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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The final pellet was resuspended in 200 μL of extraction buffer and the mitochondrial protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). For the Proteinase K accessibility assay ...
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bioRxiv - Neuroscience 2022Quote: ... and human brain tissues were prepared using a RIPA buffer and the protein contents were measured using a PierceTM BCA Protein Assay Kit (Thermo Scientific). Proteins were separated on 12% SDS-PAGE gradient gel and transferred onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular debris was pelleted for 15 minutes at 4 °C and protein concentration was quantitated with the Coomassie (Bradford) Protein Assay (ThermoFisher Scientific). A total of 15μg (organoids ...
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bioRxiv - Microbiology 2022Quote: Our metabolic click-labelling was based on the Click-iT HPG Alexa Fluor protein Synthesis Assay Kit (Click-iT HPG Alexa Fluor Protein Synthesis Assay Kit, ThermoFisher C10428) strategy ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein synthesis was measured using Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10456; Life Technologies) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 4 μg of purified protein was mixed with DTT and analysed using a NuPAGE™ 4-12% Bis-Tris mini protein gel (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Protein concentration of control and stimulated myotube whole cell lysates was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following the manufacturer’s instructions as described before [37] ...
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bioRxiv - Bioengineering 2022Quote: ... OMV coating on AuNP was determined by quantifying the protein content in OMV using a CBQCA protein quantitation kit (ThermoFisher Scientific). For stability studies of OMV coated AuNP ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged and the supernatants were transferred to new tubes for protein quantification using the Qubit Protein Assay Kit® fluorometric (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatants were collected and the total protein concentration was determined by BCA protein assays (Thermo Scientific™ Pierce™, # 23225). The proteins (20-30 μg ...
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bioRxiv - Immunology 2022Quote: Homopropargylglycine (HPG) protein synthesis was measured using the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants from cerebral cortex neurons were centrifuged at 10,000 g and 4°C and subjected either to protein precipitation or to immunoprecipitation using Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific).
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Pathology 2022Quote: ... Cell lysates were centrifuged and the protein concentration of the supernatants were quantitated using BCA protein assay kit (Thermo Scientific, 23227). Cell extracts (800 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 60°C for 15 min and protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific). The protein mixture (30∼50 µg ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was determined with a BCA Protein Assay kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA). To each protein sample (20 μg) ...
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bioRxiv - Cancer Biology 2022Quote: ... Pull-down of RNA– protein complexes was performed using a Pierce™ Magnetic RNA-Protein Pull-Down Kit (20164; Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2022Quote: ... At least 400 μg of total protein were incubated overnight with the designated antibody and then with protein G-coupled magnetic beads (Life Technologies) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
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bioRxiv - Genetics 2022Quote: ... One μL from each sample was used to quantify protein amounts in using BCA protein assay kit (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Systems Biology 2022Quote: ... the protein concentration was determined using the Pierce BCA protein Assay kit in combination with a NanoDrop 2000 photospectrometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in 1% NP-40 lysis buffer (58) and protein levels were quantified using the Bicinchoninic acid protein assay (Thermo Scientific). Samples were run on SDS-PAGE gels before being transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... An RNA–protein pull-down assay was performed using a Pierce Magnetic RNA–protein pull-down kit (20164, Thermo Fisher Scientific). In brief ...
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bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
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bioRxiv - Neuroscience 2020Quote: ... Biochemical analyses were performed on water-homogenised tissues (50 mg/mL) and protein content determined (BCA protein assay, Thermo Fisher #23227) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The amount of total protein in each lane was determined by using the No-Stain Protein Labeling Reagent (Thermo Fisher Scientific). Imaging for puromycin and total protein signals was performed on a FUSION FX7 EDGE Imaging System (Witec AG) ...
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bioRxiv - Microbiology 2020Quote: ... Both viral RNA and protein were isolated from these PBMCs at the same day using Ambion Paris RNA and protein extraction kit (ThermoFisher Scientific) and stored in −80 °C until use ...
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bioRxiv - Microbiology 2020Quote: ... 20 min) and the total protein concentration of the cleared lysates was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Luminescence readings for each sample were compared to an ATP standard curve and normalized to the total amount of protein determined using the Pierce BCA Protein Assay Kit (23225, Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and the protein assay was performed using the Pierce BCA Protein Assay kit (Cat#23225, ThermoFisher Scientific). The supernatants from different samples were adjusted to the same protein concentration of protein and the same volume of the supernatants were subjected to acetylcholine assay using QuickDetectTM Acetylcholine (ACh ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 200–300 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 60–100 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Physiology 2021Quote: ... protein content was measured from 100 μl of diluted samples with Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, IL, USA) and lipid content was analyzed by hydrolysis according to an internal method adapted from NF ISO 6492 by Artemis Laboratory (Janzé ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Loughborough, UK). Working solutions were made up to 0.5 μg/μl with RIPA and 2x Concentrate Laemmli Sample Buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: The whole extract and the cytosolic and synaptosome protein fractions were collected using the Syn-PER™ Synaptic Protein Extraction Reagent (ThermoFisher) following manufacture instructions ...
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bioRxiv - Immunology 2020Quote: ... Punch biopsy skin samples were obtained from XenoSCID and protein extracted and homogenised using M-PER mammalian protein extraction reagent (Thermo Scientific) and two 7mm metal beads ...
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bioRxiv - Neuroscience 2021Quote: ... PS2 or PSD fractions were quantified for protein concentration using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA).
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bioRxiv - Biochemistry 2021Quote: ... The purity of the protein was checked by SDS-PAGE and the concentration was determined using a Pierce BCA protein assay kit (Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... subcellular protein fractionation was performed as described in the Subcellular protein fractionation kit for cultured cells (Thermo Scientific; Cat. No. 78840). Fractions were stored in −80°C per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Immunology 2022Quote: ... The Protein G resin was washed four times with PBS and the IgG was eluted with Protein G Elution Buffer (Thermo Scientific) and neutralized with 1 M Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Data were analyzed using the Agilent Seahorse Wave Software and normalized to total protein context per well using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Physiology 2022Quote: ... 20 mg of pulverized tissue were dissolved in the assay buffer from the kit as suggested by the manufacturer and normalized to protein content by Pierce™ BCA Protein Assay Kit (ThermoFisher). 250 μg of protein was used for each reaction ...
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bioRxiv - Biophysics 2019Quote: ... Total protein concentration of the extracts was determined using the Pierce™ Coomassie Protein Assay Kit (ThermoFisher Scientific, Porto Salvo, Portugal).
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Molecular Biology 2019Quote: ... with the addition of 30 µl of protein A (for anti-H3K9me) or protein G (for anti-FLAG) Dynabeads (Thermo Fisher). Library preparation was performed as described in (Homer et al. ...
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bioRxiv - Biochemistry 2020Quote: Protein concentration in the cell extract was measured using a Pierce BCA protein assay kit (product 23227; Thermo Scientific, Rockford, IL).
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bioRxiv - Evolutionary Biology 2019Quote: ... and each sample was standardized to protein concentration determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).