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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for Recombinant Human HUS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... we incubated 2-5μg sheared DNA with equal volume of DynabeadsTM Protein A and Protein G (ThermoFisher Scientific Inc.). ChIP-seq libraries were prepared with a NEBNext DNA Ultra II kit following manufacturer recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... using the Qbit 4 fluorometer (with the Broad range protein assay kit Qubit Protein BR Assay, from ThermoFisher Scientific), and sugar concentration using the refractometer (refractometer RBR32-ATC) ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA-protein-antibody complex was captured using protein A/G magnetic beads (Thermo Fisher Scientific, 10002D and 10004D). Magnetic beads were washed with low salt buffer (1X PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... SDS-PAGE was performed using 20 – 25 μg protein from each sample with a prestained protein ladder (ThermoFisher, 26616). Western blotting was performed using antibodies against KMT5C (AbClonal ...
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bioRxiv - Molecular Biology 2023Quote: ... containing proteins were collected in a fresh tube and protein quantification was done using Pierce BCA assay (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein was measured using Pierce BCA Protein Assay Kit according to the manufacturer’s microplate protocol (Thermo Fisher 23225). A final concentration of 1X Laemmli sample buffer (Bio-Rad 161-0747 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of protein concentration was performed using the Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific, IL, USA). For WB analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein content was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Dreieich, Germany, #23227) with 25 μL of supernatant mixed with 200 μL of reagent ...
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bioRxiv - Physiology 2023Quote: ... The respective protein concentration was measured using the PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA, USA). Mitochondria-rich fraction (10 µg of protein ...
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bioRxiv - Cell Biology 2023Quote: Lysates containing 25 µg of protein were electrophoresed on a NuPage 4%-12% Bis-Tris Protein gel (Thermofisher Scientific). Proteins were transferred to a PVDF membrane (0.2 µm ...
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bioRxiv - Biochemistry 2023Quote: ... The protein concentration of each AF sample was measured using a Pierce BCA Protein Assay kit (Thermo scientific 23227) and then normalized to equal concentrations for downstream analysis.
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bioRxiv - Cell Biology 2023Quote: ... and protein concentration of the supernatant was determined by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific; 23225). Spastin protein was resolved on 8% SDS-PAGE gels transferred to Immobilon®-FL PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated and the total protein amount was determined with a BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and protein expression was induced by methanol in BMMY medium following the description in Pichia protein expression handbook (Invitrogen). SCP8 protein was purified by affinity chromatography with a His-trap column (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Lysates were collected and protein concentration was measured using the Pierce BCA Protein Assay Kit assay (Thermo Scientific #23225) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The total protein content was analyzed by biochinonic acid-assay (Pierce™ BCA Protein Assay Kit, Thermofisher Scientific, USA). The total protein content of the NTHi lysate was adjusted to 2.5 mg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Total protein was quantified in placenta/trophoblast homogenates using the Pierce BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA). T3 and T4 concentrations was normalized to total protein for each sample.
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the clarified supernatant was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were then loaded onto a NativePAGE Novex 4-16% Bis-Tris Protein Gel (Life Technologies). A wet blotting method was used to transfer the proteins onto a PVDF membrane (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: The protein concentration of EV enriched fractions was quantified by Pierce BCA Protein Assay Kit (Thermo Scientific, Waltham, MA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: Protein concentrations in cell lysates were determined by Pierce BCA Protein Assay according to the manufacturer’s protocol (Thermo Scientific). Aliquots of cell lysates containing 50 µg total protein were snap frozen ...
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bioRxiv - Cell Biology 2023Quote: Protein synthesis was monitored with L-azidohomoalanine (AHA) using a Click-iT™ Nascent Protein Synthesis kit (Thermo Scientific) according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected and protein quantification was done with BCA reagent (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). 20 μg of each sample was separated on 8% SDS-PAGE followed by transfer onto a nitrocellulose membrane ...
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bioRxiv - Immunology 2023Quote: ... Proteins (10 μg) were subjected to SDS-PAGE on Bolt Bis-Tris mini protein gels (Invitrogen, Waltham, Massachusetts, USA) and transferred to nitrocellulose (GE HealthCare ...
