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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 ml of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Biochemistry 2022Quote: ... cells were pulsed with 20 μM EdU (5-ethynyl-2′-deoxyuridine, Thermo Fisher) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... cells at 2×107 /mL with CTV at 5 μg/mL (Thermo Fisher) and incubating for 15 min at 37 °C followed by blocking in FBS and washing with complete medium according to the aforementioned protocol ...
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bioRxiv - Neuroscience 2022Quote: ... mice were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in 1x PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were intraperitoneally injected with 5-ethynyl-2’-deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μL of 200mg/ml 5-ethynyl-2’-deoxyuridine (EdU; E10187, ThermoFisher Scientific) was administered daily for 2 weeks by intra perennial injections into 4 week ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were pulsed with 1 mM 5-Ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 3 hr ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Proteins were reduced with 5 mM tris (2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90 °C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were treated with 10 μM 5-ethynyl-2’-deoxyuridine EdU (#E10187, Invitrogen) 24 hours prior to harvest ...
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bioRxiv - Cell Biology 2023Quote: Whole 5-day-old seedlings were incubated with 2 µM FM4-64 (Invitrogen) solution in half-strength MS liquid medium without sucrose at room temperature for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 mL of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Cancer Biology 2023Quote: ... or 5- (and -6)-chloromethyl- 2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA, ThermoFisher, C6827), or hydroxyphenyl fluorescein (HPF ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were treated with 10 µM 5-ethynyl 2’-deoxyuridine (EdU) (Invitrogen) for 15 min to visualize replicated DNA ...
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bioRxiv - Microbiology 2023Quote: Mice were injected with 100 μg of 5-ethynyl-2’deoxyuridine (EdU, Invitrogen) 2 hours prior to splenocyte harvest ...
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (Thermo Fisher) with 0.01% pluronic F-127 at 37°C for 45 min ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were incubated with 5 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Scientific) in 50 mM ammonium bicarbonate for 30 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fisher Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated in medium containing 20 mM 5-ethynyl-2’-deoxyuridine (Invitrogen) and 1 mM 5-fluoro-2’-deoxyuridine (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... we use the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific A10044). Procyclic of Cas9 and LmexNAT10-/+ cell lines were collected at logarithmic growth phase and incubated with 100 µM 5-ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Microbiology 2024Quote: ... treated with 5 µl of TURBO DNase (2 U/µl, Thermo Fisher Scientific) on ice for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 488 anti-mouse/human ɑ-tubulin (B-5-1-2; Invitrogen). Collagen gels were rinsed with PBS prior to imaging by confocal microscopy.
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bioRxiv - Biochemistry 2024Quote: ... and 5 mM tris(2-carboxymethyl)phosphine (Bond-BreakerTM TCEP, Thermo Fisher Scientific). In-solution digestion was performed overnight in a ThermoMixer at 2000 rpm at 37 °C with a protein-to enzyme ratio of 1 to 100 for both LysC (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR on the mouse Zdhhc17 gene using primers spanning exons 1 and 2 (5’-ACCCGGAGGAAATCAAACCACAGA-3’ and 5’-TACATCGTAACCCGCTTCCACCAA-3’) and Sso/Advanced Universal SYBR green supermix (Fisher Scientific) was performed on CFX96 Real Time System (C1000 Touch Thermal Cycler ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079 ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... or amino acids (MEM amino acids solution; Gibco, Waltham, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... Except for lauric acid (Henkel) and 1,18-Octadecanedicarboxylic acid (Thermofisher Scientific), lipids were from Merck ...
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bioRxiv - Cell Biology 2020Quote: ... Gel pieces were incubated in Elution buffer (1% formic acid, 2% acetonitrile in LC-MS grade water (Thermo Scientific)) and dried in a SpeedVac ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... we prepared a 2-fold dilution series of a 1x “stimulation” solution containing 1x essential amino acids (Invitrogen, 11130051), 1x non-essential amino acids (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... were quantified using an avidin and 4’-hydroxyazobenzene-2-carbocylic acid assay according to the manufacturer’s instructions (Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Cells were prepared for analysis by staining with 2 x SYBR Green I nucleic acid gel stain (Life Technologies) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... diluted 2 times with 0.1 % formic acid and desalted on strong cation exchange micro-tips (StageTips, Thermo Fisher scientific) as described72 ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 ul NET-2 and extracted with an equal volume of acid-phenol:chloroform (Invitrogen AM9722). After centrifugation at 16,000 x g for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 22 cm long ...
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bioRxiv - Cell Biology 2023Quote: ... by recording the increase in the absorbance at 405 nm after the reaction of reduced glutathione with 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) for 2.5 min at 15-s intervals using a Varioskan Flash (Thermo Fisher). GSSG was quantified after derivatization of GSH with 2-vinylpyridine ...
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bioRxiv - Bioengineering 2023Quote: ... 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB, aka Ellman’s Reagent) and DL-dithiothreitol (DTT) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2024Quote: ... fractions were resuspended in 2% acetonitrile and 0.1% formic acid and separated on an Easy nLC 1200 (ThermoFisher Scientific) and a 35 cm long ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Neuroscience 2024Quote: ... dried peptide samples were reconstituted with 2% ACN-0.1% FA (Acetonitrile-Formic acid) and quantified by NanoDropTM spectrophometer (ThermoFisher) before LC-MS/MS analysis using an EASY-1000 nanoLC system coupled to an Exploris 480 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and under a 12-hour day/night photoperiod schedule (Percival Scientific, IA, USA) with 5% dextrose (w/v) and 0.05% para-Aminobenzoic acid (w/v) (Thermo Fisher). Mosquito oocysts were checked at 10-days post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Genetics 2022Quote: ... as well as fibroblasts and SH-SY5Y cells treated for 5 hours with 100 µM oleic acid (OA) following over-night starvation in Opti-MEM (ThermoFisher). OA is a potent inducer of lipid droplet formation (55) ...