Labshake search
Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
-
bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Samples of the visceral yolk sac (n=6/group) were immersed in RNA Later solution (Invitrogen, MA, USA) and frozen at −20°C until further processing ...
-
bioRxiv - Systems Biology 2021Quote: ... splenocytes were obtained from naïve C57BL/6 mice and stained with CellTrace Pacific Blue Cell Proliferation Kit (ThermoFisher). Stained splenocytes (2.5 × 105 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and size selected for 130-200 nt fragments on a pre-casted 6% polyacrylamide Novex TBE gel (Invitrogen). Gel extraction was done using Gel breaker tubes (IST Engineering Inc ...
-
bioRxiv - Cancer Biology 2020Quote: ... mitochondria were purified from 4-6 × 107 cells using mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR reactions were performed in triplicate using QuantStudio 6 Flex Real-Time fast PCR System (Applied Biosystems) and 2-ΔΔCt values were used to calculate the relative expression level of the target genes compared to controls ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... PDMS coverslips were manually added to each well of 96/6 well plates (ThermoFisher Scientific, Waltham, MA USA) and each plate was subsequently sterilized ...
-
bioRxiv - Developmental Biology 2019Quote: ... 6 mM β-mercaptoethanol and 0.5% NP-40) supplemented with 75 units/ml RNAse inhibitor (Thermo Fisher Scientific) and 1X mammalian protease inhibitor cocktail (Roche ...
-
bioRxiv - Systems Biology 2020Quote: ... diluted to 100mM HEPES and 4M GnHCl and pH adjusted pH6.5-7.5) prior to 6-plex TMT labelling as per manufacturer’s instructions (Sixplex TMT, Cat# 90061, ThermoFisher Scientific). Equal ratios of TMT-labelled samples were pooled and methanol/chloroform precipitated ...
-
bioRxiv - Bioengineering 2021Quote: The 6 most concentrated fractions were concentrated and desalted with 6mL 3MWCO protein concentrator columns (Pierce, Thermo Fisher) at 4C 4000xg 4 hrs and stored at 4C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with 6 μg plasmid DNA per 106 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V ...
-
bioRxiv - Developmental Biology 2019Quote: ... day 4- and day 6-control and mutant ESC-derived neurospheres were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...
-
bioRxiv - Molecular Biology 2020Quote: mESCs were dissociated with Accutase™ and seeded in Nunclon Sphera 6-well plates (Thermo Fisher Scientific, # 174932) at a cell density of 11,000 cells / well in Serum + LIF medium ...
-
bioRxiv - Cell Biology 2019Quote: ... RFL-6 cell were purchased from the American Type Culture Collection (ATCC) Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS ...
-
bioRxiv - Biochemistry 2019Quote: ... or mismatch plasmids (1.5 μg per well in 6-well plate) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.
-
bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
-
bioRxiv - Genetics 2021Quote: ... The relative mtDNA copy number was assessed using QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific). The mtDNA and the nuclear-encoded housekeeping gene 18S were amplified using primer sequences ...
-
bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
-
bioRxiv - Neuroscience 2019Quote: ... the medium in the 6 well plates was replaced with pure OMEM without phenol (#11058-21, ThermoFisher Scientific) by adding 1ml per well ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
-
bioRxiv - Physiology 2021Quote: ... 6-10 microliters of a mixture of green fluorescent particles (Thermo Scientific, 1.05 g/cm3, fluorescence 589 nm) were injected using a 2.5 μm Hamilton glass syringe with a pulled borosilicate capillary tube (Fig.2A) ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and cultured for 6 days in maintenance medium comprising Dulbecco’s minimum essential media DMEM/F12 (77.5 % v/v, Gibco), Knockout Serum Replacement (20 % v/v ...
-
bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell pellets were then resuspended and plated into one well of a 6 well plate (Fisher Scientific 1483211). Cells were left to sit for 5 days at 37°C and 5% CO2 after initial plating and were only fed once after three days by adding an additional volume of FCM ...
-
bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
-
bioRxiv - Biochemistry 2021Quote: ... quantitative PCR was performed using a Quant-Studio 6 Flex Real-Time PCR instrument (Thermo Scientific, Waltham, MA). For sample preparation ...
-
bioRxiv - Cell Biology 2020Quote: ... 1.8 mM CaCl2, 6 mM NaHCO3, 5.5 mM D-glucose, 25 mM HEPES, pH 7.4 supplemented with Gibco MEM Amino Acids and MEM Non-Essential Amino Acids solution ...
-
bioRxiv - Immunology 2020Quote: ... For poly(I:C) stimulation cells were transfected in 6-well plates with 10 μg polyI:C using Lipofectamine 2000 (Invitrogen) for 2 h before imaging ...
-
bioRxiv - Immunology 2020Quote: ... or SYBR Green chemistry and reactions were run on a RT-PCR system (QuantiStudio 6 Flex, Applied Biosystems). Gene expression is displayed as fold increase over antibiotic-pre-treated ...
-
bioRxiv - Cell Biology 2021Quote: ... and selected antibiotics according to the respective expression constructs: blasticidin S (6 μg mL−1, Thermo Fisher Scientific), puromycin (0.5 μg mL−1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were seeded in 6-well plates and transfected the following day using lipofectamine 3000 (Thermo Fisher Scientific). 20 mM oleic acid complexed with 2.5 mM BSA were added to cells 16 h post transfection ...
-
bioRxiv - Bioengineering 2022Quote: CD19+ NALM-6 cells (B cell acute lymphoblastic leukemia) were stained with Cell Trace Violet (Thermo Fisher Scientific) and primary human CD8+ T cells were stained with either Cell Trace Yellow (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... rested for 6 hours and stimulated overnight in 1 ml of complete medium (RPMI 1640 with Glutamax, Gibco) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... 70% confluent HEK293T cells in 6 well plates were transfected in Opti-MEM reduced serum medium (Thermo Fisher) with 250 ng of 8 plasmids ...
-
bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
-
bioRxiv - Microbiology 2022Quote: ... Each of the three biological replicates were quantified in technical duplicates using Quantstudio 6 Flex (Applied Biosystems #4485694) incubating at 50°C for 10 min ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔFZD1-10 or ΔLRP5/6 cells were transiently transfected in suspension using Lipofectamine 2000 (Thermo Fisher Scientific 11668). An approximately 80-90% confluent T-75 flask was re-suspended in 20 ml yielding a suspension with 4-5×105 of cells ml-1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5μg Cas9/gRNA containing plasmid and 1.5μg repair template were co-transfected into 6-well dishes using Lipofectamine 3000 (Invitrogen) with a 3:1 transfection reagent to DNA ratio for 48 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were mixed and dispensed at 120k cells/per well (6 well size) coated with 1x Geltrex (ThermoFisher). Cells were incubated at 37 °C incubator overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed 6-8 days after the last CDSD session and RNA was extracted with TriReagent (Ambion). Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre ...
-
bioRxiv - Cell Biology 2022Quote: ... were used for qPCR in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol for 40 cycles ...
-
bioRxiv - Physiology 2022Quote: ... RT-qPCR was performed using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham MA) with a 384-well platform ...
-
bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
-
bioRxiv - Genomics 2022Quote: ... 10-20×10^6 adherent cells were first removed from the plate using StemPro Accutase (ThermoFisher, A11105-01) or TrypLE Express (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were plated on 12×12 mm coverslips in 6-well plates (ThermoFisher Scientific, cat# 140675). The next day cells were pre-treated with 2 mM Thymidine (Sigma-Aldrich ...