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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and finally coated with 1-2 μg/ml laminin in Neurobasal media (NB media, Gibco) with 2% PSF at 37°C for 4 hours.
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bioRxiv - Bioengineering 2021Quote: ... Caspase 3/7 assay solution was mixed 1:1 with αMEM without phenol red (ThermoFisher Scientific) (caspase 3/7 lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... 200μL of platelet suspension (2×106 platelets in HBSSCa-Mg-) from healthy donors was labelled with calcein-AM (2 μg/mL final concentration, ThermoFisher Scientific Cat # C3100MP) for 15 mins and then R406 ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Developmental Biology 2023Quote: ... we washed the mature COCs in synthetic oviductal fluid medium (SOF) containing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES-TL, Thermofisher Scientific, Waltham, MA) and SOF for fertilization (SOF-FERT ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Microbiology 2022Quote: ... and the residual bone marrow cells were plated in tissue-culture-treated microplates at a density of 2 × 105 cells per well in RPMI 1640 containing 2 mM glutamine (Thermo Fisher Scientific, 21875034), 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: To assess endothelium barrier function we perfused microvessels with 2 μM Alexa Fluor647-conjugated 2 MDa dextran (Thermo Fisher Scientific, cat. no. D22914) in EGM-2 medium 48 h following inoculation with Bb or vehicle (BSK-II medium ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... and SARS-CoV-2 S protein were immobilized at 2 μg/ml on 96-well polystyrene microtiter plates Nunc MaxiSorp™ (Thermo Fisher Scientific). Following blocking with 1 × synthetic block (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were concentrated and washed onto a trap column (75 μm × 2 cm, 2 μm C18 resin, 100 Å; Acclaim PepMap100, Thermo Fisher Scientific) for 5 min with 2% ACN and 0.05% aqueous TFA at a flow rate of 30 µl/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Dako (cat # P026002-2 and cat # P021702-2) and detected using either SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, cat # SKU:34579) or Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate (Perkin Elmer ...
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bioRxiv - Developmental Biology 2024Quote: ... The pellet was then resuspended in 2 mL of MACS buffer (Hanks Buffered Salt Solution, 2% FBS, 2 mM EDTA) and incubated with streptavidin coated magnetic beads (Invitrogen MSNB-6002-74), spinning for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting peptic peptides were collected on a trap column (2 mm × 2 cm) that was filled with POROS 20 R2 material (Thermo Fisher Scientific, USA) and kept at 0.5 °C ...
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bioRxiv - Cell Biology 2024Quote: ... placed into individual wells in a 12 well suspension culture plate (greiner bio-one Cellstar, #665 102) containing warmed, oxygenated resting media (2 ml, RPMI 1640 with 2%FBS (GIBCOTM ThermoFisher Scientific, Cat# 11875093) and incubated overnight while on an orbital shaker.
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Immunology 2022Quote: ... and Cell Event Caspase-3/7 Green (Invitrogen) were loaded at the final concentration of 5 µM and 7 µM respectively along with the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase-3 and −7 Cell Imaging Kit (Invitrogen), and Propidium iodide (PI ...
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bioRxiv - Systems Biology 2024Quote: ... CellEventTM Caspase 3/7 detection reagent (Invitrogen C10423), and SYTOXTM AAdvanced Dead Cell Stain (Invitrogen S10349) ...
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bioRxiv - Physiology 2024Quote: CellEvent Green Caspase 3/7 dye (ThermoFisher Scientific) was added to cells in a glass bottom plate with +/- 3-oxo in phenol-free DMEM ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells were inoculated with 10-fold dilutions of SARS-CoV-2 for 1 h and overlaid with 0.8% (w/v) Avicel medium (1 x MEM Temin’s modification (Gibco), 0.8% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The HDFC was dissolved with a 1:1 mixture of 2 mg/ml collagenase type2 and trypsin (Gibco), and cell numbers were quantified using hemocytometry ...
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bioRxiv - Neuroscience 2022Quote: ... and then switched to neural induction medium (Advanced DMEM/F12 (1:1) supplemented with 2 mM GlutamaxTM (Gibco), 2% B27 without vitamin A (Life technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... were similarly cultured but in DMEM:Ham’s F12 (1:1) medium with 2 mM glutamine and 10 % fetal-calf serum (Invitrogen). FugeneHD (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Thawed CM aliquots were mixed 1:1 with basal media (BM) consisting of 2% B-27 (Gibco 12587001), 10 mM Nicotinamide (Sigma-Aldrich N0636) ...
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bioRxiv - Neuroscience 2024Quote: ... Wisteria floribunda lectin (1:1000, B-1355-2, Vector) was visualized using Streptavidin 568 (1:500, S11226, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... mixing with 2 million CD45.1+ T cells at 1:1 ratio and labeling with CellTrace Violet dye (Invitrogen), into Rag1−/− mice ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 1 minute in 1 ml PBS 2% formaldehyde (Thermo-Fisher Scientific, Cat. No. 28906) before being fixed for a further 14 minutes in fresh PBS 2% formaldehyde ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended at 2 x 106 cells/ml in assay medium containing DMEM/F12 (1:1, Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and plated 1×106 and 2×106 cells on Geltrex (Gibco, 1:100 DMEM/F12, Thermo Fisher Scientific)- coated 60 mm and 10 cm plates respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock solution, Invitrogen S32357) was added ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly isolated MNCs were washed and re-suspended in PBS and stained for 10 min at 37°C with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) at a concentration of 5 µM (Thermo Fisher Scientific, Waltham, MA, USA). H2DCFDA is a non- fluorescent dye which is cleaved inside cells to 2’,7’-dichlorofluorescein (H2DCF) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with 3 mM dimethyl 3,3′-dithiobispropioimidate (DTBP; Thermo Fisher Scientific, #20665) for 5 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was injected onto an EASY-Spray PepMap C18 column (75 mm id 3 25 cm, 2 mm particle size) (Thermo Scientific) and separated over a 2-h method ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...