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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... and 25 µg·mL-1 fibroblast growth factor (fGFb; #PHG0026, Thermo Fisher Scientific). The cells were plated in flasks pre-coated with an extracellular matrix (ECM ...
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bioRxiv - Cell Biology 2024Quote: ... on plates coated with Geltrex reduced growth factor basement membrane (Thermofisher A1413302). For routine passaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µL fibroblast growth factors (100 µg/mL FGF) obtained from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2024Quote: ... Epidermal growth-factor (EGF) was purchased from Thermo Fisher (Cat. No.: PHG0314) and diluted in sterile PBS to a final concentration of 100 µM (stock solution).
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bioRxiv - Molecular Biology 2024Quote: ... 50 μg/ml recombinant mouse epidermal growth factor (EGF; Thermo Fisher Scientific), 2 μl/ml Primocin® (InvivoGen ...
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bioRxiv - Immunology 2024Quote: ... the eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific, Cat# 00552300) was used as follows ...
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bioRxiv - Immunology 2024Quote: ... fixed and permeabilized using FoxP3/Transcription Factor Staining Buffer Kit (Thermo Fisher) according to the manufacturer’s protocol and stained with anti-FoxP3-PE (clone PCH101 ...
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bioRxiv - Immunology 2024Quote: ... The Foxp3/transcription factor staining buffer set (eBioscience/Life Technologies – Thermo Fisher) was used for the intracellular staining of FoxP3.
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bioRxiv - Immunology 2024Quote: ... The Foxp3/transcription factor staining buffer set (eBioscience/Life Technologies – Thermo Fisher) was used for the intracellular staining of FoxP3.
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bioRxiv - Immunology 2024Quote: ... cells were fixed using the Foxp3/transcription factor staining kit (ThermoFisher Scientific) followed by fixation with 90% methanol for 15 min ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed using the Foxp3/transcription factor staining kit (ThermoFisher Scientific) according to the manufacturer’s protocol and stained with Ki-67 (SolA15 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 4 ng/mL of epidermal growth factor (Thermo Fisher Scientific Inc.) for 17 h at 37 °C under 5% CO2 in the air (Morohaku et al. ...
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bioRxiv - Immunology 2024Quote: ... fixed and permeabilized with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen), according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 5 ng/mL of human epidermal growth factor (hEGF, ThermoFisher), 20 ng/mL of nerve growth factor (NGF ...
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bioRxiv - Bioengineering 2024Quote: ... and 25 ng/ml nerve growth factor (NGF, 2.5S, Gibco, 13257-019)) ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5 ng/mL glial cell line-derived neurotrophic factor (GDNF; Gibco #PHC7045), 2.5 ng/mL brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL of nerve growth factor (cat no. 13257-019; Thermo Fisher), and 10 mg of ascorbic acid (cat no ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized with the FoxP3/Transcription factor staining buffer set (Thermo Fisher Sci.), and stained with conjugated antibodies targeting IL-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Molecular Biology 2020Quote: ... The insoluble material was removed by centrifugation and the protein content in the supernatant was measured using a Qubit 3 fluorometer (Invitrogen) and equal amounts of proteins were separated in SDS-10 % polyacrylamide gels and blotted onto a nitrocellulose membrane (Amersham) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then homogenized through a 26-gauge syringe 3 times and protein content measured by BCA (ThermoFisher; Waltham, MA). Samples were then diluted in 4X Sample Buffer (2% SDS ...
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bioRxiv - Developmental Biology 2022Quote: PLP F2-3 (aa 584 to 1811) protein was isolated from SF9 insect cells using Bac-to-Bac baculovirus expression system (Thermofisher). Coding sequence of PLP F2 -3 was cloned into pFastBac donor plasmid containing a Flag tag at the C-terminus of the insert site of PLP (Gift from Rong Liu ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Chromatin-antibody complexes were captured for at least 3 hr using a mix of Protein A and G Dynabeads (Invitrogen). The captured immunoprecipitated complex were washed 10 min each with sonication buffer (50 mM HEPES ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Biochemistry 2020Quote: ... The beads were washed 3 times then subjected for protein elution with 50 units of AcTEV protease (Invitrogen, 12575-015) in PBS buffer by 8 hr incubation in 4 °C on a rotator.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2020Quote: A total of 50 μg of protein for each of the samples was loaded into a pre-cast NativePAGE 3-12% Bis-Tris gel (Invitrogen). For the run ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 100 ng of yellow fluorescent protein (YFP) cDNAs in each well using 1:3 DNA: Lipofectamine 2000 (11668019, Invitrogen) ratio ...
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bioRxiv - Microbiology 2021Quote: ... Midguts were sonicated for 3 cycles of 15 seconds yielding approximately 100μg of protein from each pool as measured by BCA assay (Thermo Scientific). Samples were reduced with 5mM DTT for 30 mins at 50°C then alkylated with 15mM IAA for 30 mins at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or ribosomal protein large P0 (RPLP0) endogenous transcripts (Thermo Fisher Scientific; 4310884E; 4310879E) and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatants containing both the cytosolic and glycosomal proteins were analysed by BN-PAGE on a precast (3–12%) Bis-Tris polyacrylamide gel (Invitrogen) according to standard methods.
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bioRxiv - Genomics 2021Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were separated on a NativePAGE Novex 3%–12% Bis-Tris protein gel system (Thermo Fisher Scientific, catalog BN1003BOX) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The RNA-protein complexes were then subjected to 3’ adapter ligation and reverse transcription with SuperScript III Reverse Transcriptase (Invitrogen). The cDNA was purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... 5×106 cell equivalents were loaded into wells on a 3-12% Bis-Tris gradient gel with unstained NativePage protein standards in the first well (ThermoFisher) and run according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... An equivalent to 100 mg of mitochondrial proteins were loaded and separated on 3–12% (w/v) polyacrylamide NativePAGE Bis/Tris gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...