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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Microbiology 2022Quote: ... and washed in PBS two additional times before adding secondary anti-goat Cy5 (1:2500, 072-02-13-06, KPL) or anti-mouse Alexa Fluor 647 (1:2500, A32728, Invitrogen) and incubated at 4°C in the dark for 30 mins ...
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bioRxiv - Microbiology 2022Quote: ... Slides were incubated with primary antibodies followed by Alexa Fluor 488-conjugated goat anti-mouse secondary antibody or Alexa Fluor 594-conjugated goat anti-rabbit secondary antibody (diluted 1:400) (Invitrogen). The nuclei were stained with DAPI (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in PBS-Tween-20 containing 5% BSA for 1 h and incubated overnight at 4 °C with rabbit polyclonal anti-MIF antibody Ka565 (Bernhagen et al., 2007) or rabbit polyclonal anti-PF4V1 IgG PA5-21944 (Invitrogen) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies were donkey anti-rabbit Alexa Fluor 594 and donkey anti-goat Alexa Fluor 488 (Life Technologies, Victoria, Australia) diluted in Dako REAL Antibody Diluent and incubated in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were then washed 4 x 2 h in PBT and incubated overnight at 4°C in PBT supplemented with goat anti-mouse and goat anti-rabbit secondary antibodies coupled to Alexa Fluor 488 and 568 (Invitrogen). Embryos were then washed 4 x 2 h in PBT and gradually dehydrated in methanol ...
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bioRxiv - Neuroscience 2021Quote: ... SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649; donkey anti-guinea pig-conjugated Alexa Fluor 405 [1:1000, ThermoFisher]) diluted in 0.2% Triton X-100 blocking solution ...
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bioRxiv - Microbiology 2021Quote: ... Blots were then washed extensively and incubated with HRP-conjugated goat anti-mouse or anti-rabbit IgG secondary antibodies (Invitrogen). Blots were developed using the SuperSignal West Pico PLUS chemiluminescent substrate kit (Thermo ...
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bioRxiv - Neuroscience 2019Quote: ... Afterwards the preparations were washed with PBS 8 x 30 min and then incubated with secondary antibodies (donkey anti-chicken Cy2, 1:400, Dianova, cat# 703-505-155; donkey anti-rat Cy3, 1:400, Invitrogen, A-21209 ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were goat anti-mouse IgG conjugated with Alexa 488 or 546 and goat anti-rabbit IgG conjugated with Alexa 546 (Invitrogen). Alexa 633–phalloidin at 1:300 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed 3 × 30 min and incubated with secondary antibodies (Donkey anti-mouse 405, 1:500 [invitrogen] and Donkey anti-guinea pig 647 [Invitrogen]). After the last round of washing (3 × 30 min in PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed twice with PBS and incubated with fluorescent secondary antibodies (Anti-Rat Goat Alexa Fluor 488 [A-11070], Anti-Mouse Goat Alexa Fluor 647, Thermofisher) in PBSA-T for one hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec) and an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen). Following 3 washes with PBS containing 0.5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-goat Alexa Fluor 488 (1:1000) and donkey anti-rabbit Alexa Fluor 594 (1:500) respectively (both from Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... and secondary antibodies (anti-rabbit Alexa Fluor 594, Thermo Fisher Scientific A-11037; anti-goat Alexa Fluor 594, Thermo Fisher Scientific A-11058 ...
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bioRxiv - Bioengineering 2020Quote: ... SAG1 was detected with rabbit anti-SAG1 polyclonal antibody and V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen). Primary antibodies were detected with goat polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... Coverslips were then washed 3 times with PBS-saponin and stained with anti-mouse Alexa Fluor 488 and anti-rabbit Alexa Fluor 568-conjugated secondary antibodies (Invitrogen), and Hoechst for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour incubation period at room temperature with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]) made in PBST-NGS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then washed in 0.1% Tween/PBS and incubated with a donkey anti-mouse or anti-rabbit HRP coupled antibody (Life Technologies) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 hour incubation with Alexa Fluor 647-conjugated secondary antibody (donkey anti guinea pig, 1:1000) or Cy3-conjugated secondary antibody (goat anti mouse,1:1000, Invitrogen) respectively.
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bioRxiv - Microbiology 2021Quote: ADAP1CRISPR and CtrlCRISPR TM were left resting or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... Furin bands were detected using rabbit anti-furin polyclonal at 1:1000 and Goat anti-rabbit HRP (Invitrogen, 1:5000) was used as secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated with monoclonal mouse anti-3D8 scFv antibody or polyclonal rabbit anti-HA antibody (1:1000 dilution; Invitrogen) for 24 h at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to wells containing primary antibodies (1:5000 rabbit anti-c-Fos [SySy]; 1:500 chicken anti-GFP [Invitrogen]) and allowed to incubate on a shaker overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The cells were then washed 3x PBS and incubated with species-specific Alexa Fluor 488 conjugated goat anti-rabbit or Alexa Fluor 594 conjugated goat anti-mouse secondary antibodies (1:300; Invitrogen). The cells were then washed 3x PBS and mounted on a 1.5 mm coverslip with a Prolong™ Gold Antifade Mountant with DAPI.
