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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were separated by a 140 min linear gradient from 5-30% ACN containing 0.05% acetic acid on an UltiMate 3000 UHPLC Nano (Thermo Fisher Scientific) coupled to Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... The dried peptides were taken up in 0.1% (v/v) formic acid and then loaded onto a PepMap100 C18 LC pre-column (75 μm I.D., 2 cm, Thermo Scientific) and eluted on line onto an analytical NanoViper PepMap 100 C18 LC column (75 μm I.D. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
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bioRxiv - Immunology 2022Quote: ... ileal tissue was collected and stored in stabilization buffer (2 mM ethylenediaminetetraacetic acid [EDTA; Invitrogen AM9260G], 2mM L-glutamine [Gibco 25-030] ...
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bioRxiv - Bioengineering 2020Quote: The quantification of BMP-2 initially loaded in the polyelectrolyte film (deposited at the bottom of a 96-well microplate) was done using a micro bicinchoninic acid assay (microBCA) test for low BMP-2 concentration (BMP20) while Nanodrop (Thermofisher) was used for higher concentrations ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at 2×106 cells/ml in T cell media (R10 supplemented with 1X non-essential amino acids (Gibco) + 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were re-suspended in 2 % formic acid and analysed using an UltiMate™ 3000 RSLCnano System (ThermoFisher Scientific) coupled on-line to an Orbitrap Exploris 480 (ThermoFischer Scientific) ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: Multiplexed peptide fractions from each time point were resuspended in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) to be analyzed on the Exploris 480 mass spectrometer equipped with FAIMS (ThermoFisher Scientific). The mass spectrometer was interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid chromatography used a 2-90% MeCN linear gradient in water with 0.1% formic acid on a C18 BioBasic analytical HPLC column (Thermo Scientific) installed on an Agilent 1290 HPLC system ...
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bioRxiv - Biochemistry 2021Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations of DTNB were added to protein and change in absorbance was measured in either an SX-20 Stopped Flow instrument (Applied Photophysics ...
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bioRxiv - Immunology 2020Quote: ... the desalted samples were first resuspended into 20 μl 0.1% formic acid in water and then loaded onto a trap column (2 cm × 300 μm, PepMap C18, Thermo Scientific) and an analytical column (19 cm × 75 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
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bioRxiv - Molecular Biology 2022Quote: The reconstituted peptides in solvent A (2% acetonitrile, 0.1% formic acid) were separated on a 50 cm long EASY-spray column (ThermoFisher Scientific) connected to an Ultimate-3000 nano-LC system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 µm ID × 15 cm, 2 µm; Thermo Scientific) mounted on a DPV ion source (New Objective ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were then dissolved in 2% ACN and 0.1% formic acid and analyzed on a Thermo Scientific Q Exactive Mass Spectrometer (ThermoFisher Scientific). The MS was operated in data-dependent mode ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Physiology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (Thermo Fisher Scientific) and a 35 cm long ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM × 250 mm) in Solvent A (98% water, 2%acetonitrile, 0.1% formic acid) via a Nanospray Flex Ion-Source (Thermo Scientific) at a voltage of 1.9 kV with a flow rate of 0.7 µL/min in Solvent A (98% water/2%acetonitrile/0.1% formic acid) ...
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Physiology 2024Quote: ... hearts were loaded with 1 mg/ml Rhod-2 AM in 0.5 mL of DMSO containing 10% pluronic acid (Molecular Probes) followed by 50 µL 1 mg/ml RH237 in DMSO (Molecular Probes) ...
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bioRxiv - Microbiology 2024Quote: ... 750 mM aminocaproic acid, 2 mg/ml lysozyme, 1 mM EDTA, 1.43 mM BME, 1x EDTA-free Pierce Protease Inhibitor [Thermo Scientific]) and incubated 20 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... from day 1-2 Sprague-Dawley rats and maintained in DMEM (L-glutamine, Sodium Pyruvate, Non-essential amino acids; Gibco) supplemented with 2% FBS (Gibco ...
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bioRxiv - Biophysics 2024Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations (100 -1000 μM ...
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bioRxiv - Bioengineering 2024Quote: ... with the pellet re-suspended in 0.1 M (2-(N-morpholino)ethanesulfonic acid) (MES) buffer at pH 5.5 (Fisher Scientific 15442958). This process was repeated four times ...
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bioRxiv - Cancer Biology 2024Quote: ... WiT49 + sgNTC-2 cells were transfected with 10 µg/ml polyinosinic-polycytidylic acid (Poly I:C) with Lipofectamine 3000 (Invitrogen L3000015). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of amino acids (1× MEM Non-Essential Amino Acids and 1× MEM Essential Amino Acids, both manufactured by Gibco™) was added with the glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of 5 mM aa-dUTP/dNTP mix (5 mM each dATP, dGTP and dCTP, 3 mM dTTP and 2 mM 5-(3-aminoallyl)-dUTP (Thermo Fisher Scientific, AM8439)) and 1.25 µL of 40 µM oligo 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: ... x 5 mm 5 μm 100 Å and an Acclaim PepMap RSLC 75 μm x 50 cm 2 μm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from BoDV-2-infected Vero cells was ligated with either 5’ adaptor oligoRNA (5’-CGACUGGAGCACGAGGACACUGACAUGGACUGAAGGAGUAGAAA (Thermo Fisher Scientific, USA; #L150201)) or 3’ adaptor oligoRNA (5’-GAAGAGAAGGUGGAAAUGGCGUUUUGG ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Hydrochloric acid and acetic acid were provided by Fisher Scientific (Loughborough, United Kingdom). 17α-methyltestosterone was delivered by Steraloids (Newport ...
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bioRxiv - Systems Biology 2024Quote: ... formic acid (FA) and acetic acid were purchased from Fisher Scientific (Pittsburgh, PA). Acrylamide was purchased from Acros Organics (NJ ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovaries from ∼2-5 flies were dissected in Schneider’s Drosophila media (ThermoFisher SKU #21720-001) that was also used for ex vivo culturing ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and loaded with 5 μM Rhod-2 AM (R1244, Invitrogen) in HBSS without calcium/magnesium for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were detached after 2 or 5 days of co-culture with StemPro Accutase (Thermofisher), experimental duplicates were performed for each timepoint and each condition ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Molecular Biology 2022Quote: ... 5 x 107 ESCs were fixed with 2 mM DSG (disuccinimidyl glutarate, Thermo Fisher Scientific) for 50 min at 25⁰C and then 1% formaldehyde (methanol-free ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...