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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
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bioRxiv - Neuroscience 2024Quote: ... Control 1 (C1) and 2 (C2) were derived by StemBANCC from an unaffected volunteer and control 3 (C3) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
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bioRxiv - Cancer Biology 2022Quote: ... cell density was adjusted to 106 cells/mL (and was never allowed to go over 3×106 cells/mL) and puromycin selection (2 μg/mL, Gibco, A1113803) was started ...
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bioRxiv - Biochemistry 2022Quote: ... Between 2 to 3 million cells were used per electroporation and were resuspended in 90 μl of buffer R (Thermo Fisher). Proteins were spun down at 16700 g for 10 minutes and then diluted with buffer R in a 1:1 ratio ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher). Cryo-EM data were collected with a Titan Krios microscope (FEI ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 6 μL/min with 2% ACN ...
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bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
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bioRxiv - Genetics 2024Quote: 100,000 HEK293 cells in a 6-well plate were transfected with Antisense oligonucleotide harbouring 2’-O Me phosphorothioate modifications at each nucleotide position (Integrated technologies) using 3 ul of Lipofectamine 2000 (11668027, ThermoFisher Scientific) in one ml OPTIMEM I Reduced Serum Medium with no phenol red (11058021 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in cell culture dishes (Falcon) and passaged every 2-3 days via trypsinization with 0.0S% Trypsin-EDTA (2S300-0S4, Thermo Fisher Scientific) at 80-90% confluency ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 g of flash-frozen human prefrontal cortical post-mortem tissue was sliced with a razor blade into 2–3 mm3 sections in Hibernate-E media (Thermo Fisher) on ice ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was dialyzed against deionized water at 4 °C for 2 days using Slide-A-LyzerTM Dialysis Cassette (10K MWCO, 3 mL, Thermo Scientific) and stored at −70 °C.
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 9496 Movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... which was equipped with a 75 μm x 2 cm trap column packed with C18 3 μm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC ...
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bioRxiv - Biochemistry 2024Quote: ... onto a nanotrap column (75 μm i.d. x 2 cm precolumn, packed with Acclaim PepMap100 C18, 3 μm, 100 Å; Thermo Fisher) using an EASY-nLC 1200 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were first loaded on the trap column (Acclaim PepMap 100 C18, 75 µm ID x 2 cm, 3 µm particle size, Thermo Scientific), washed with 0.1 % formic acid in water for 7 min at 5 µL/min and subsequently transferred to the separation column (IonOpticks Aurora C18 column ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were routinely passaged every 2-3 days by washing twice with PBS lacking divalent cations and treating with StemPro Accutase (ThermoFisher A1110501).
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bioRxiv - Microbiology 2024Quote: ... Plates were placed in sealed boxes to prevent from drying of the perimetric wells and incubated without shaking at 37°C for 6 days before addition of a mix solution of 20% tween 80 plus MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (Stock 5 mg/mL, Acros Organics, Ref. 15224654) or the Bactiter-Glo Luciferase Assay System (Promega ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 7376 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... The grids were blotted for 3 seconds at 100% humidity with force “2” and plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). 41335 movies were recorded on a 300 keV Titan Krios microscope (Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... and incubated in total of 150 µL SH2 binding buffer containing 20 µM biotinylated SH2 domain on a rotator and 4 °C for 2-3 hours in low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™). The functionalized magnetic beads are then washed twice in 1 mL of SH2 binding buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... with an in-line Acclaim PepMap 100 C18 trap column (75 μm × 2 cm, 3 μm, 100 Å, Thermo Fisher Scientific) heated to 45 °C ...
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bioRxiv - Cell Biology 2024Quote: ... were plated onto 6-well culture plates at 2×105 cells/well (concentration determined using the Countess 3 automated cell counter; Invitrogen: AMQAX2000) for downstream experiments (n=5 biological replicates/treatment) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were acidified to pH 2-3 using trifluroacetic acid (TFA) prior to LC-MS analysis on a Dionex U3000 UHPLC system (ThermoFisher Scientific) equipped with a column chromatography setup coupled to a Q Exactive Plus Orbitrap MS (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown to a confluency of 70-90% and passaged every 2-3 days via trypsinization with 0.05% Trypsin-EDTA (25300-054, Thermo Fisher Scientific). Cell lines were regularly checked for Mycoplasma contamination and confirmed negative ...
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bioRxiv - Genetics 2024Quote: ... Slides were then washed for 2 minutes in PBS 3 times then incubated for 2 hours with goat anti–rabbit IgG/Alexa Fluor 594 secondary antibody (Thermo Fisher Scientific A-11012 at 1:1000) in blocking buffer at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... the inoculum was removed, and 2 mL overlay media was applied (MEM with 10% FBS containing 1% methylcellulose, 1% HEPES, 1% GlutaMAX (Gibco), and 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Microbiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) at 1 μg mL−1 concentration and 50 μL of Film Tracer 1-43 membrane marker (Invitrogen) at 1 μg mL-1 concentration in DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were transfected at a concentration of 106 cells ml-1 with plasmid DNA (1 mg l−1) and 25 kDa linear polyethyleneimine (2 mg l−1) in OptiPRO SFM (Gibco). After 6 d of culture ...
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bioRxiv - Cell Biology 2024Quote: ... plates were prepared with 2 % agar in YPD (1 % yeast extract, 1 % peptone, and 1 % glucose) supplemented with 100 μg/mL zeocin (Invitrogen), 300 μg/mL hygromycin B (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... six-well plates containing prewarmed neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% Glut Amax, 1% P/S; all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... the spheroids were transferred to non-adhesive 100 mm dishes containing Neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% GlutaMAX, 1% P/S all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Immunology 2024Quote: Isolated LECs were placed in complete T-cell media (RPMI + 10% FBS + 1% L-Glu + 100U/ml Pen/Strep + 1% MEM NEAA + 1% Na-Pyruvate + 0.1% 2-mercaptoethanol) (Gibco, USA) with PBS or LPS (1µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... or a combination of pepsin and fungal protease type XIII (manually conjugated to POROS™ 20 AL Aldehyde Activated Resin, Thermo Fisher Scientific, and packed in a 2 mm ID x 2 cm IDEX C-130B column, Fisher Scientific, Hampton, NH), loaded on a C18 analytical column (Hypersil Gold ...