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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Islets were cultured overnight (37°C, 5% CO2) in RPMI1640 medium (ThermoFisher, Waltham, United States) with 11 mM glucose (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µL of 1 mg/mL Escherichia coli Bioparticles AlexaFluor-594 conjugate (ThermoFisher cat# E23370) were injected directly below the sealed wound at 3 dpi using a Hamilton syringe while fish were anesthetized ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH reverse: 5′-AAG TGG TCG TTG AGG GCA ATG -3′ (Invitrogen, Thermo Fisher Scientific); ALIX forward ...
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bioRxiv - Bioengineering 2024Quote: ... GAPDH reverse: 5′-AAG TGG TCG TTG AGG GCA ATG -3′ (Invitrogen, Thermo Fisher Scientific); ALIX forward ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µm thick sagittal sections were cut using a microtome (ThermoFisher Scientific, Waltham, MA, USA) and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Bioengineering 2024Quote: ... was coated with 1 mL of 5% Pluoronic F-68 (Gibco, Waltham, MA, USA; 24040032) and incubated for 30 minutes at room temperature before replacement with PBS for storage ...
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bioRxiv - Bioengineering 2024Quote: ... MCF10A-5E cells70 were cultured in DMEM/F12 supplemented with 5% horse serum (Invitrogen,16050122), 20 ng/mL EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 5 minutes at 37°C with 1 mL room temperature Accutase (Gibco) to detach the cells from the plate ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and the cells resuspended in 3-5 mL of pancreatosphere medium (DMEM/F12 (Gibco, #11320033), 1% P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% Bovine Serum Albumin (BSA) (Fisher Scientific) dissolved in 1X Tris-Buffered Saline with 0.01% Triton-X-100 (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM imidazole) and incubated with 500 µl Ni-NTA beads (Thermo Fisher Scientific, 88831) at 4 °C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... cells were treated with 5 µM of Cell Trace Violet Cell Proliferation Kit (C34557; ThermoFisher), incubated for 10 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Immunology 2024Quote: ... frozen in 5 × 106 cells/ml aliquots in 90% FBS/10% DMSO (Thermo Fisher Scientific) and stored at –80°C prior to being shipped on dry ice to New York ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were cultured at 37°C in 5% CO2 using DMEM (Gibco, 41965-062) supplemented with 10% heat-inactivated Tet-free fetal bovine serum and 1% penicillin/streptomycin (Gibco 15070063) ...
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bioRxiv - Immunology 2024Quote: ... Vybrant CFDA SE Cell Tracer Kit-Carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen, V12883, 5 µM); CellTrace Violet (CTV ...
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bioRxiv - Neuroscience 2024Quote: ... cells were stained with Vybrant™ DyeCycle™ Green Stain (5 µM, V35004, Thermo Fisher) after completing the Cell Mito Stress Test or Glycolysis Stress Test ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM MgCl2) using ZebaTM Spin Desalting Column with a 7k MWCO (Thermo Fisher Scientific). A serial dilution of the protein samples was prepared in a black 384-well non-binding microplate (Greiner Bio-One ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 30 minutes with streptavidin-PE (5 µg/mL, Thermo Scientific #12-4317-87). Results were read on a Luminex xMAP Intelliflex system.
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bioRxiv - Physiology 2024Quote: ... and donkey anti-rat DyLight 550 (5 µg/mL, Cat. # SA5-10027; Invitrogen, Darmstadt, Germany) for 1 hour followed by incubation with Hoechst (2 µmol/L ...
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bioRxiv - Physiology 2024Quote: ... donkey anti-mouse Alexa Fluor 647 (5 µg/mL, Cat. # A-31571; Invitrogen, Darmstadt, Germany), or donkey anti-rabbit Alexa Fluor 555 (6.67 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were cultured at 37°C and 5% CO2 in McCoy’s 5A medium (Gibco) supplemented with 10% FBS and 100 U/ml penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... coverslips were incubated for 5 min at room temperature with Hoechst 33342 (Life Technologies H3570) diluted 1:1000 in double-distilled water to stain cell nuclei ...
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bioRxiv - Genetics 2024Quote: ... as internal size standard and results were processed by GeneMapper™ Software 5 (Applied Biosystems). The fragment sizes were converted to HTT CAG repeat numbers by comparison to predetermined HTT CAG repeat numbers of standard DNA (Standard Reference Material® 2393 ...
