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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... The gel was imaged and washed 2 x 15 minutes in nuclease-free water then 2 x 15 minutes in 10X UltraPure SSC Buffer (Invitrogen, Carlsbad, CA) with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... the disulfide bond linking the two Fab fragments of F(ab’)2 goat anti-mouse Ig(G+M) was reduced using 2-mercaptoethylamine HCL (Thermo Fisher Scientific) and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 25 mM Tris pH 7.4, 2 mM EDTA, 2 mM EGTA, 100 μM PR-619 (Selleckhem, cat. # 7130) and protease inhibitors cocktail (Thermo Scientific, cat. # 784115,) by vortexing ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... To initiate reaction, 200 µL of Assay Buffer with substrate mixture (30 mM K3PO4, 2 mM MgSO4, 2 mM DTT (Fisher Scientific, AAJ1539706), 0.56 mM TPP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Genomics 2023Quote: ... We combined 2 µg of the plasmid mixture with 2 µl of Lipofectamine™ Stem transfection reagent (Thermo Fisher Scientific, cat# STEM00008) and 100 µl of Opti-MEM I (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections (25 μm thick) from Bregma −2 to +2 (Paxinos and Watson, 2007) were collected onto Superfrost Ultra Plus slides (Thermo Scientific, Germany) air dried at least 2 h at room temperature (RT ...
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bioRxiv - Genomics 2023Quote: ... plugs were melted for 2 minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific, Waltham, MA) for 45 minutes at 43°C ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa (CRM-CCL-2) and U-2 OS (HTB-96) cells obtained from ATCC were cultured in DMEM (Thermo Fisher Scientific, 12800017) supplemented with 10% fetal bovine serum (Lonsera ...
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bioRxiv - Bioengineering 2021Quote: ... N,N-dimethylformamide (DMF) 99% (Acros Organics cat. no. 423640010), 1x phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... in N,N-dimethylformamide (DMF, Thermo Fisher Scientific, D119-20) for 10 min ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of RNase T1 (0.01 U/μL; Ambion), or 2 μL of Shortcut RNase III (0.02 L/μL ...
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bioRxiv - Microbiology 2020Quote: ... 2% Plant RNA Isolation Aid (Ambion, Woodward, TX, USA), 5 mM ascorbic acid ...
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bioRxiv - Biophysics 2021Quote: ... Then 2 equivalents of fluorescein-5-maleimide (Invitrogen, #F150) was added and the mixture was incubated at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-Glutamine (Gibco®, Thermo Fisher Scientific), and a combination of 100 U/ml Penicillin and 100 μg/ml Streptomycin (Gibco® Pen Strep ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-Glutamine (Gibco®, Thermo Fisher Scientific), and a combination of 100 U/ml Penicillin and 100 μg/ml Streptomycin (Gibco® Pen Strep ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Gibco, Thermo Fisher Scientific). After transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Gibco, Thermo Fisher Scientific). After transfection ...
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bioRxiv - Cell Biology 2020Quote: ... or EVOS FL Auto 2 Cell Imaging System (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... + 2 mM Glutamax + 1x non-essential amino acids (Gibco) + 57µM β-Mercaptoethanol (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mg/ml bovine serum albumin (Thermo Fisher, AM2616), RVC (Ribonucleoside Vanadyl Complex ...
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bioRxiv - Microbiology 2021Quote: ... stained with SYTOX Green (2 μM; Life Technologies, USA), and their DNA content was determined using a FACSAria cytometer (Becton Dickinson) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% fetal bovine serum (Gibco-Thermo Fisher Scientific) and antibiotic-antimycotic (Gibco-Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% fetal bovine serum (Gibco-Thermo Fisher Scientific) and antibiotic-antimycotic (Gibco-Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 hours Proteinase K (Thermo Fisher Scietific #AM2548) treatments ...
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bioRxiv - Biophysics 2020Quote: ... a 2% glucose solution is prepared in DMEM (Gibco). Glucose oxidase (0.5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM L-glutamine (Thermo Fisher Scientific, Waltham, MA), 50 U/mL penicillin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Negative control #2 siRNA (Silencer Select, Thermo Fisher Scientific) was used as a negative control.
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bioRxiv - Developmental Biology 2021Quote: ... and 0.2 mM 1-phenyl-2-thiourea (Acros Organics) for 24 h from 1 to 2 dpf.
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bioRxiv - Genomics 2020Quote: ... which contains 2 mg/mL proteinase K (Thermo Fisher), 10 mM Tris (pH = 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μl Superscript II First-Strand Buffer (5x, Invitrogen), 0.1 μl MgCl2 (100 mM ...
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bioRxiv - Cell Biology 2022Quote: ... dissolve in 2 μL dry DMSO (Cat# D12345, Invitrogen). The mixture was incubated for 3 days at 37 °C in the dark for conjugation and purified for 3 rounds using Monarch® PCR & DNA Cleanup Kit (5 μg ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM L-glutamine and 10% FBS (Thermo Fisher). HUVECs (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... and 4U of Turbo DNase (Invitrogen, 2 U/μL) were added to extracts and incubated at 37 °C for 3 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 mM of L-glutamine (Life Technologies, UK), while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and Bis-acrylamide Solution (2% w/v; 10193523; ThermoFisher) and the level of crosslinking in the gel (between ≍ 40 Pa up to supraphysiological values ...
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bioRxiv - Microbiology 2019Quote: ... Cells were fixed with 2% formaldehyde (Thermo Scientific, 28908) on the coverslip for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 2 mM L-glutamine (Gibco, 25030-81), penicillin/streptomycin (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... 2 M thiourea and 4% CHAPS (Thermo Fisher Scientific). Each combined replicate of samples was loaded onto a QIAshredder spin column (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... 2% SDS and 120 μg/ml Proteinase K (Invitrogen)) and incubated 1 hr with continuous shaking (1200 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... After selection with 2 μg/ml puromycin (Life Technologies), the stable clones were picked and validated by Western blot analysis ...