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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 20 μg of heavy and light chain expression plasmid DNA were diluted in OptiMEM (Life technologies) media containing polyetheleneimine (PEI ...
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bioRxiv - Biophysics 2022Quote: ... The expression plasmids were transiently transfected by using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and corresponding control plasmid were used to transfect Caco-2 cells using Lipofectamine 2000 (Invitrogen, 11668027) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Stable cell lines were created by electroporation of plasmids using a Neon Transfection system (Thermo Fisher) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by DNA purification from 12 dishes of transformants (PureLink HIPure Plasmid Filter Maxiprep Kit, Invitrogen). Lentivirus was prepared by transient transfection of 293FT (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA was generated from linearized plasmid using an SP6 mMessage mMachine Transcription Kit (Thermo Fisher Scientific) and column-purified using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... 1μg DNA of each plasmid construct were added and electroporated using the Neon transfection system (Invitrogen) following the manufacturer’s instructions (1200 volts ...
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bioRxiv - Cell Biology 2022Quote: All plasmids were transcribed into mRNA using the SP6 or T7 mMessage mMachine kit® (Invitrogen) and used for microinjections ...
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bioRxiv - Microbiology 2020Quote: ... the sequence of the plasmid EGFP-N was confirmed by sequencing (Gene Art–Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... pCAGGS(+)/GP or the mentioned empty plasmids as vector and 10 μl of lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: The plasmids were sequenced (Genetic Analyzer, ABI PRISM Model 3100, Applied Biosystems, Foster City, CA, USA) using the dideoxynucleotide method (Sanger et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids were added into a sterile tube containing 500 μl of Opti-MEM® I (Invitrogen). In a separate tube ...
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bioRxiv - Immunology 2020Quote: ... inhibitors and control mimics/inhibitors and/or plasmids was performed with Lipofectamine 2000 or 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Retrovirus plasmids were transfected into 293T-Eco cells using Lipofectamine 2000 (Thermo scientific, Cat. No. 11668019). 48 hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with relevant plasmids or siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific), and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... sub-cloned into plasmid pCR®2.1-TOPO® using the TOPO TA cloning kit (Invitrogen) prior to sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid DNAs and siRNAs were transfected using Jet Prime (Polyplus Transfection) and Oligofectamine (Thermo Fisher Scientific) reagents respectively as per manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmids were transfected into HEK293 human embryonic kidney cells using Lipofectamine2000 (Invitrogen, Carlsbad, CA, USA). The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220 ...
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bioRxiv - Biochemistry 2020Quote: NPC1−/− cells were transfected with wild type or mutant NPC1 plasmids with Lipofectamine 3000 (Life Technologies) according to the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5X105 cells were transfected with 1 μg of each plasmid using TurboFect transfection reagent (Thermo Scientific). Medium was replaced after 24 hr ...
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bioRxiv - Genetics 2022Quote: ... HEK293 cell cultures were transfected with 15 μg of the plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into the HindIII and XhoI sites of the pcDNA3.1(+) plasmid (Invitrogen, Carlsbad, CA, USA). The C-terminal 10× His-tagged NSP12 gene ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were heat shocked into Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... The three combined plasmids were diluted in 100 uL Opti-MEM Reduced Serum Medium (ThermoFisher 11058021). In parallel ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... the plasmid was linearsied using Dra I and transcribed using the Megashortscript T7 kit (Invitrogen, AM1354). Capped RNA encoding Cas9 was synthesised from XbaI-linearised pT3TS-nCas9n plasmid using the T3 mMessage mMachine Kit (Ambion) ...
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bioRxiv - Cell Biology 2019Quote: ... the plasmid coding for the Streptococcus pyogenes Cas9 and the free PCR product using Lipofectamin2000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmids were transfected into HCT116 Trisomy 5 cells using Lipofectamine 3000 (ThermoFisher, Cat. No. L3000001). The cells were then selected with puromycin for 2 weeks and then bulk-sorted with FACS for RFP-expressing cells ...
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bioRxiv - Immunology 2019Quote: ... Custom synthesized (IDT biotech) human KCNJ15 cDNA was sub-cloned into the PE1A plasmid (Invitrogen, #A10462), followed by gateway transfer of KCNJ15 into the lentiviral vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 20 ng of GFP plasmid were transfected into these cells using Lipofectamine 3000 (Invitrogen, L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Expression plasmids for NFKI-1 and IKB-1 were constructed using the pcDNA4TO vector (ThermoFisher Scientific) previously modified to include two HA-epitope units in the 5’ end ...
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bioRxiv - Microbiology 2020Quote: ... All plasmids used in the course of this study were generated using the Seamless Cloning (Invitrogen) technology as previously described (17 ...
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bioRxiv - Genetics 2021Quote: ... with the exception of pCDNA3-RNaseH1 and pCDNA3 plasmids that were transfected using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Three micrograms of the plasmid was then transfected into HeLa cells using Lipofectamine 2000 (Thermo Scientific). Two days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... 4.105 cells were transfected with 3 μg of each gRNA plasmid by Lipofectamine 3000 (Thermo Fisher). Three days post-transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... using 250 ng total plasmid and 0.5 μl of commercial transfection reagent Lipofectamine 2000 (Thermo Fisher). Specifically ...
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bioRxiv - Synthetic Biology 2020Quote: ... using 400 ng total plasmid and 0.6 μl of commercial transfection reagent Lipofectamine 2000 (Thermo Fisher). Specifically ...
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bioRxiv - Biophysics 2019Quote: ... The shorter dsDNA is obtained from a 2.9 kbp pRSET-A plasmid (Thermo Fisher Scientific, V35120) was fabricated in the same way as the PKYBI construct except for the last purification step that is not performed ...
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bioRxiv - Microbiology 2019Quote: ... we incubated 2 µg of plasmid DNA with 125 µL of Opti-MEM (Thermo Fisher Scientific) at room temperature for 5 minutes (min) ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid was transiently transfected into Expi293F cells using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the next day were transfected with 1000 ng GFP-CR plasmids using Lipofectamine LTX (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently purified using the PureLink Quick Plasmid Miniprep Kit (Invitrogen by Life Technologies, Löhne, Germany), and sequenced by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently purified using the PureLink Quick Plasmid Miniprep Kit (Invitrogen by Life Technologies, Löhne, Germany), and sequenced by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Cancer Biology 2021Quote: ... and transfected to HEK293T cells together with packaging plasmids psPAX2 and pMD2G using Lipofectamine 2000 (Invitrogen). Viral supernatants were collected after 48 h ...
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bioRxiv - Developmental Biology 2019Quote: ... E14 ESCs were transfected with 2 ug of the pCAG-Drp1 plasmid using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The pYES-Bgh51wt expression plasmid was constructed by cloning Bgh51wt into pYES3/CT (Invitrogen, Carlsbad, CA). pYES-Bgh51wt was sequenced to ensure the fidelity and transformed into S ...
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bioRxiv - Developmental Biology 2019Quote: ... The plasmids encoding gRNA and hCas9 were co-transfected into mESCs using Lipofectamine 2000 (Gibco, 11668019). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated DNA was cloned into pCR-TOPO plasmids using the TOPO TA cloning kit (ThermoFisher Scientific), and clones were sequenced at the UTSW Sequencing Core.