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Citations for Thermo Fisher :
7001 - 7050 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The human pancreas adenocarcinoma cell line Bx-PC3 obtained from ATCC (ATCC CRL-1687) was grown in RPMI-1640 medium (ThermoFisher Scientific) supplemented with 10% fetal calf serum at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... human female adenocarcinoma of the mammary gland) were maintained in Dulbecco’s Modified Eagle Medium High Glucose (ThermoFisher Scientific, #11-965-118), 10% foetal bovine serum (Bovogen ...
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bioRxiv - Cell Biology 2021Quote: ... Transferrin, Selenite) premix (resulting in 10 µg/mL insulin, 5 µg/mL human transferrin and 30 nM sodium selenite) (Life Technologies). One droplet (10 µL ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Genes encoding full-length human TAK1 and Mfn2 were fused with a 5’ hexahistidine-tag by PCR and then cloned into pFastBac1 vector (Life Technologies). Once constructed ...
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bioRxiv - Molecular Biology 2022Quote: ... Whitehead Institute Center for Human Stem Cell Research, Cambridge, MA) (Lengner et al., 2010) were maintained on MEFs in hESC media (DMEM/F12 [Thermo Fisher Scientific] supplemented with 15% fetal bovine serum [FBS] [Hyclone] ...
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bioRxiv - Bioengineering 2022Quote: ... a human keratinocyte cell line featuring constitutive expression of human telomerase reverse transcriptase (hTERT) were cultured in FAD medium (3:1 DMEM : Ham’s F12 Medium (ThermoFisher®, 11320074), supplemented with 1% PS ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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bioRxiv - Microbiology 2022Quote: ... Fibrinogen aggregates were stimulated using 0.2mg/mL of purified fibrinogen from human plasma with an Alexa Fluor 488 conjugate (Invitrogen, Waltham, Massachusetts). Images were collected using an Olympus FluoView FV10i Confocal Laser Scanning Microscope under 60x magnification with an additional 2x zoom ...
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bioRxiv - Immunology 2022Quote: ... and human lung fibroblast (MRC-5 cells, ATCC® CCL-171™) were cultured in Minimum Essential Media (MEM, Life Technologies) supplemented with 4% FBS and 2 mM L-glutamine at 37°C in an atmosphere of 5% CO2.
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1-hTERT (female, human epithelial cells immortalized with hTERT, Cat. No. CRL-4000) from ATCC were grown in DMEM (Life Technologies) or DMEM/F12 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/4500) (all from Jackson Immunosearch Laboratories, Inc.) or goat anti-human IgG (γ-chain specific) HRP conjugate (1/50 000) (Invitrogen) were added and samples were incubated at 20-24°C for 60 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... MEF dPS-/- (presenilin 1 and 2 knock out) and the human neuroblastoma SH-SY5Y cell lines (ATCC) were grown in DMEM/F12 medium (Thermofisher Scientific) and were supplemented with 10% of FBS ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in a confluent monolayer of human foreskin fibroblasts (HFFs) maintained in Dulbecco’s Modified Eagle medium GlutaMAX™(DMEM+ GlutaMAX™, Gibco) supplemented with 10% foetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... IgG opsonized cells were washed and incubated with 3μg/ml of an APC-conjugated anti-human total IgG secondary Ab (Invitrogen, catalog A21445) at 4°C for 25mins ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC-O cells were cultured on mouse embryonic fibroblasts (MEFs) and proliferated using a medium for human ES cells [KNOCKOUT DMEM (Gibco,10829), 15% CTS KnockOut SR XenoFree Medium (Gibco,14150) ...
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bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 TM were resuspended in 1 mL RPMI media and either left unstimulated or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Microbiology 2022Quote: ... An inoculum of 2.5×104 CFUs of bacteria in 25 uL was mixed with 75 μL human serum from healthy volunteers (Cat# BP2657100; Fisher Scientific). The mixture was incubated at 37 °C and aliquots were taken at baseline and 1h to calculate viable CFUs ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... of human CD58 (UniProt residues 29-–204 + AviTAG + 6xHis) and human CD86 (UniProt residues 24–238 + AviTAG + 6xHis) were produced in Freestyle 293F suspension cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney HEK293T (293T) cells were passaged and maintained in Minimum Essential Medium (MEM + GlutaMAX, ThermoFisher Scientific) with 8.3% fetal bovine serum (FBS ...