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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
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bioRxiv - Genomics 2023Quote: ... and 6 mg magnetic beads (per sample) were conjugated overnight using the Dynabeads Antibody Coupling Kit (Invitrogen, 14311D). The next day ...
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bioRxiv - Neuroscience 2023Quote: Eight-micron sections of L4-6 DRG were blocked for two hours in Superblock (ThermoFisher; Grand Island, NY), 1.5% Normal Donkey Serum ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitated DNA was analyzed by qPCR on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with Power SYBR Green PCR Master Mix and calculated as % of input ...
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bioRxiv - Cancer Biology 2022Quote: ... Target genes were amplified using PowerUp SYBR Green Master Mix and a QuantStudio 6 thermocycler (Thermo Fisher Scientific). Gene expression was calculated using the ΔΔCt method for candidate genes ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1, Thermo Fisher Scientific, A1113903) and hygromycin B (0.3 mg mL-1 ...
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bioRxiv - Cell Biology 2023Quote: ... Slide Book Software 6.0 (Intelligent Imaging Innovations) and QuantStudio 6 Real-Time PCR Software (Applied Biosystem, Thermo Fisher). *P<0.05 ...
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bioRxiv - Genomics 2023Quote: ... was added and 10μL of mixture was loaded into 6% DNA Retardation Gels (cat. EC6365BOX, Thermo Fisher Scientific). Gels were run at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The real-time qPCR reactions were performed using the QuantStudio 6 Flex real-time PCR system (Applied Biosystems), of which the parameters were set to 50°C for 2 min ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Cell Biology 2023Quote: Transient transfections in HeLa and Rme-6-ve cells were performed using either Polyfect or Lipofectamine3000 (ThermoFisher, Ltd), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cells cultured in 6 well plates were washed with PBS and fixed with 4% Paraformaldehyde (11400580, Fisher Scientific) in PBS for 5min at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... All RT-qPCR experiments were performed with a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... All samples were run in triplicate on a Quant Studio 6 Flex machine (Applied Biosystems, Waltham, MA, USA) and analyzed using QuantStudio Design and Analysis Software (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments.
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bioRxiv - Microbiology 2023Quote: ... saquinavir 40 μM or 20 μM) for 6 h and lysed in 0.15% w/v saponin (ThermoFisher Scientific) for 10 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (Quant Studio 6 Flex; Applied Biosystems) with SYBR green PCR master mix (Life Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Cells were then co-transfected with the two plasmids at a molar ratio of 6:1 (2.5 μg of homology vector, ∼0.7 μg of sgRNA vector) using Lipofectamine2000 (ThermoFisher). The next day ...
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bioRxiv - Immunology 2023Quote: ... where indicated along with 6 μg pCL-Eco plasmid DNA using 60 μl Lipofectamine 2000 in OptiMEM (Invitrogen) for 8 h in antibiotic-free medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Using qRT-PCR (QuantStudio 6, ThemoFisher Scientific) and the SYBR Green Real-time PCR master mix (Thermo Scientific), the expression levels of certain genes were determined.
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bioRxiv - Cancer Biology 2023Quote: A total of 6 × 106 NK cells were stained with CellTracker™ Deep Red Dye (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 30 min at 30 °C and separated on 6% polyacrylamide DNA retardation gels (Invitrogen). Gels were dried and analyzed by phosphorimaging (Fuji).
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bioRxiv - Genomics 2023Quote: ... hippocampus tissue from three adult C57BL/6 mice was pooled and RNA extracted (Trizol reagent, Invitrogen Cat# 15596026). RNA was used as input for a FirstChoice RLM-RACE Kit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR reactions were run in a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher, Cat. No. 4485691) and Ct values were obtained using built-in software ...
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bioRxiv - Neuroscience 2024Quote: ... 6 ml of stock solution was added to 294 mL of 0.1% acetic acid (Cat# UN2789, Fisher Scientific) in ddH2O and used within 10 min of preparation ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 6% foetal bovine serum (FBS) and Penicillin-Streptomycin (10,000 U/mL) all purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... B-7-6 was produced in our own laboratory and was directly conjugated to DyLight (Pierce/Thermo Scientific) fluorochromes ...
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bioRxiv - Biochemistry 2024Quote: The GPR65-Gs pH 6 complex was concentrated to 11 mg/mL supplemented with 0.05% CHAPS (Thermo Fisher) and 3 µL was applied onto a glow-discharged 300 mesh 1.2/1.3 gold grid covered in a holey gold film (UltrAufoil) ...
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bioRxiv - Genetics 2024Quote: ... was used and quantitative RT-PCR was performed on QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific). Gapdh was used as the internal control ...
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bioRxiv - Neuroscience 2024Quote: After a wash in Carnoy’s solution (6 ethanol: 3 chloroform: 1 acetic acid; all from Thermo Fisher, USA), used to removed most of the lipids from the section [45] ...
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bioRxiv - Biochemistry 2024Quote: The GPR4-Gs pH 6 complex was concentrated to 14 mg/mL supplemented with 0.05% CHAPS (Thermo Fisher) and 3 µL was applied onto a glow-discharged 300 mesh 1.2/1.3 gold grid covered in a holey gold film (UltrAufoil) ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... hTSC were cultured in pre-coated 6-well plates (1 µg/ml Col IV) with 2 ml of EVT differentiation medium (EVTM: DMEM/F12, 0.1 mM 2-mercaptoethanol (Gibco 31350)) ...
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bioRxiv - Immunology 2022Quote: ... ∼50% of total lung was placed in a bead homogenizer tube with 1 mL of PBS with 2% FBS and 2% antibiotics/antimycotics (Gibco) and stored at -80°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were plated in 24-well plates and the plasmids (psi-check-2-WT, psi-check-2-mut) were transfected using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...
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bioRxiv - Bioengineering 2021Quote: ... Silica gel was prepared by adding 2 g silica particles to 40 ml aqueous NaOH (0.01N; CAS 1310-73-2; Fisher Scientific) solution and magnetically stirring the mixture at a speed of 1000 rpm for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The gland/crypt-containing supernatant was washed by centrifugation at 400 x g for 2 minutes and the glands/crypts re-suspended in 1-2 ml advanced DMEM/F12 (12634-010; Gibco) containing 1X B27 supplement minus vitamin A (12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL (200 pmol) each of gRNA and tracRNA were combined with 2 μL of 5X annealing buffer (ThermoFisher 100061876) and 4 μL nuclease-free dH2O ...
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bioRxiv - Microbiology 2021Quote: ... coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen) and incubated for additional 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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bioRxiv - Bioengineering 2022Quote: ... Porcine DFAT adipocytes were stained for lipids via a 15-30 minute incubation at 37°C in lipid accumulation media (sans Intralipid) supplemented with 2 μM BODIPY 493/503 and 2 μg/ml Hoescht 33342 (H3570; Invitrogen). DFAT cells were then fixed in 4% PFA at RT for 10-15 minutes ...
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bioRxiv - Microbiology 2022Quote: The monoclonal antibodies SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) and SARS-CoV-1/SARS-CoV-2 Nucleocapsid (6H3) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...