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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for Recombinant Human HUS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The total extravesicular protein content was quantified using the micro bicinchonic acid protein assay (BCA) (#23235, Thermo Fisher scientific).
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was determined using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... and the total protein content was determined photometrically with the Pierce bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... aliquots of the supernatant were subjected to protein quantification with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and SDS-PAGE using a TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentrations of the purification fractions were estimated using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturers protocol.
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bioRxiv - Systems Biology 2022Quote: ... The protein concentration of the samples was measured using a Pierce BCA Protein Assay Kit (Thermo Scientific, Cat#23225). To determine the proteasome activity ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Cancer Biology 2022Quote: ... and normalized to protein concentration measurement carried out using Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. 23227).
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bioRxiv - Developmental Biology 2022Quote: ... The whole sperm protein extract thus obtained was quantified using Pierce™ 660nm protein assay reagent (Thermo Scientific™) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... 50µg of protein sample along with 5µl of Page RulerTM pre-stained protein ladder (Cat no. 26617, Thermo Scientific), was loaded and gel was run initially at 50 volts until the tracking dye crosses the stacking gel ...
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bioRxiv - Microbiology 2019Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as a standard ...
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bioRxiv - Genetics 2019Quote: ... Biotinylated surface proteins were recovered by incubating 200 μg of total protein with streptavidin-agarose beads (20347, Thermo Fisher) and eluting in Laemmli sample buffer ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Microbiology 2020Quote: ... The supernate was collected and protein was quantified by BCA assay (Pierce BCA Protein Assay Kit ThermoFisher Scientific, 23225). To measure ACE2 expression in transfected cells ...
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bioRxiv - Neuroscience 2019Quote: ... Membrane proteins were extracted using the Mem-PER Plus Membrane Protein Extraction Kit (Cat# 89842; ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions using a mixture of protease and phosphatase inhibitors (10 µl/ml ...
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bioRxiv - Physiology 2019Quote: ... 15 μl of homogenate was used for protein measurement using a Pierce BCA protein Assay Kit (#10678484, Fisher Scientific) and a standard curve was calculated with a series of Albumin dilutions (0.025 ...
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bioRxiv - Molecular Biology 2020Quote: ... The ladder used for proteins larger than 240 kDa was 12 µl HiMark Pre-Stained Protein Standard (Thermo Scientific). The ladder used for proteins smaller than 240 kDa was 2.5 µl BLUltra Prestained Protein Ladder (GeneDireX) ...
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bioRxiv - Molecular Biology 2020Quote: ... CSDE1 protein was then captured by incubation with an affinity purified CSDE1 antibody conjugated to protein A Dynabeads (Invitrogen) for 4 hours at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2020Quote: ... Ten μg of protein per sample was loaded on NovexTM 10-20% Tris-Glycine protein gels (Thermo Fisher Scientific) and transferred onto iBlot2 Transfer Stacks PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration of each qEV fraction was estimated by BCA protein assay according to the manufacturer’s protocol (ThermoFisher Scientific). Technical replicates by serial dilution were performed.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total BAL protein was quantified with the Qubit Total Protein Quantification kit and the Qubit 2.0 fluorometer (Thermo Scientific). BAL cytokines were profiled using a pre-mixed MILLIPLEX protein immunoassay (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentrations of the cell lysates were determined with a BCA protein assay kit (Thermo Fisher Scientific, USA). The cell lysates were pre-cleaned by an off-target control antibody and aliquoted ...
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bioRxiv - Neuroscience 2019Quote: ... Protein content was quantified using the Pierce™ BCA Protein Assay kit according to manufacturer protocols (Thermo Fisher Scientific). 20 μg of protein lysate was run on 4-20% Mini-PROTEAN TGX Precast Gel (BioRad) ...
