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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (goat-anti-mouse Alexa Fluor 488 and goat-anti-rat Alexa Fluor 555 (both 1:500, Invitrogen) were incubated for 1.5h in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... stained with cell surface antibodies at 1:1200 (anti-CD8: PerCP-Cyanine5.5, eBioscience, # 45-0081-82, or Biolegend, #100734, anti-CD44: APC, Invitrogen, #17-0441-82 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with α-bungarotoxin and goat anti-rabbit and anti-mouse secondary antibodies conjugated to Alexa Fluor-488 (Invitrogen), and washed again ...
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bioRxiv - Neuroscience 2023Quote: ... and either AlexaFluor-488/647-conjugated goat anti-mouse or anti-rabbit IgGs (1:400) and Hoechst (1:10,000; Invitrogen). For tissue expansion ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were-donkey anti-rabbit or donkey anti-mouse tagged with either Alexaflour488 or Alexafluor568 (1:1000; Invitrogen) or goat anti-rat Alexaflour350 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated anti-mouse IgG (Cat# PA1-28568) and anti-hamster IgA (sab3003a) were obtained from Invitrogen (Waltham, MA) and Brookwood Biomedical (Jemison ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 633-conjugated secondary antibodies (Goat anti-Mouse, Invitrogen A21052 or Goat anti-Rabbit, Invitrogen A21071 as appropriate) were used at a 1:15000 dilution.
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bioRxiv - Genetics 2023Quote: ... The secondary antibodies used in this study were anti-rabbit Alexa 488 and anti-mouse Alexa 568 (Life Technologies). Samples were then washed 5 times in PBS-T for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with Alexa 488 anti-rabbit IgG (green) and Alexa 594 anti-mouse IgG (red) (Molecular Probes) in PBS containing 3% BSA for 30 min at room temperature ...
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Dose-dependent effects of histone methyltransferase NSD2 on site-specific double-strand break repairbioRxiv - Cell Biology 2023Quote: ... 2 μg of anti-NSD2 antibody (ab75359) and 20 μl of Dynabeads M-280 sheep anti-mouse IgG (Invitrogen) were mixed in immunoprecipitation dilution buffer and incubated for 6 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039; anti-mouse-Alexa-Fluor-647, Invitrogen A21235 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with secondary antibodies (Goat anti-mouse, Invitrogen A32727; Goat anti-rabbit, Invitrogen A32731, 1:1000 dilution) for an hour at room temperature ...
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bioRxiv - Physiology 2023Quote: ... incubated in appropriate secondary antibodies (Anti-rabbit IgG, 7074, Cell Signaling Technology; or anti-mouse IgG, 31450, Thermo Scientific), and washed in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... then probed by Western blotting using anti-GPR37L1 (Alomone Labs, Rabbit, 1:1000, Cat No.AGR-050) or anti-Kir4.1 (Thermo scientific, Guinea pig ...
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bioRxiv - Neuroscience 2023Quote: ... we used Primocin (100 μg/ml final concentration) and anti-mycotic/anti-biotic (1X final concentration, Gibco Catalogue #15240062). For PMA-mediated activation ...
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bioRxiv - Neuroscience 2023Quote: ... freshly isolated FAPs were cultured at 37L°C in alpha-MEM (Hyclone) supplemented with Antibiotic-Antimycotic (anti-anti, Gibco) and 20% FBS (Hyclone) ...
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bioRxiv - Biochemistry 2023Quote: ... before being incubated for 20 min at 4°C with anti-V5-FITC or anti-V5-PE (ThermoFisher Scientific) and anti-CD8-APC (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with Alexa-Fluor goat anti-mouse 633 or goat anti-rabbit Alexa-Fluor 647 (Life Technologies) as secondary antibodies for at least 1 hour ...
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bioRxiv - Physiology 2024Quote: ... Alexa Fluor 594 donkey anti-rabbit (1:500) and Alexa Fluor 488 donkey anti-mouse (1: 500; Molecular Probes, Cat# A-21207 ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... and Flag tagged (NLLRR) proteins were detected with 1/5,000 dilution of the anti-Myc and anti-Flag (cat# MA1-91878, Thermofisher) primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The primary and secondary antibodies were diluted in PBST plus 1% BSA by 1:500-1:2,000 for primary antibodies (ab10799 for histone H3 and ab4729 for H3K27ac) and 1:5,000 for the secondary antibodies (Alexa-488 anti-mouse and Alexa546 anti-rabbit from Invitrogen). The primary antibody was incubated overnight at 4 °C in a moisture chamber ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies (Goat anti-Mouse IgG Alexa Fluor 488, Invitrogen; Goat anti-Rabbit IgG Alexa Fluor 647, Invitrogen) were diluted 1:500 in blocking solution and incubated for 60 minutes at room temperature in the dark under gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit and Alexa fluor 550-conjugated goat anti-mouse were from Thermo Fisher Scientific (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... The appropriate secondary antibodies (anti-rabbit DyLight800 [SA5-35571; ThermoFisher Scientific], or goat anti-mouse DyLight680 [35568; ThermoFisher Scientific]) were used and images were acquired with a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Pathology 2024Quote: ... Cochleae were stained with anti-prestin antisera (44) followed by goat anti-rabbit Alexa Fluor 488 secondary antibody (ThermoFisher), together with Alexa Fluor 568 phalloidin and Hoechst 33342 (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labeled with AF532 donkey anti-mouse and AF488 donkey anti-rabbit secondary antibodies (both ThermoFisher, 1:1000) and slides were mounted in ProLong Diamond Antifade with DAPI.
