96-well 384-well flat-bottom tissue culture plates (Thermo Fisher, cat. no. 12-565-344) for 1 hr at 37°C before adding 293T/ACE2 cells ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using SYBR green Supermix (OZYME) in an ABI7500 Prism SDS Real-Time PCR Detection System (Applied Biosystems). The mRNA content was normalized to β-actin mRNA and quantified using the 2-ΔΔCt method ...