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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... These confluent CHO cells were transfected with 700 ng pUNO1-Mincle or pUNO1-DC-SIGN using TurboFect Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The AF-03 and AF03-NL plasmid were transfected into ExpiCHO-S cells using the ExpiFectamine™ CHO Transfection Kit (Gibco, A29129) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... microscale transfection was performed (∼1 ml per antibody) with CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ T cells were co-cultured with CHO cells for 16 hours in the presence of T cell activation using anti-CD3/CD28 Dynabeads™ (ThermoFisher Scientific) at an estimated 1:1 bead-to-cell ratio ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: IL-1β levels were determined from prefrontal cortex extracts by using the IL-1β ELISA kit (ThermoFisher Scientific, #88-7013) following the manufacturer’s protocols.
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Immunology 2022Quote: ... and stimulated for 24 hours with IL-17A (200ng/mL, cat: 200-59-100ug, Shenandoah) and anti-IL-17RC (5ug/mL, cat: PA5-47286, Invitrogen). Goat IgG (5ug/mL ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at room temperature for 1 h with secondary antibodies Alexa Fluor 568 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (1:500; Invitrogen Life Technologies, Rockford, IL, USA), counterstained with Hoechst 33258 (1:1,500 ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and incubated with mouse anti-HA mAb clone 12CA5 conjugated and crosslinked to protein A/G magnetic agarose beads (Pierce; Rockford, IL. Thermo Fisher Scientific. Waltham, MA, USA). After rotation at 4°C for 1 h ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL) was then used to evaluate RNA purity ...
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (eBio17B7, Thermo Fisher Scientific, Waltham, MA), and IFNγ (XMG1.2 ...
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bioRxiv - Neuroscience 2019Quote: ... and protease inhibitor cocktail (Thermo Scientific, Rockford, IL)] ...
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bioRxiv - Immunology 2021Quote: ... and IL-33 (Invitrogen; sensitivity 25 pg/mL) ELISA kits according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-8 Monocyte Recombinant Human Protein (ThermoFisher, PHC0884), and Recombinant Human CXCL1/GROα Protein (R&D Systems ...
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bioRxiv - Immunology 2020Quote: Detection of human IL-1β and TNFα (Invitrogen) in hMDM culture supernatants were performed by sandwich ELISA ...
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bioRxiv - Immunology 2020Quote: ... IL-1α (Thermo Fisher Scientific, clone ALF-161), or IL-1β (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or IL-1β (Thermo Fisher Scientific, clone NJTEN3) in perm/wash buffer for 30 min at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... ELISA assays for IL-6 (Invitrogen, catalog # KHC0061) and IL-8 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and IL-17 (Invitrogen Cat. 88-7371-88) was quantified by a sandwich ELISA according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... or IL-17 at (2.5 ng/ml; Invitrogen), following the protocol previously published for RASFs113 ...
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bioRxiv - Immunology 2020Quote: ... 10 ng/ml IL-2 (Gibco, Gaithersburg, MD), and 25 ng/ml IL-10 (STEMCELL Technologies ...
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bioRxiv - Physiology 2021Quote: ... and trypsin (Thermo Fisher Scientific, Rockford, IL, USA), overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... respectively (Thermo Fisher Scientific, Meridian Road Rockford, IL) and analyzed as detailed in Calle et al ...
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bioRxiv - Microbiology 2020Quote: ... and anti-IL-17A antibody (Thermo Fisher Scientific) were used ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 was purchased from ThermoFisher Scientific.
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ORAI1 establishes resistance to SARS-CoV-2 infection by regulating tonic type I interferon signalingbioRxiv - Microbiology 2021Quote: ... or IL-6 (ThermoFisher Scientific, # 88-7066-22) using manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml IL-4 (Life Technologies), and evaluated on day 6 of culture ...
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bioRxiv - Immunology 2022Quote: ... PerCP-eFluor710-IL-13 (eBio13A, Thermo Fisher Scientific), PE-IL-5 (TRFK5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng IL-2 (ThermoFisher Scientific, Cat#PHC0027).
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bioRxiv - Cell Biology 2022Quote: ... and 1 ng/ml IL-6 (Invitrogen, PHC3011)) (Wimmer ...
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bioRxiv - Microbiology 2022Quote: ... modified Lowry method (Thermo Fisher Scientific, IL, USA), and Folch method (Folch et al. ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-17F PerCP-ef710 (SHLR17, Thermo Fisher), anti-IFN-γ PE-Cy7 (XMG1.2 ...
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bioRxiv - Immunology 2022Quote: ... anti-IL-4 (Thermo Fisher; 14-7041-85), biotin labeled anti-IL-4 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10ng/ml recombinant human IL-3 (Gibco). Cultured cells were infected with lentivirus encoding GFP/Scrambled control shRNA overnight and fresh media was added the following day ...
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bioRxiv - Biochemistry 2023Quote: ... in pyridine (CAS#25104, Thermo scientific, Rockford, IL) and stirred at 90 ° C for 1.5 hours ...
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bioRxiv - Biochemistry 2023Quote: ... anti-MyoD mAb (ThermoFisher, IL, US, MA1-41017); anti-collagen type I alpha1 chain (COL1A1 ...