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bioRxiv - Cell Biology 2024Quote: ... L4 worms were sterilized by 5-fluorodeoxyuridine treatment (CAS number 50-91-9, Acros organics), and lifespan was measured by testing the worm movement with gentle poking using a worm pick ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated in 5× SSC-T buffer supplemented with 1 μg/mL DAPI (Invitrogen) for 20 min at room temperature with gentle rocking ...
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bioRxiv - Plant Biology 2024Quote: ... Cells were disrupted by passing them 3-5 times through a French press (Thermo Fisher) and centrifuged for 50,000g for one hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was run on a QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific) using the Maxima SYBR Green/ROX qPCR Master Mix (2X ...
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bioRxiv - Cancer Biology 2024Quote: ... Treated cells were then counterstained with DAPI for 5 minutes and ActinGreen 488 (Thermofisher, R37110) for 20 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: ... 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer ...
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bioRxiv - Neuroscience 2024Quote: ... then an IP injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU (ThermoFisher Scientific)) 24 h later and were perfused after a further six days.
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bioRxiv - Microbiology 2024Quote: ... Tol 5 whole genome database (AP024708, AP024709; NCBI) were performed using SEQUEST (Thermo Fisher Scientific). The search parameters were set as follows ...
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bioRxiv - Microbiology 2024Quote: ... and/or blood agar (BA, tryptic soy agar with 5% sheep blood, Thermo Fisher Scientific). Note that both PTA and BA plates contain heme precursors ...
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bioRxiv - Microbiology 2024Quote: ... parasites were rinsed in PBSTx then permeablized in 5 μg/ml proteinase K (Ambion AM2546). Following proteinase K treatment ...
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bioRxiv - Microbiology 2020Quote: ... A two-step qRT-PCR reaction in triplicate was performed on a Quantstudio 5 (Applied Biosystems). Reverse transcription was carried out with the Superscript III RT first strand synthesis kit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... T cells were maintained at 37°C and 5% CO2 in RPMI 1640 media (Life technologies) supplemented with 10% FBS (MERCK) ...
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bioRxiv - Biophysics 2021Quote: ... about ~5 μg of bacmid were transfected using 6 μl of CellfectinII reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
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bioRxiv - Genetics 2021Quote: ... In vitro transcription of 5’ capped mRNAs was performed using the mMACHINE SP6 Transcription Kit (Ambion) following manufacturer’s protocol and as previously described in (33 ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto the trapping column (PepMap100, C18, 300 µm x 5 mm, Thermo Scientific) using partial loop injection with 2% acetonitrile (ACN) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 mM Sodium Citrate (pH 5 at 25°C) and 0.2 U/µl RNase T1 (ThermoFisher). Reaction mixtures were incubated at 50°C for 10 min prior to being stopped by flash freezing in liquid nitrogen for 5 min and adding equivolume 90% formamide ...
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bioRxiv - Systems Biology 2020Quote: ... Disulfide bonds were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 hr ...
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bioRxiv - Systems Biology 2020Quote: ... cells were incubated with 5 μg/mL HRP conjugated goat-anti-rabbit (Thermo Fisher Scientific; A16110) in 1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... protected by an Acclaim PepMap C18 column (100 μm x 2 cm, 5 μm; Thermo Scientific) before injection into a Q-Exactive mass spectrometer (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial ROS was detected by incubating cells with 5 μM Mito-Sox Red (Invitrogen, Carlsbad, CA) for 10 min at 37 C and measuring fluorescence using a microplate reader.
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bioRxiv - Cell Biology 2020Quote: ... using 5 μl/ml Lipofectamine RNAiMAX diluted in Opti-MEM I Reduced Serum Medium (Life Technologies). The cultures were analysed on day 6 of differentiation.
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bioRxiv - Cell Biology 2020Quote: ... A375 cells were cultured at 37 °C with 5% CO2 in DMEM (Thermo Fisher, #12800-082) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with anti-cytochrome C (33-8200, 5 μg/ml, mouse monoclonal, Thermo Fisher) and anti-SPIRE1 (SA-2133 ...
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bioRxiv - Cell Biology 2019Quote: Cells were incubated at 37°C and 5% CO2 with MitoTracker-Red FM (100 nM) (Invitrogen) for 15 min to stain mitochondria and Hoechst 33258 (2.5 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... adapter-ligated template was digested with 5 U of AmpErase Uracil N-Glycosylase (Life Technologies, N8080096). Libraries were then PCR amplified and indexed using Phusion Hot Start II High Fidelity Polymerase (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ATCC HeLa CCL2 (ATCC) were cultured at 37°C and 5% CO2 in DMEM (Gibco 11965092) supplemented with 10% fetal bovine serum (Gibco ...