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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were 1:5000 anti-rat Alexa488 and anti-mouse Alexa594 conjugated antibodies (Cat #A32740 Invitrogen, CA, USA). The sections were mounted using ProLong Gold with DAPI (Cat #S36964 Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoblots were developed by HRP-conjugated secondary antibodies (anti-mouse or anti-rabbit, #31430 or #31460, 1:5,000; Thermo Fisher) followed by ECL plus exposure ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed and probed with horseradish-peroxidase-conjugated donkey anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (SA1-100, Invitrogen; 31438, Invitrogen) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were activated by co-incubation with anti-CD3/anti-CD28 antibody conjugated Dynabeads™ (Gibco, Catalogue No. 11141D) in X-VIVO 15 medium (Lonza ...
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bioRxiv - Neuroscience 2022Quote: ... for ASO: anti-rabbit Alexa Fluor Plus 647, A32795 ThermoFisher Scientific; for NeuN: anti-guinea pig Alexa Fluor 555, A21435 Invitrogen or anti-chicken Alexa Fluor 594 ...
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bioRxiv - Neuroscience 2023Quote: ... with TBST and incubated for 2 hours with horseradish peroxidase coupled secondary goat anti-rabbit or anti-mouse IgG (1:5000, Invitrogen) in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed and incubated with Alexa Fluor 594 donkey anti-rabbit antibody and Alexa Fluor 488 anti-guinea pig (ThermoFisher) at 1:2000 in 0.3% Triton in 0.1 M PB ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed and incubated with secondary antibodies (anti-mouse Cat. # A-21200 and anti-rabbit Cat. # A-11012; Invitrogen) at a dilution of 1:1000 for 1 h/20°C.
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were then incubated with anti-rabbit or anti-mouse antibodies (1:1000; Cat #. A-21200 and Cat #. A-11012; Invitrogen) and fixed in 1% agarose using Prolong Gold Antifade mounting solution containing DAPI (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... samples (excluded those stained with anti-mouse CD45.1-AlexaFluor647) were incubated with a secondary goat anti-rabbit antibody conjugated to Alexa Fluor 647 (ThermoFisher Scientific) diluted 1:300 in 2% BSA in PBS for 1 hour at RT ...
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bioRxiv - Microbiology 2022Quote: ... Anti-HDAg was detected using donkey anti-rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1:500; Invitrogen, A21206), and Anti-AQP5 ...
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bioRxiv - Microbiology 2023Quote: ... and Spo0A-3xFLAG was detected using mouse anti-Spo0A antibody.30 Goat anti-mouse IgG conjugated with Alexa 488 (Invitrogen) was used as a secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and the secondary antibodies (goat anti-rabbit IgG Alexa Fluor 647, Invitrogen, A-21245; goat anti-rabbit IgG Alexa Fluor 568, Invitrogen, A-11036 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa-conjugated secondary antibodies (1:1000, goat anti-rabbit IgG Alexa Fluor 647, Invitrogen, A21245; goat anti-rabbit IgG Alexa Fluor 568, Invitrogen, A-11036 ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked in 5% BSA in PBS and rat-anti-DILP2 was incubated overnight at 4°C (Geminard et al., 2009) followed by Alexa488 anti-rat-secondary antibodies (Invitrogen). Tissues were washed and mounted in Vectashield with DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with secondary antibodies (anti-rabbit conjugated with Alexa Flour 488, anti-rabbit conjugated with Alexa Flour 546, anti-mouse conjugated with Alexa Flour 546; Invitrogen) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405 ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... After overnight incubation samples were washed in PBS and incubated with Alexa Fluor anti-mouse 488 and anti-rabbit 546 secondary antibodies (Invitrogen) diluted in 1% blocking buffer at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse Alexa FluorTM 594 (A-11004) and donkey anti-rabbit Alexa FluorTM 488 (A-21206) were purchased from ThermoFisher Scientific and used at 1:400 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were used at 1 μg mL-1 for goat anti-rabbit and sheep anti-mouse linked horseradish peroxidase (Invitrogen). Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... CRL-1772) were maintained in DMEM (WELGENE, Korea) supplemented with 10% fetal bovine serum (FBS) (WELGENE) and 1% Anti-anti (Gibco) at 37 °C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... blotted onto nitrocellulose, and probed with primary antibody anti-XePP1 (Santa-Cruz Biotechnology, 1:1500) or anti-HA (1:1500, Invitrogen) followed by anti-mouse or anti-rabbit secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were then incubated for 2 hours at RT with a 1:200 dilution of the respective secondary antibody: goat anti-mouse or goat anti-rabbit conjugated to Alexa Fluor-488 (Invitrogen). Membranes were then washed three times in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were rinsed 3 x 10 min in PBS-Tween20 prior to incubation with appropriate secondary antibody (anti-mouse DyLight 800 and anti-rabbit DyLight 800 conjugated antibodies SA5-35521 or SA5-35571 from Invitrogen) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary HRP-conjugated anti-mouse and anti-rabbit used for western blot were from Invitrogen (31430 and 31460, respectively). The fluorescent goat anti-rabbit secondary conjugated with Alexa 633 and a goat anti-mouse secondary antibody conjugated with Alexa 594 were acquired from Thermo Fisher Scientific (A-21070 and A-11032 ...
