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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... IL-1α (1:50, Thermo Fisher PA5-89037), IL-1β (1:500 ...
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bioRxiv - Immunology 2023Quote: ... IL-1B (Thermo Fisher Scientific, (88-7261-77), IL-18 (Biotechne ...
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bioRxiv - Cell Biology 2023Quote: ... 20ng/ml IL-6 (RP8619, Invitrogen, United States) and 10ng/ml lipopolysaccharide (LPS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Neuroscience 2023Quote: ... horse serum (Thermo Fisher Scientific, Rockford, IL, USA), human NGF (hNGF ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 100 ng/mL IL-21 (Gibco) and 0.5 µg/mL R848 (Mabtech ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 U/mL of IL-2 (Gibco) were added every two days.
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bioRxiv - Neuroscience 2023Quote: ... or IL-17A ELISA kit (Invitrogen #88-7371) according to the manufacturers recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... cleaved IL-1β (1:100, PA5-105048, Invitrogen), or phospho-tau-T231 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... The IL-8 Canine ELISA Kit (#ECCXCL8, Invitrogen) and TNF-α Canine ELISA Kit (# ECTNF ...
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bioRxiv - Immunology 2023Quote: ... IL-1β (P420B, Thermo Fisher Scientific, 1:200),TNF-α (60291-1-Ig Proteintech ...
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bioRxiv - Bioengineering 2024Quote: ... and assayed with an IL-10 ELISA (Invitrogen). IL-10 and Fab-IL-10 generated in our laboratories served as the standards for their respective ELISAs ...
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bioRxiv - Immunology 2024Quote: ... mixed with Alum adjuvant (Thermo Scientific, Rockford, IL). Two weeks later ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Immunology 2020Quote: ... Capture antibodies were anti-IL-6 (14-7061-85, eBioscience), anti-TNF-α (14-7423-85, eBioscience) and anti-IL-1β (14-7012-81, Invitrogen). Detection antibodies conjugated with biotin were anti-mouse-IL-6 (554402 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2023Quote: ... IL-1β and IL-18 levels were assayed in supernatant by duplicate using commercial ELISA kits (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected using JetPEI® DNA transfection reagent (CHO dhFr- cells, Polyplus Transfection, Illkirch-Graffenstaden, France) or lipofectamin®2000 reagent (COS-7 and MDCK cells, Invitrogen, Carlsbad, US). Experiments were performed 24h (TIRF imaging ...
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bioRxiv - Immunology 2021Quote: ... the transfected cells were transferred to T-75 flasks at a 2 × 106 vc/mL density for a 1-day recovery in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) with 6mM GlutaMAX (Gibco; Waltham, MA, USA). After 1-day recovery ...
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bioRxiv - Immunology 2021Quote: Sigma Aldrich’s CHOZN cells were cultured in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) supplemented with 6 mM GlutaMAX (Gibco, Waltham, MA, USA), shaking at 37°C ...
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bioRxiv - Biochemistry 2022Quote: For confocal microscopy 25,000 adherent CHO-K1 cells were seeded on 8-well Nunc™ Lab-Tek™ (Thermo Scientific™, United States) and incubated overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The PACRG/pEGFP-N2 and DNALI1/Flag were transfected individually or together into CHO cells by using Lipofectamine™ 2000 transfection reagent (Invitrogen, Waltham, MA). The CHO cells were cultured with DMEM (with 10% fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: ... of Chinese hamster ovary (CHO) cell cultures using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well blocks (ThermoFisher Scientific). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Chinese hamster ovary cells (CHO) (RRID: CVCL_0214) were grown at pH 7.4 in filtered sterile F12 (Ham’s) nutrient medium (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 5% FBS and maintained in a humidified environment at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: CHO-K1 and the GAG-deficient variant CHO-745 cells (generous gift from Dr. Antonio Alcami, Spanish Research Council, Spain) were grown in DMEM/F12-Glutamax (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... The CHO-K1 cells were grown in low glucose (1.0 g/L) Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, Cat. no. 11,885-084) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: Total RNA extraction and isolation from normal and PSC liver cells or 3D-CHOs were performed using Trizol™ reagent and a PureLink RNA kit (Invitrogen, Waltham, MA). cDNAs were synthesized with a SuperScript™ First Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Chinese hamster ovary (CHO) cells transfected with CXCR3A were cultured in Ham’s F-12 growth medium (Gibco; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Flp-In CHO cells were chemically co-transfected with the linearized rDNA and circular pOG44 Flp-recombinase vector using Lipofectamine 3000 (Thermo Fisher Scientific, USA) following their protocol ...
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bioRxiv - Biochemistry 2023Quote: ... of paired heavy and light chain antibody sequences cloned into a single expression vector and expressed in Chinese hamster ovary (CHO) cells using the Gibco™ ExpiCHO™ Expression System and a protocol for deep 96-well plates (ThermoFisher Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... samples were resuspended in binding buffer containing a 1:100 dilution of mouse anti-human-IgG1-Fc conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL; A-10631) and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2023Quote: TNFα and IL-6 released in the microglia conditioned medium were quantified using mouse TNFα and IL-6 ELISA kits according to manufacturer’s instructions (Invitrogen, 88-7324 and 88-7064-22, respectively) and a SpectraMax i3x spectrophotometer (Molecular Devices) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience, cat#88731477, IL-2: Invitrogen eBioscience, cat#88702477, IL-17A: Invitrogen eBioscience, cat# 88737188). The supernatants were analyzed at various dilutions (1:2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted IL-10 using the IL-10 Rat ELISA Kit (Invitrogen, catalog # BMS629). The resulting values were normalized based on the cell viability ...
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bioRxiv - Immunology 2022Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22, murine IL-6: Invitrogen 88-7064-88) and a SpectraMax M2 plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng/mL IL-18 (Invitrogen, cat#rcyec-hil18) or 500 U/mL IFNg (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... swinholide A (cat. 501146229, Fisher Scientific, Hanover Park, IL), blebbistatin ...
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bioRxiv - Biophysics 2020Quote: ... An ECL detection system (Thermo Scientific, Pierce, IL, USA) was used to detect the protein signals ...
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bioRxiv - Cancer Biology 2020Quote: ... and visualized by chemiluminescence (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2021Quote: ... and 0.5% glutaraldehyde (cat no. BP25481, Fisher Scientific, IL) to improve the gel adhesion ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and subjected to IL-1β (Invitrogen) and TNF-α (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... determined by BCA protein assay (Thermo Scientific, Rockford, IL), were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with intracellular antibodies (Biolegend: IL-17A; ThermoFisher: IL-2 AF488 ...
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bioRxiv - Microbiology 2019Quote: ... from Biolegend and IL-4-Alexa Fluor 488 (11B11) from Invitrogen. Live cells were identified using fixable live dead aqua (Life Technologies) ...
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bioRxiv - Biophysics 2019Quote: ... An ECL detection system (Thermo Scientific, Pierce, IL, USA) was used to detect the protein signals ...
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bioRxiv - Biochemistry 2019Quote: ... OPA reagent was purchased from Thermo Scientific (Rockford, IL). Materials used for cell culture were from Gibco by Life Technologies (Grand Island ...