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were Alexa Fluor 488 or 546-conjugated goat anti-rabbit IgG and goat anti-mouse IgG (Invitrogen)(1:500) ...
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bioRxiv - Immunology 2024Quote: ... primary human CD4 T cells were activated with anti-CD3+ anti-CD28 Dynabeads (1:1) (Thermofisher scientific, cat # 11161D) + IL-2 overnight and then 1 million aliquots of cells (triplicates ...
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bioRxiv - Immunology 2024Quote: ... The secondary antibodies were Alexa Fluor goat anti-mouse IgG2a or IgG2b or goat anti-rabbit IgG (Life Technologies) and goat anti-mouse IgG1 (Southern Biotech) ...
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bioRxiv - Neuroscience 2024Quote: ... then with anti-rabbit IgG-Alexa Fluor 488 or anti-rat IgG-Alexa Fluor 594 (1:1000, Invitrogen, USA) for 2 h at room temperature (RT) ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... RNA-protein complexes were immunoprecipitated using 5 μg antibody (either anti-ZBTB48, or anti-GFP antibody (Life Technologies G10362), or anti-FLAG ...
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bioRxiv - Cell Biology 2020Quote: ... One µg of total RNA was retrotranscribed into complementary cDNA using an RT-PCR kit (High capacity cDNA rt Kit, Applied Biosystems). Quantitative PCRs were carried out in triplicate of each sample using 40 ng of cDNA per well ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The fat body enriched tissues were transferred into Eppendorf with 200µL of homogenization buffer from the RNA isolation kit (MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher). The tissues were homogenized and stored at -80°C until RNA extraction ...
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bioRxiv - Developmental Biology 2020Quote: ... The libraries were profiled with High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... pregnant mice were injected intraperitoneally with 200 mg/kg body weight of 5-Ethynyl-2’-deoxyuridine (contained in the Click iT EdU Imaging Kit containing Alexa Fluor 594 as the Alexa Fluor azide (Kit #C10338; Thermo Fisher) 1 h before embryo collection at E9.5 ...
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bioRxiv - Physiology 2022Quote: RNA was extracted from each hemi-embryo following the manufacturer’s instructions using PicoPure RNA isolation kit (PicoPure RNA isolation kit, Applied Biosystems, France), which included a DNAse treatment ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated using the RNeasy Micro kit and cDNA synthesized a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR amplified products were run on 1.5% agarose gel and the specific amplified band was eluted using gel elution kit (GeneJET Gel Extraction Kit; Thermo Scientific, USA). Sequence determination was performed for both the strands of the DNA by Chromous Biotech ...
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bioRxiv - Genomics 2019Quote: ... The libraries were profiled with High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474) and measured with Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851) prior to pooling and sequencing using the Illumina NextSeq 500 platform using custom ReadOne primer (IDT ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted using the GeneJET Genomic DNA Purification Kit while plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... PCR products together with a non-template control were checked on 1% agarose gels and then cleaned with the Qiagen PCR Purification Kit and sequenced using an ABI 3130xL sequencer and the BigDye Cycle Sequencing Kit (Applied Biosystems). In total we found four mtDNA haplotypes ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was isolated using E.Z.N.A.® MicroElute Total RNA Kit (Omega Bio-tekkit) and transcribed into complementary DNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time PCR reactions were carried out using 1x GoTaq™ qPCR Master Mix (Promega ...
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bioRxiv - Evolutionary Biology 2019Quote: ... purified fosmid DNA was first fragmented into approximately 1.5 kb fragments using a Nebulizer kit supplied in the TOPO shotgun Subcloning kit (Invitrogen, Carlsbad, CA), and then sub-cloned into pCR4Blunt-TOPO vectors ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted later on from each hemi-embryo using PicoPure RNA isolation kit (PicoPure RNA isolation kit, Applied Biosystems, France), which included a DNAse treatment ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in PBS and semEV concentrations were estimated by BSA protein kit (Thermo Fisher, Pierce BCA Protein Assay Kit) and adjusted to 2mg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Library quality and quantification was assessed on the Agilent Fragment Analyzer using a High Sensitivity NGS Kit (DNF-474) and a Qubit 4 RNA BR kit (Thermo Fisher). Samples were then normalized to 4nM and pooled in equimolar amounts ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were sequenced using BigDye Terminator v3.1 cycle sequencing kit and cleaned with BigDye XTerminator Purification kit (Applied Biosystems, USA). Products were analysed on a 3130xl Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: Subcellular protein fractionation and isolation of chromatin fractions was performed using the Subcellular Protein Fractionation Kit for Cultured Cells kit from Thermo Fisher, following the instructions of the manufacturer ...
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bioRxiv - Microbiology 2022Quote: Our metabolic click-labelling was based on the Click-iT HPG Alexa Fluor protein Synthesis Assay Kit (Click-iT HPG Alexa Fluor Protein Synthesis Assay Kit, ThermoFisher C10428) strategy ...