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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: HEK-293Ts transfected with the indicated plasmids were lysed in M-PER lysis buffer (Thermo Scientific) supplemented with EDTA-free protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: Cells were transiently transfected with the expression vector plasmids using electroporation (Neon Transfection System, Life Technologies) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PCR and insertion into Zero Blunt TOPO PCR Cloning Plasmid (Thermo Fisher Scientific, 450245) which were transfected and expanded per manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA molecules and plasmid were transiently transfected into hESCs using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated plasmid (1 µl) was then transformed into One Shot BL21 Star (DE3) competent cells (Invitrogen) with outgrowth in SOC media for 1 hour at 37°C before plating on LB agar plates containing kanamycin (50 µg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligation of this fragment into the pX330 modified plasmid was performed using T4 DNA Ligase (Invitrogen) in an overnight reaction at 16°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were transfected with 10 µg of plasmid DNA (Table S3) and cultured under Puromycin (Gibco) selection (5 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... VSMCs at 70-80% confluence were transfected with indicated plasmids using TransIT-LT1 reagent (Life Technologies). The transfection efficiency of this method was approximately 60%.
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bioRxiv - Neuroscience 2023Quote: ... Luciferase reporter plasmid DNAs or in vitro transcribed RNAs were transfected by using Lipofectamine 3000 (ThermoFisher) according to the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 1.5 μg of transgene plasmid was prepared in 37.5 µL of OptiMEM (Thermo Fisher Scientific). This mix was combined with 9 μL of TransIT-LT1 (Mirus ...
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bioRxiv - Physiology 2023Quote: ... Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... expression plasmid constructs were introduced into CGNs by Neon electroporation according to manufacturer instructions (Life Technologies). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of plasmid DNA was mixed with 4 µL of Lipofectamine 2000 (ThermoFisher Scientific #11668019) in 300 µL of Opti-MEM without any additives ...
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bioRxiv - Microbiology 2023Quote: Plasmid transfections were performed using Lipo2000 transfection reagent (cat no. 11688-030, Invitrogen by ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: Plasmid transfections were performed using Lipo2000 transfection reagent (cat no. 11688-030, Invitrogen by ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested plasmid and DNA fragment (if necessary) were ligated with T4 DNA ligase (Thermo Scientific) or One Step Cloning Kit (YEASEN ...
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bioRxiv - Molecular Biology 2023Quote: ... empty vector control + PPARγ or PPARγ + ZFP407 plasmids using Lipofectamine 3000 (ThermoFisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... Phage DNA was extracted from the pellet using a GeneJET Plasmid Miniprep kit (Thermo Fisher, #K0502) and analyzed by Sanger sequencing.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA of mtRF1-AAG-3xFLAG in a pcDNA3.1 vector backbone was ordered from Thermo Scientific. DNA and PEI at a ratio of 1:3 (w/w ...
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bioRxiv - Cancer Biology 2023Quote: ... The purity and concentration of each plasmid were assessed with a NanoDrop™ Lite Spectrophotometer (Invitrogen). Two days before transfection ...
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bioRxiv - Cell Biology 2023Quote: ... plasmids and siRNA were transfected in mammalian cells at 70% confluency using Lipofectamine 3000 (Invitrogen, #L3000001). PLK4 siRNA and CEP152 siRNA oligonucleotides (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Gibson assembled plasmids were then transformed into Multishot™ TOP10 Chemically Competent E.coli (Invitrogen™ #C4081201) cells using manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The amplified DNAs were inserted into the multiple cloning sites of plasmid pCDNA3.1 (+) (Thermo Fisher Scientific). To synthesize RNA ...
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bioRxiv - Cell Biology 2023Quote: ... Subconfluently cells were transfected using 2 µg/ml of plasmid and Lipofectamin 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... capped RNA was generated from the pToto1101-nsP3-mScarletI plasmid (Invitrogen mMessage mMachine SP6 kit, AM1340) and electroporated into BHK-J cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The newly synthesized plasmids were transfected into DH5-alpha competent cells (Thermo Fisher Scientific, MA, USA), and the transfected cells were plated on LB agar plates containing ampicillin for selection ...