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bioRxiv - Microbiology 2021Quote: ... secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 from Invitrogen and goat anti-mouse Alexa Fluor 647 from Invitrogen) were added and incubated for 30 min protected from light ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... Alexa Fluor 488 Goat anti-mouse antibody and Alexa Fluor 568 Goat anti-rabbit antibody were obtained from Life Technologies and used at 1:2000 for immunofluorescence.
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were then washed by rocking for 3x quick and 3x 10min in PBS-T and incubated with donkey anti-rabbit or anti-mouse secondary antibody (Invitrogen) for 1hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then PBS washed and incubated with the appropriate fluorophore-conjugated secondary antibodies in blocking solution (Donkey anti-Rabbit, Alexa Fluor 568, A10042, Invitrogen, Eugene, OR, US; Donkey anti-Mouse, AlexaFluor 488, A21202, Invitrogen, Eugene ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor conjugated secondary antibodies (donkey-anti-rabbit Alexa-488, ThermoFisher Scientific, C22841, donkey-anti-rabbit Alexa-555, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then washed 4× 2 h in PBT and incubated overnight in PBT supplemented with 1:500 goat anti-mouse or goat anti-rabbit secondary antibodies coupled either to Alexa Fluor 488 or 568 (Invitrogen – Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were incubated for 1 hour with Alexa Fluor 647 goat anti-mouse and Alexa Fluor 488 goat anti-rabbit (Invitrogen) or Alexa Fluor 546 goat anti-rabbit at 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then stained with secondary antibodies: Alexa Fluor 488 anti-mouse or Alexa Fluor 488 anti-rabbit (ThermoFisher Scientific) for 1.5 hours at room temperature and washed in the same manner ...
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bioRxiv - Developmental Biology 2022Quote: ... and secondary antibodies included anti-chick or anti-mouse conjugated either with AlexaFluor 488 (1:500 dilution; A11039 and A11001, respectively, Invitrogen) or with AlexaFluor 568 (1:500 dilution ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... Secondary antibodies included goat anti-rabbit and goat anti-mouse from Novus conjugated to DyLight 650 or Janelia Fluor 646 and goat anti-mouse from ThermoFisher Scientific conjugated to Alexa Flour 488 for immunofluorescence ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T helper cell subsets were identified using the antibodies listed above and the addition of anti-IL-5 (for Th2) and anti-IFNγ (for Th1) after permeabilization (Invitrogen). Gates were set according to Fluorescence Minus One (FMO ...
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bioRxiv - Developmental Biology 2022Quote: ... blocked for 20 min and incubated for 2h at room temperature with secondary antibodies Alexa Fluor 647-conjugated goat anti-mouse and Alexa Fluor 568-conjugated goat anti-rabbit (Invitrogen), used at 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody detection was performed with Alexa Flour 488 goat anti-mouse and Alexa Fluor 594 goat anti-rabbit (Invitrogen). Cells were counterstained with DAPI (1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then washed and incubated with Alexa Fluor labeled secondary antibodies (anti-mouse AF 555 and anti-rabbit AF568, Invitrogen) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... the tissues were stained with 1:2000 goat anti-mouse Alexa Fluor 594 and goat anti-rabbit Alexa Fluor 488 secondary antibodies (Invitrogen) at 25°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Microbiology 2022Quote: ... AlexaFluor 488 goat anti-rabbit IgG (1:500) and AlexaFluor 594 donkey anti-rat IgG (1:500) (Life Technologies, UK), as well as DAPI (1:3000 ...
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bioRxiv - Physiology 2022Quote: ... Alexa Flour 594-and Alexa Flour 633-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific, 1:500). Sections were mounted in DAPI-containing mounting medium (Vectashield H-1200 ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were then washed three times in PBST for 10 minutes each and incubated with secondary antibodies (goat anti-rabbit AlexaFluor488, ThermoFisher, cat. no. A11034; goat anti-chicken AlexaFluor488, ThermoFisher, cat ...
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bioRxiv - Microbiology 2019Quote: ... and subsequently stained with a primary murine anti-IE1 antibody and a secondary Alexa 647 Goat anti-mouse antibody (Thermofisher). Nuclei were additionally stained with Hoechst 33342 (Thermofisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... Coverslips were then washed three times in PBS before incubation with secondary antibodies (Alexa Fluor 555 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit; Invitrogen) at room temperature for 1 hour in a humidified chamber ...
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bioRxiv - Bioengineering 2019Quote: CD8+ and CD4+ T cells were purified from PBMC and activated by stimulation with anti-CD3/anti-CD28 beads (Thermofisher) in separate cultures ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 488 and Alexa Fluor 568 conjugated Goat Anti-Rabbit IgG (H+L) and Anti-Mouse IgG (H+L) (Invitrogen) and Alexa Fluor 405 conjugated Goat Anti-Rabbit IgG (H&L ...