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bioRxiv - Developmental Biology 2019Quote: Protein concentrations for individual samples were determined using a BCA protein assay following the manufacturer’s protocol (Thermo Scientific Pierce). 50 μg of protein were labeled with Alexa Fluor 555 and all reactions were quenched using 2M Tris (pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein quantification was performed using the protocol and supplies provided by the BCA Protein Assay Kit (Thermo Scientific Pierce). Samples of BSA protein in PBS ranging in concentration from 25 ug/ml to 2000 ug/ml were used to create the standard concentration curve ...
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bioRxiv - Genetics 2020Quote: ... the supernatant was used to assess protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, #23225) before subsequent analysis ...
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bioRxiv - Genomics 2021Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants from each filter were transferred into eppendorfs and protein concentrations were determined using Pierce Protein Quantitation Assay (ThermoFisher). Protein concentrations across samples were normalized to 4 mg/ml using 50mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
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bioRxiv - Systems Biology 2019Quote: ... The protein concentration in the cell lysate was quantified with a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, UK) using the provided albumin as standard ...
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bioRxiv - Synthetic Biology 2019Quote: ... The total protein concentration of MCP samples was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate protein concentration was determined using Pierce BCA protein assay kit (Cat#23225; Lot no. QI223168; ThermoFisher Scientific). 50 μg of cell lysate was loaded onto a 4-12% gradient gel and ran at 165 V constant for 45-60 min in 1X MOPS-SDS buffer (NuPAGE ...
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bioRxiv - Plant Biology 2019Quote: ... About 20 µg of total soluble proteins of each sample was loaded to 3-12% Bis-Tris 1.0 mm Invitrogen NativePAGETM protein gels from ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... RNA binding proteins were isolated by the means of Pierce™ Magnetic RNA-Protein Pull-Down Kit (ThermoFisher, #20164). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were denatured at 95°C for 10min and protein quantity was measured using Pierce 660nm Protein Assay (ThermoFisher). Protein extracts (10µg ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was measured by Qubit Protein Assay Kit on the Qubit fluorometer (Thermo Fisher scientific, Waltham, MA, USA). PO assay was performed by a modified Alaux protocol (Alaux et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... The concentration of total protein was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated electrophoretically on an SDS-PAGE gel ...
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bioRxiv - Cell Biology 2021Quote: Total protein was extracted using RIPR lysis buffer (KeyGEN, China) and quantified using the BCA protein assay (Thermo Scientific). The proteins of tissue samples were resolved by 10% SDS-PAGE and transferred to the PVDF membrane (0.2 µm pore size ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration was measured by Pierce BCA protein assay kit (Cat: 23228 from Thermo Fisher Scientific Inc, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration in each sample was determined using BCA protein assay kit (Cat no. Thermo Fisher Scientific, Massachusetts, USA) and equal amount of proteins were incubated with “MyOne Streptavidin C1” magnetic beads (Cat no ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... Env proteins bound to magnetic beads were separated from unbound proteins using a DynaMag 15 magnet (Life Technologies, Inc.). Env protein bound beads were further incubated with blocking buffer (PBS [pH 7.4] ...
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bioRxiv - Microbiology 2021Quote: ... Final absolute nucleotide concentrations were normalized to total protein concentrations using the Pierce 660 nm Protein Assay (Thermo Scientific). Extraction experiments were repeated on separate days using two biological replicate cultures for each strain and temperature condition ...
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bioRxiv - Neuroscience 2020Quote: The cell surface proteins of hippocampus were isolated using Pierce cell surface protein isolation kit (Pierce, Thermo Scientific, MA). Biotinylated proteins bound to NeutrAvidin beads (bound fraction ...
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bioRxiv - Neuroscience 2022Quote: ... An aliquot from each supernatant was used to quantify total protein using the BCA Protein Assay Kit (ThermoFisher Scientific). The rest of the supernatant was filtered using an amicon-ultra© centrifuge filter with a cut-off of 3KD filter (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of each sample were determined using the Pierce™ BCA Protein Assay Kit (#23225, Thermo Fisher Scientific) according to the manufacturer’s protocol using the Photometer Tecan Infinite®200M (Tecan).