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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 6 CHLORO 3 METHYLPYRIDO 3 4 D PYRIMIDIN 4 3H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... 3 hours later cultures were washed and fixed with 4% paraformaldehyde (Electron Microscopy Solutions) in phosphate-buffered saline (PBS; Thermo Fisher) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Molecular Biology 2023Quote: ... and blotted on two sides for 3 sec at 4 °C and 100% humidity before vitrified in liquid ethane using a Vitrobot Mark IV (Thermo Fisher). The grids were subsequently transferred to a Titan Krios (G3i ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... One milligram of protein was incubated with 4 µg of antibody (IgG (Rb, Invitrogen) or HA (Rb ...
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bioRxiv - Genetics 2019Quote: ... The Tmem98 open reading frame without the initiating ATG was amplified by PCR using primers 5’-CACCGAGACTGTGGTGATCGTCG-3’ and 5’-AATGGCCGACTGTTCCTGCAG-3’ and cloned into pENTR™/D-TOPO™ (Thermo Fisher Scientific) and subsequently into pcDNA™6.2/N-EmGFP-DEST (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... The Tmem98 open reading frame with the initiating ATG was amplified by PCR using the primers 5’-CACCATGGAGACTGTGGTGATCGTCG-3’ and 5’-AATGGCCGACTGTTCCTGCAG-3’ and cloned into pENTR™/D-TOPO™ (Thermo Fisher Scientific) and subsequently into pcDNA™-DEST40 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cancer Biology 2019Quote: ... Spheroids pictures were taken after 3 and 6 days using EVOS FL microscope (Thermofisher) and the images were analyzed with ImageJ software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Microbiology 2023Quote: ... Substrate 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS; Thermo Fisher Scientific) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Brains were washed again as before and then incubated for 3h at room temperature followed by 24h at 4 °C with Alexa Fluor Plus 488 Goat anti-mouse (Life Technologies # A32723) and Alexa Fluor Plus 555 goat anti-rabbit (Life Technologies # A32732 ...
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bioRxiv - Cell Biology 2019Quote: ... and then manually segmented into 3-D models in Amira (FEI, Thermo Fisher Scientific). Some thresholding was used to expedite the process ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were detected by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA).
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bioRxiv - Molecular Biology 2020Quote: ... and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific) at 1:2,000 dilution at RT for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were counterstained in 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D1306) for 10 minutes at room-temperature and mounted in ProLong Gold Antifade (Thermo fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were labelled using 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Invitrogen) and coverslipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (1:10,000, Invitrogen) for 3 min and eventually coverslips were mounted with Dako mounting kit (Fluka) ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Life Technologies) at a dilution of 1:5000 in 1 X PBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counterstained using 4’ ,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei ...
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bioRxiv - Microbiology 2019Quote: ... Nuclei were then stained with DAPI (4’,6- diamidino-2-phenylindole, dihydrochloride, ThermoFisher, 62247 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei and finally washed with PBS.
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bioRxiv - Cancer Biology 2021Quote: ... The nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probes) at 1 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10 000, Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were staining with DAPI (4′,6-diamidino-2-phnylindole) from Molecular Probes. Confocal images were acquired using a Leica Sp8 confocal microscope and processed using Imaris image analysis software (version 9.3.1).
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bioRxiv - Cancer Biology 2022Quote: ... sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH; 22589, Thermo Fisher Scientific), 3-(Acryloyloxy)propyltrimethoxysilane (L16400 ...
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bioRxiv - Immunology 2022Quote: ... Nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI) dihydrochloride (Life Technologies), and lung sections were mounted on glass microscopy slides using fluorescence mounting medium (Dako) ...
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bioRxiv - Cancer Biology 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI, 0.1 μg/mL; #D1306, Invitrogen) and mounted with Prolong Gold antifade medium (#P10144 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to visualize the nuclei ...