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Citations for Thermo Fisher :
7251 - 7300 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: MRC5 fibroblasts (ATCC cat. no. CCL-171) and adult human mesenchymal cells were cultured in DMEM (cat. no. 11965092, Thermo Fisher) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and processed for quantitative PCR analysis of SMAD3 and SP1 binding to the human MCEMP1 promoter using Power SYBR Green PCR Master Mix (ThermoFisher Scientific). ChIP-qPCR results were calculated using the ΔΔCt method and are shown as percentage of input DNA for each ChIP experiment.
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was isolated from tissue lysates as well as from cultured human and murine aortic or DA SMCs with the PureLink RNA Mini Kit (ThermoFisher, 12183018). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Cell line derived from human neuroblastoma (SH-EP; CVCL_RR78) was verified by STR profiling and grown in RPMI-1640 (Thermo Fisher Scientific). Murine neuroblastoma cells (NHO2A ...
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bioRxiv - Immunology 2024Quote: ... tissue samples with small volumes (such as human samples) were then included in 1.5% agarose (Invitrogen, Carlsbad, CA, USA; #16500-500). Tissue clearing was then carried out with MetOH dehydration (as described above ...
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bioRxiv - Biochemistry 2024Quote: Human liver specimens were minced to extract genomic DNAs (gDNAs) using the PureLink™ Genomic DNA Mini Kit (Invitrogen™, K182001), and genomic DNA concentrations were measured using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Bioengineering 2024Quote: ... Human dermal fibroblasts (ZenBio, Cat. # DF-F) were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Cat. # SH30021FS) with 10% exosome-depleted FBS (Bio-Techne ...
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bioRxiv - Cell Biology 2024Quote: IMR-90 human fibroblast was purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... on semiconfluent monolayers of OP9 stromal cells expressing the human Jag2 Notch ligand (OP9-Jag2) (60) and cultured in p24-well plates with α-MEM medium (Gibco) supplemented with 20% FBS ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Microbiology 2024Quote: MRC-5 human embryonic lung fibroblasts were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco Thermo Fisher) supplemented with 10% FBS (Gibco Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Additionally, human embryonic kidney HEK293T/17 cells (ATCC, Manassas, VA, USA, Cat# CRL-11268) were grown in DMEM media (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The human neuroblastoma cell line (SH-SY5Y: ATCC, CRL-2266) was grown in low glucose DMEM medium (11885084, Thermo Fisher Scientific) with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Cancer Biology 2024Quote: RNA isolation and RT-qPCR for human tissue were performed as described previously (O’Donnell et al., 2023) using a hybrid TRIzol (Life Technologies 15596018) and RNeasy Plus Mini kit protocol after liquid nitrogen pulverization ...
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bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... and growth factors using the multiplexed-bead assay Non-Human Primate Cytokine & Chemokine & Growth Factor 37-plex ProcartaPlex (Invitrogen, Life Technologies), following manufacturers’ instructions ...
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2020Quote: ... cells were then washed 3 times with PBS and incubated with goat anti-rabbit Alexa Fluor 488 or goat anti-human Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific) for 1h at RT ...
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: The pCS2 Lrp6-APEX2-IRES-GFP-PAC plasmid was transfected into Human Embryonic Kidney 293T (HEK293T) cells using Lipofectamine2000 (Thermo Fisher Sci.). 48 hours after transfection puromycin was added to the culture medium ...
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bioRxiv - Immunology 2021Quote: ... and on the surface of H1568 EVs (with IFN-γ stimulation) were quantified using a PD-L1 Human ELISA kit (#BMS2212, ThermoFisher Scientific). For the cells ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA samples from affected individuals and unaffected family members were subjected to WES using Agilent SureSelect™ human exome capture arrays (Life Technologies) with next generation sequencing (NGS ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were immunostained according to standard protocols [21] using a primary rabbit anti-human antibody directed against zona occludens-1 (ZO-1; 1:100, Thermo-Fisher Scientific, UK) and a AF488-conjugated secondary goat anti-rabbit antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... 200,000 cells were transfected in 12 well plate with 25 pmol siRNA (siTFEB : ON-TARGETplus Human TFEB, L-009798-00-0005, Dharmacon™) using Lipofectamine RNAiMAX Transfection Reagent (1:200; Life Technologies). Cells were incubated 72 h at 37°C prior to further manipulation or drug treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were then incubated with Alexa 594 goat-anti rabbit secondary antibody at 1:750 and with Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT.
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bioRxiv - Neuroscience 2021Quote: ... cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific) or SP6 polymerase mMessage mMachine kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cultured in 10% human AB serum (produced in house) and 100 U/mL penicillin and 100 µg/mL streptomycin (Thermo Fisher Scientific) supplemented RPMI 1640 medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized primary human fibroblasts (gift from Paola Scaffidi) expressing human telomere reverse transcriptase (hTERT) (Scaffidi and Misteli, 2011) were grown in MEM (Thermofisher, 11095-080) supplemented with 15% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Media were collected after 24h in culture and cytokine/chemokine concentrations analyzed using the Inflammation 20-Plex Human ProcartaPlex™ panel (EPX200-12185-901, Thermo Fisher) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Immunology 2021Quote: ... or unseparated PBMCs (1 × 105 per well) were stimulated for 48-72 hours either with Dynabeads™ Human T Activator CD3/CD28 antibody coated beads (Thermo Fisher) at 2:1 or 4:1 bead:cells ratios or with plate-bound anti-CD3 and soluble anti-CD28 ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with Aß-S8C dimer was subjected to hybridization in duplicates on the GeneChip PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Cell Biology 2019Quote: ... and human ELYS-IDR-C2 (aa. 2034–2275) were artificially synthesized with the codon usage optimized for bacterial expression (Thermo Fisher Scientific), and sub-cloned into pET28-a (+) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated from the right middle lobe of mice and from pathologic samples from human lung tissue using Trizol (Invitrogen, Carlsbad, CA). Reverse transcription utilized the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... iPSCs were cultured on a feeder layer of irradiated mouse embryo fibroblasts using human embryonic stem cell media containing DMEM with F12 (DMEM/F12, 1:1 ratio, Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...