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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and Ctns-/- (n=6) mice were euthanized and the kidneys immediately removed and stored at −80°C in RNA Later (Life Technologies). Tissues were subsequently ground using Precellys 24 (Bertin Technologies ...
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bioRxiv - Bioengineering 2021Quote: Ovaries from 6 to 8 day-old CBA × C57BL/6 (F1) mice were collected and transferred to Leibovitz L-15 media (P/N 11415, Gibco, USA). The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM ...
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bioRxiv - Immunology 2020Quote: ... or empty vector control GFP+ cells (n=6) using TRIzol reagent according to manufacturer instructions (Thermo Fisher Scientific, Waltham, MA, USA). Integrity of total RNA was checked using Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed before switching to the analytical column (BEH C18 ...
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bioRxiv - Genetics 2020Quote: ... Cells were seeded in 6-well plates and transfected with 4 μg/well vectors expressing CasRx-GFP and gRNAs-mCherry (CasRx:gRNA-1:gRNA-2 = 2:1:1, see Supplementary sequences) using Lipofectamine 3000 reagent (Thermo Fisher Scientific). Control group was only transfected with 2 μg/well vectors containing CasRx-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... The air-dried DNA was dissolved in 19 µL of sterile water and treated with Swa1 in two cycles (2×2 FastDigest units, Thermo Scientific), each cycle of 1 hour at 37°C (total volume 25 µl ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS/2% FBS prior to resuspension in PBS/2% FBS supplemented with 0.5 µm Sytox blue (ThermoFisher Scientific S11348) to assess cell viability ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used (8 μg each): F(ab’)2-goat anti-mouse IgG Alexa 647 & F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher). In WGA-Alexa647 labeling experiments ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with or without (Mock) RNase R (2 U/μg) in the presence of Ribolock(2 U/μg) (ThermoFisher Scientific). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... The following dilutions were used for secondary antibodies and cell dyes for staining: goat anti-rabbit Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml, A11070; Molecular Probes), goat anti-mouse Alexa Fluor 488 F(ab’)2 Fragment (2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were decolored with 100% ethanol for 2 minutes and incubated for 2 minutes in xylene before mounting with Permount mounting medium (Fisher scientific). Brain slices were digitized using a Zeiss Axio Scan.Z1 slide scanner (Carl Zeiss ...
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bioRxiv - Neuroscience 2019Quote: ... Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403; Map-2 1:250, Invitrogen PA517646 ...
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bioRxiv - Neuroscience 2019Quote: ... were added into a fresh 2 ml microcentrifuge tube and washed with 1ml of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed for 6 min before switching the precolumn in line with the analytical column (BEH C18 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with KIF-encoding plasmid and empty vector pcDNA3.1 (+) (1 µg total DNA) using 2 µl Lipofectamine 3000 Reagent and 2 µl P3000 (Thermo Fisher Scientific). hTERT-RPE1 cells stably expressing SMO-tRFP were transfected a day after seeding and IMCD3 cells expressing mCherry-Arl13b were reverse transfected at the time of seeding ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were mounted onto a Warner chamber and the cells were loaded with 2 µM Fura-2/AM (Molecular Probes) in Ca2+ Imaging Buffer (Ca2+ IB;10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were loaded in loading buffer (2% MeCN, 0.05% trifluoroacetic acid) and bound to an Acclaim Pepmap 100 µm × 2 cm trap (Thermo Fisher Scientific), and washed for 10 min to waste ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2019Quote: ... Muscles were extensively washed with PBS for at least 2 hours and then incubated for additional 2 hours with appropriate secondary antibodies (Life Technologies) supplemented with fluorescently labelled α-bungarotoxin (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were plated at a density of 58,000 cells/cm2 on PDL-coated 12 mm coverslips and maintained in neurobasal media supplemented with 2% B27 and 2 mM Glutamax (Life Technologies) at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Japan), 50 μg/mL streptomycin (Meiji Seika Pharma, Japan) and 0.1 mM 2-mercaptoethanol (2-ME; 21985-023, Thermo Fisher Scientific). For the maintenance of PKA-ESCs ...
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bioRxiv - Genetics 2021Quote: ... and 1.5 µg of each of the sgRNAs plasmids (PX330-Nanog-sgRNA1/2 and PX330-Esrrb-sgRNA1/2) using 16.5 µl of lipofectamine 3000 and 22 µl of P3000 (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... cell pellet of 1 x106 – 2 x106 PBMCs was resuspended in 2 ml erythroblast cell expansion media containing basal blood media Stempo34 SFM (Thermo Fisher) supplemented with 1x Stempo34 nutrient supplement (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... were cultured to a density between 2*105 and 2*106 cells per ml in complete media [RPMI 1640 with HEPES and GlutaMAX (Gibco #72400120), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Filters were first cut into 2 mm by 2 mm pieces using scissors sterilized and treated with RNase AWAY surface decontaminant (Thermo Scientific). Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... were added to Au-flat 2/2 (2 µm hole, 2 µm space) cryo-electron microscopy grid (Protochips, Morrisville, NC, USA) using a Vitrobot Mk IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Neuroscience 2023Quote: ... The following day cells were rinsed with PBS and incubated for 2 hours with the appropriate Alexa Fluor-conjugated secondary antibodies (2 μg/mL, Thermo Fisher) and DAPI (1 μg/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
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bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...