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bioRxiv - Immunology 2023Quote: Cells were lysed in Laemmli buffer and the protein content quantified using EZQ™ protein quantification kit (Thermo Fisher). Equal protein amounts were loaded and separated on 4%– 15% pre-cast gels (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentration was quantified in a plate reader using a Pierce BCA protein quantification kit (Thermo Fisher). For each condition ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was collected and total protein was quantified using Pierce BCA protein assay kit (Cat# 23225, Thermo Fisher Scientific). A 96 well plate was coated with 2 µg/mL spike antibody overnight at 4°C (Cat# 41050-D003 ...
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bioRxiv - Microbiology 2023Quote: ... separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor) on 4–12% Bis-Tris Gels (Invitrogen) at 150 V for 90 min ...
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bioRxiv - Immunology 2023Quote: ... concentration of whole proteins in the lysate was determined by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and concentration of IFNγ (pg/mg total protein ...
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bioRxiv - Biochemistry 2023Quote: ... Purified and concentrated EGFP_Nanobody protein was immobilized onto agarose beads (20 mg protein per column) using AminoLink Plus Immobilization Kit (ThermoFisher), following manufacturer’s instructions in PBS pH 7.5 coupling buffer.
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bioRxiv - Evolutionary Biology 2023Quote: ... Both measurements had CV% < 10 and are expressed per mg of protein (measured via BCA protein assay, ThermoFisher Scientific), following Espin et al ...
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bioRxiv - Biochemistry 2023Quote: ... The total protein concentration from the lysate was measured by Rapid Gold BCA protein assay kit (Thermo Scientific A53225) to normalize the amount of total protein in each sample ...
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bioRxiv - Biochemistry 2023Quote: ... The protein content of the cleared lysates were quantified using Pierce Rapid Gold BCA Protein Kit (Thermo Scientific, A53226) and separated by Bio-Rad TGX stain-free gel ...
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bioRxiv - Plant Biology 2022Quote: ... The gel was re-used for protein quantification using SYPRO Ruby Protein Gel Stain (Themo Fisher Scientific, Loughborough, UK) following manufacturer’s instructions and imaged using a UV transilluminator (Ultra-Violet Products Ltd ...
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bioRxiv - Cancer Biology 2023Quote: ... The total amount of protein was quantified using the Qubit Protein Assay Kit and a Qubit Fluorometer (Thermo Fisher). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... 20 mg total protein in 200 ml NLB was precleared with 10 ml protein A Dynabeads (Thermo Fisher Scientific) for 20 min at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Protein content in cell lysates and EVs was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Between 5 and 20 μg of total protein (kept consistent for individual experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of total protein were loaded on NovexTM 10% Zymogram Plus (Gelatin) Protein Gels (Thermo Fisher Scientific: ZY00102BOX) using 2x non-reducing sample buffer (1M Tris-HCl pH 6,8 (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration in the samples was quantified with the Pierce™ BCA Protein Assay Kit (23227, Thermo Scientific) and equal amount of proteins were loaded on the gel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations in the lysates were measured with the Pierce BCA Protein Assay Kit (Thermo Scientific™, CAT: 23227). Lysate mixed (10-25 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein was measured using Pierce BCA Protein Assay Kit according to the manufacture’s microplate protocol (Thermo Fisher 23225). 1X Laemmli sample buffer (Bio-Rad 161-0747 ...
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bioRxiv - Genomics 2022Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop ND1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Protein samples were prepared from 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentrations of solubilized protein precipitates were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Bremen, Germany). For LC-MS/MS ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected and the protein concentration was quantified using bicinchoninic acid protein assay (BCA assay; Pierce, Thermo Scientific). 50 μg of protein lysate was denatured and were run on a 4-12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein concentration was determined using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher #53226). 30 μg of total protein sample was mixed with Laemmli buffer (180 mM DTT (Sigma #D9779) ...
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bioRxiv - Genomics 2023Quote: ... Protein quantification for each fraction was done using manufacturer’s protocol for Pierce 660nm protein assay reagent (Thermo Scientific, USA). Each Fraction was subjected to iCLIP protocol as described in Huppertz et al 201441 using rabbit-CLAMP antibody to immuniprecipitate bound RNAs which were extracted using proteinase K and phenol:chloroform ...
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bioRxiv - Biochemistry 2023Quote: ... Protein bands were visualised by Coomassie blue staining using QuickBlue Protein Stain (LuBio, Zürich, Switzerland) and accompanied by either a SeeBlueTM Plus2 Pre-stained Protein Ladder (Invitrogen) or a PageRuler Prestained Protein Ladder (Thermo Scientific ...