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bioRxiv - Pathology 2023Quote: ... The organ of Corti was stained with anti-prestin (Zheng et al., 2005) followed by goat anti-rabbit Alexa Fluor 488 secondary antibody (ThermoFisher). Images were taken using a Leica DM IRB fluorescence microscope or Keyence BZ-X800 for OHC counts as previously described (Takahashi et al. ...
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bioRxiv - Genomics 2023Quote: ... Antibodies for immunofluorescence staining were goat anti-mouse Alexa Fluor 488 (A11029, 1:300) and goat anti-rabbit Alexa Fluor 555 (A21428, 1:300) from Invitrogen.
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bioRxiv - Bioengineering 2023Quote: ... the sections were washed and a mixture of secondary antibodies composed of Alexa Fluor 568 rabbit anti–goat IgG and Alexa Fluor 488 goat anti–rabbit IgG (Invitrogen) were applied to labeled CD11c and EGFP ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with secondary antibodies (AlexaFluor 488 goat anti-chicken [Invitrogen, A11039] and Alexa Fluor Plus 647 goat anti-rabbit [Invitrogen, A32733] ...
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bioRxiv - Immunology 2022Quote: ... DAPI and secondary antibody staining was performed at room temperature for 30 minutes using anti-mouse or anti-rabbit Alexa Fluor 647 (Invitrogen) at the above indicated dilutions ...
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bioRxiv - Bioengineering 2023Quote: ... Germany) and activated with mouse anti-CD3 (5ug/ml) and mouse anti-CD28 (5ug/ml) (Gibco, Billings, MT, USA, 11132D) antibodies for 48-72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... After several washes with PBS cover slips were incubated with secondary antibodies Alexa fluor goat anti-mouse 488 and anti-rabbit 594 (Invitrogen). DAPI was used to stain nuclei ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated for 45 minutes in secondary antibody solutions with AF647 secondary antibodies (anti-mouse, 1:1000, A31571, Invitrogen or anti-rabbit, 1:1000, A21245, Invitrogen) or Alexa Fluor 555 secondary antibodies (anti-rabbit ...
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bioRxiv - Biochemistry 2022Quote: ... Phosphotyrosine production was assessed via anti-phosphotyrosine Western or dot blotting using primary mouse anti-phosphotyrosine antibody (PY20, Thermo Scientific) followed by goat anti-mouse HRP conjugated secondary antibody (GOXMO HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (H+L) or HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (Invitrogen). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... the following secondary antibodies were used: goat anti-rabbit IgG labelled with Alexa 488 and donkey anti-mouse labelled with Alexa 555 (1:1000, Invitrogen) 1h at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor Plus 488-conjugated goat anti-mouse IgG (A32723) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (A32740) were purchased from Invitrogen. Alexa Fluor Plus 488-conjugated donkey anti-goat IgG (bs-0294D-AF488 ...
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bioRxiv - Genomics 2023Quote: ... These cells were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression (antibody amount required determined by prior experimentation) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize Aurora kinase C, we used rabbit anti-mAurC (1:500, FORTIS) and Alexa-Fluor 568 donkey anti-rabbit IgG (1:200, Invitrogen). For H2AT120ph ...
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bioRxiv - Genomics 2023Quote: ... PBMCs were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression on CD4+ T cells (antibody amount required determined by prior experimentation) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The secondary antibodies were diluted to 1:250 (Goat anti-rabbit IgG Cy5; Invitrogen A10523; goat anti-mouse IgG AlexaFluor 488, Invitrogen A11029 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594, 1:200, Invitrogen). The cells were treated with antifade mountant (SlowFade Diamond ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with 3 % BSA at 37 °C for 1 h followed by incubation with the indicated antibody for 2 h at 4 °C and then detected by Alexa-633-conjugated anti-mouse or Alexa-488 conjugated anti-rabbit secondary antibody for 30 min (Invitrogen). Images were taken using Zeiss microscope and image analysis was done using the Zeiss LSM or ZEN software tools.
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bioRxiv - Microbiology 2023Quote: ... ten-fold dilutions of tissue homogenates or serum were made in DMEM supplemented with 1% Antibiotic-Antimycotic (Anti-Anti, Invitrogen). Monolayers were infected with 50 μl of the dilutions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated with fluorescently labelled secondary antibodies (1:1,000 G-anti-Rb-AlexaFluor-488, G-anti-M-AlexaFluor-555, ThermoFisher Scientific) and DAPI in PBS+ for 1.5 hr at room temperature before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Sections were incubated in secondary antibodies (1:1000 Alexa Fluor 488 anti-rabbit IgG [Invitrogen]; 1:1000 Alexa Fluor 488 anti-mouse IgG [Invitrogen] ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...