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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The protein concentration was measured on the cleared lysates with PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After diluting the lysates in RIPA and Laemmli buffer ...
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bioRxiv - Plant Biology 2019Quote: ... The supernatant was filtered through a 0.22 μm Millipore Express PES membrane and proteins were quantified using PierceTM BCA protein assay kit (Thermo Fisher Scientific). Small aliquots were taken as input samples whereas the remaining parts were used for co-IP ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in 1x Laemmli Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23250; ThermoFisher Scientific). Proteins (10µg/sample ...
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bioRxiv - Cancer Biology 2019Quote: ... mAb-phage complexes were captured by magnetic beads (20µL of protein A-coated and 20 µL of protein G-coated, catalog numbers 10002D and 10004D, Invitrogen, Carlsbad, CA) for 4 hours at 4°C with rotation and processed using the Agilent Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was transferred to a new tube for protein quantification with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23225). 40 μg of protein was separated by NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations of the solutions were measured using a bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Supernatant (2 mg of proteins) was incubated with 2 μg of target antibodies and 20 μl of Protein A/G Plus Ultralink Resin (Thermo Scientific) for 4 hours at +4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: Expression of BRCA2 at protein level was confirmed by western blotting using NuPAGE® Large Protein Blotting Kit (Invitrogen, CAT# LP0001) following the protocol supplied by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified protein quality was assessed by SDS-PAGE/Coomassie staining to be >95% pure and protein concentration was quantified with PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). Protein was stored at - 80°C until further use.
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bioRxiv - Biochemistry 2020Quote: ... and lysates containing 30 µg of protein were electrophoresed on a NuPageTM 4–12% Bis-Tris Protein gel (Thermo Fisher Scientific). Proteins were transferred to a PVDF membrane (Amersham) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cytokine measurements for lung homogenate samples were corrected for total protein concentration using a Pierce™ BCA Protein Assay Kit (ThermoFisher).
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Microbiology 2020Quote: ... The total protein concentration in each sample supernatant was quantified using the bicinchoninic acid (BCA) method (Pierce BCA Protein Assay Kit; Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: Conventional interactome analysis of the IP samples was performed as described before [57] with the following changes: The enriched proteins were separated by SDS-PAGE with a 4-12% Bis-Tris protein gel (NuPAGE, Thermo Scientific) and stained with Colloidal Blue Staining Kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was measured with the BCA Protein Assay Kit (Pierce Biotechnology) and equal amounts of protein were separated on 4–12% Tris-Glycine Gels (Thermo Scientific) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration was determined using a bicinchoninic acid (BCA) assay from the Pierce™ BCA Protein Assay Kit (Thermo Scientific). The fluorescence spectra of the EYFP samples were measured with a Varioskan LUX Multimode Microplate Reader (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...
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bioRxiv - Cell Biology 2019Quote: ... The protein concentration of the conditioned medium was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, cat#23225), and medium volumes ...
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bioRxiv - Neuroscience 2019Quote: ... TAG concentrations were normalized to the total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). Each assay was done in triplicate and the average TAG level (μg TAG/ μg protein ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was also snap frozen and stored for protein analysis via the manufacturer’s instructions for the Pierce BCA Protein assay kit (Thermo Fisher Scientific) to normalize the NMR data ...
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bioRxiv - Biochemistry 2020Quote: ... The microsomes were resuspended and diluted according to the protein concentration measured in the supernatants using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Genetics 2021Quote: ... Then concentration of the protein was measured by the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA, Cat. #23227). Samples were stored at -80°C until proteomic preparation.
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bioRxiv - Neuroscience 2020Quote: ... Assay results were normalized to the total protein level in each ON sample as quantified with the Pierce BCA protein assay kit (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation of protein extracts was performed using a standard protocol based on magnetic beads coupled to bacterial protein G (Invitrogen #10003D). 1µg of anti-LRRFIP2 antibodies per 500µg of protein extracts was used ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... The optical density at 492 nm was measured to estimate melanin content and normalized to protein concentration as determined by BCA protein determination kit (Thermo Fisher). Plat-E cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates from 30 wt eye imaginal discs (1 mg of total protein) was precleared by incubating with protein A-sepharose beads (Thermo Scientific) for 1h at 4 °C followed by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of protein crude extract was determined by means of bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the lysates was measured via BCA assay and biotinylated proteins were immunoprecipitated using Streptavidin magnetic beads (Thermo Fisher). 500μg magnetic beads were incubated with up to 70μg of lysate rotating overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2021Quote: Total protein of 40 μg was prepared from each sample and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher). We ran the protein in sodium dodecyl sulfate-polyacrylamide (SDS ...
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bioRxiv - Genomics 2020Quote: ... the protein concentration of lysates was first determined using the Microplate BCA protein assay kit – reducing agent compatible (Thermo Fisher Scientific), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The total protein concentration of each sample was determined using the Pierce(™) BCA Protein Assay Kit (Thermo Fisher Scientific Inc.) and the saliva sample was prepared with equal concentrations of proteins from each individual sample ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Genomics 2021Quote: Protein lysates derived from same plates used for metabolomics collection were assayed for total protein concentration with Pierce BCA Protein Assay Kit (ThermoFisher 23225) for normalizing raw metabolite values ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: LC-MS files were searched against Saccharomyces cerevisiae Swiss-Prot eIF4A protein sequence (Uniprot Protein Accession Number P10081) using Sequest HT embedded in Proteome Discoverer 1.4 (Thermo Fisher Scientific). Searching parameters include ...
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bioRxiv - Developmental Biology 2022Quote: ... and then normalized to both the internal standard (d5-2HG) peak area and the protein content of duplicate samples as determined by a BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Protein was extracted immediately after as described above and protein concentration was calculated following the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). A total of 600 µg were immediately used for the assay following manufacturer’s indications ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer (62.5 mM Tris-HCl (pH 6.8) ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-20 μg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration of the cell lysates was then determined using Pierce™ BCA Protein Assay Kit from ThermoFisher (#23225). After 100 mM DTT was added ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was transferred into a new tube and the protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). Protein concentration was adjusted to 0.5 µg/µL using lysis buffer and 45 µL were stored at −80°C (“input”) ...
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bioRxiv - Neuroscience 2022Quote: ... tissue debris was removed by centrifugation and protein concentrations were determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog # 23225). 10 μg of proteins per sample were resolved by SDS-PAGE and transferred onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and measured for a protein concentration using the BCA™ protein assay (Thermo Fisher Scientific, MA, USA). The protein sample was diluted with Laemmli buffer containing β-mercaptoethanol (Bio-rad ...
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bioRxiv - Neuroscience 2022Quote: Approximately 10 μg of synaptosome protein was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermo Fisher) and transferred to nitrocellulose membrane (AmershamTM Protran® Western Blotting Membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).