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bioRxiv - Genomics 2024Quote: ... GUS control plasmid was provided as part of the LR Clonase II enzyme kit (Invitrogen 11791020). Lentiviral supernatants were collected at 24 and 48 hours and concentrated via spin dialysis ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were transfected with 500 ng of plasmid DNA using Lipofectamine 2000 (Thermo Fisher Scientific) and following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... the cloned reporter plasmids and the control pEGFP-N1 were co-transfected by Lipofectamine 3000 (Invitrogen) at a ratio of 3:1 (2.5 μg in total) ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmid was then transfected into MCF-7 cells using the Lipofectamine LTX (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... A CMV expression plasmid encoding Sumo-HRV-CD19-Spytag-Histag was transfected into Expi293F Cells (ThermoFisher) using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Expression plasmids were transfected into the ExpiCHO cell lines according to the manufacturer’s protocol (Thermo Fisher). Supernatant containing monoclonal antibodies were clarified by centrifugation (1,400g ...
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bioRxiv - Cancer Biology 2024Quote: ... A3BWT and A3BE255Q sequences (cDNA plasmids gift from John Maciejowski) were cloned into pDONR221 (Invitrogen 12536017) using the Gateway BP Clonase II Enzyme mix (Invitrogen 11789020) ...
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bioRxiv - Developmental Biology 2024Quote: ... pHLsec-Tsg-TwinStrep and pHLsec-CHRDL2-TwinStrep plasmids were transfected to Expi293-F cells (ThermoFisher Scientific) using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... both plasmids containing sgRNAs were transfected into naïve HEK293 cells by Lipofectamine 3000 (Thermo Fisher Scientific) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmid was introduced by heat shock into DH5alpha and extracted with standard Miniprep (#K0503, ThermoFisher). The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521 ...
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bioRxiv - Cell Biology 2024Quote: ... The three plasmids were transfected together into keratinocytes using Lipofectamine LTX with PLUS reagent (Invitrogen #A12621) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 70%-80% confluent monolayer of cells was transfected with various plasmid constructs using Lipofectamine 2000 (Invitrogen) and Turbofectamine in Opti-MEM (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: ... the pA16-GFP plasmid was linearized by incubation with the restriction enzyme EcI136II (EcoICRI, Thermo Scientific) at 10 U/μ purification using the QIAprep Spin MINI Prep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pVSV-G (390 ng) packaging plasmids in 5 × 105 HEK293T cells with Lipofectamine 2000 (Invitrogen). The supernatant was collected 40 hours later ...
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bioRxiv - Genomics 2024Quote: ... which was purified using PureLink™ Endotoxin-Free Maxi Plasmid Purification Kit (ThermoFisher Scientific, cat. #A31231) to obtain 2 mg of the final focused STARR-seq plasmid library ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were transfected into HEK293T and HeLa cells using jetPEI (Polyplus transfection) or LipofectamineTM 3000 (Invitrogen), and siRNAs were transfected into HeLa cells using LipofectamineTM 2000 ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were first transformed into One Shot TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific; #C404003) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HepG2 Cells were transfected with the sgRNA plasmid for Hsp90α with Lipofectamine 3000 (Invitrogen, CA, USA). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids into HEK293F was performed using polyethyleneimine (PEI) diluted in Opti-MEM (Gibco). After 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... STAT6 and NOS2 plasmids were co-transfected with psPAX2 and pMD2.G with Lipofectamine 3000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Omp25 IVT optimized plasmid was transcribed via T7 polymerase in the presence of RNase inhibitor (Invitrogen) for 1 hour at 37° C ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus transfer plasmid was constructed from (pLenti6/V5-DEST™ Gateway™ Vector, Thermo Fisher Scientific) as backbone plasmid and entry plasmid (pENTR-H2B-EGFP ...
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bioRxiv - Microbiology 2024Quote: ... DENV RNA was transcribed in vitro from pACYC-DENV2-NGC plasmid using mMessage/mMachine kits (Ambion) and electroporated into BHK-21 cells ...
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bioRxiv - Neuroscience 2024Quote: ... 5.2 μg of the plasmid of interest and 880 µl of Optimem (Gibco, No. 31985-070). Plasmids were mixed ...