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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and differentiated towards hemogenic endothelium with growth factors 50 ng/mL BMP4 (Invitrogen), 50 ng/mL VEGF (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng/ml Epidermal Growth Factor (EGF, Fisher Scientific PeproTechR; AF-100-15), 0.5 μg/ml hydrocortisone (Calbiochem ...
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bioRxiv - Systems Biology 2024Quote: ... on growth factor reduced geltrex basement membrane extract (Life Technologies, 10 μg/cm2) coated dishes at 37°C in a humidified atmosphere of 5% CO2 in air.
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bioRxiv - Neuroscience 2024Quote: ... and 5 ng/mL human fibroblast growth factor (#13256-029, Thermo Fisher Scientific)] (proliferative conditions) ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilised using Foxp3 Transcription Factor Staining Buffer Set (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... in conjunction with a FOXP3 Transcription Factor Staining Buffer Kit (Thermo Fisher Scientific). Intracellular cytokines were exposed using a Cytofix/Cytoperm Plus Fixation/Permeabilization Solution Kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... supernatants were removed for soluble factor analysis and a LIVE/DEAD® (Invitrogen) stain was applied with subsequent imaging and fluorescence quantification followed by a CellTiter-Blue® (Promega ...
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bioRxiv - Immunology 2024Quote: ... The FOXP3/Transcription factor staining buffer kit (00-5523-00; Thermo Fisher Scientific) was used for fixation ...
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bioRxiv - Bioengineering 2024Quote: ... Using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00), cells were then permeabilized in 1 mL fixation/permeabilization solution and incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μM platelet-derived growth factor-AB (PDGF-AB, ThermoFisher, 100-00AB-10UG), 10 ng/ml tumor necrosis factor alpha (TNF-α ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse epidermal growth factor (mEGF, 20 ng/mL; Thermo Fisher, Cat. #PMG8044, Norway), and 1x B27 supplement (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... together with niche factors: 50 ng/mL for EGF (Thermo Fisher Scientific, USA), 500 nM for TGF-β receptor type I inhibitor A83-01 (MCE ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with recombinant mouse stem cell factor (SCF, 100 ng/mL, Thermo Fisher), recombinant mouse Interleukin-6 (IL-6 ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed again 3 times for 10 minutes with 1x TBST and proteins were visualized using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). Signals were digitally captured on a Fusion Solo S (Witec ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of protein (in vitro samples: 3 μg; in vivo samples: 80 μg) were separated by SDS-PAGE (Thermo Fisher) and transferred to a 0.45 μm nitrocellulose membrane (Amersham) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The dried peptides were resuspended in 0.1% FA and 3% ACN in water and protein quantity measured at A280 via NanoDrop (Thermo Fisher Scientific). Concentrations were normalized across all fractions and iRT (Biognosys ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 3-5 µg of protein was subjected to nanoflow liquid chromatography on an EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) on-line coupled to an Q Exactive HF quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Biochemistry 2022Quote: ... the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific) to remove the 6×His tag ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: The lysate and purified proteins were incubated with 3 µg of biotinylated RNA probe coupled to 20 µl Dynabeads MyOne Streptavidin C1 (Thermo Fisher) in 600 µl lysis buffer at 4°C for 1 hour rotating head-over-tail ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: Flash frozen samples (n=3 per genotype) were prepared for Western blot using the Mem-PER Plus protein extraction kit (89842, Thermo Scientific) to isolate membrane and cytoplasmic protein fractions from mice aged P29-32 ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a commercial native- PAGE gel (3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well, Invitrogen, Cat. BN1001BOX) at 100 V tension using as running buffer 50 mM BisTris ...
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Genetics 2020Quote: ... Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific). 1 mM ssODNs (PIK3C2B ...
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bioRxiv - Immunology 2020Quote: Aag2 cells were seeded at 1 × 106 cells/well in 12-well plates and transfected at the time of seeding with 3 μg protein expression plasmid using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting membrane pellet was resuspended in buffer M10 at 3 mL/g (of starting mitochondria) and the protein concentration was determined using a BCA assay (Pierce Thermo Fisher). The resuspended membranes were stored at 10 mg/mL of total protein at -80°C in a final glycerol concentration of 30% (v/v ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions with the greatest quantity and purity of UBC9 protein combined and concentrated down to 5 ml using 3-kDa MWCO centrifugal concentrator (Thermo Scientific) at 4,000xg/4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... was used to quantify protein concentration and protein samples were diluted to 3 μg/μL in Laemmli SDS sample buffer (catalog number J61337.AC, Thermo Fisher) and boiled at 100°C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: Fluorescent labelling of Tau and 14-3-3ζ protein for microscopy assays was done using amine-reactive DyLight405 or DyLight488-NHS ester (Thermo Scientific) following the manufacturer instructions and the established protocol 34.
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were separated by magnet and washed with IP lysis buffer 3 times before proteins were eluted by heating in 2xNuPAGE LDS sample buffer (Invitrogen, NP0008) and 2x sample reducing buffer (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...