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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for Recombinant Human UBE2Z Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were centrifuged at 1400 rpm for 4 minutes and incubated with Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (ThermoFisher, MA5-14520) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 33 µl of this buffer was added to the beads together with 10 µl Recombinant Proteinase K Solution (20 mg/ml, Thermo Fisher, AM2548) and 3 µl RiboLock RNase inhibitor (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Cancer Biology 2021Quote: ... respectively coupled with protein G Dynabeads (Invitrogen) with nuclear extract derived from MCF-7 cells for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Dynabeads Protein A or G (Life Technologies), 25 µl per IP ...
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bioRxiv - Bioengineering 2021Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Biophysics 2020Quote: ... B-PER Bacterial Protein Extraction Reagent (ThermoFisher) was used to lyse the cells in the presence of protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: ... or DynabeadsTM protein G beads (#10004D, Invitrogen) were added into each sample and incubated at 4 °C for 1-2 hours on the next day ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein G Dynabeads (Thermo Fisher Scientific, #10003D), protein G beads (EMD Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Sypro Ruby Protein Blot Stain (Molecular Probes) was used to quantify protein concentration in each sample ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabead Protein G beads (ThermoFisher, Waltham MA) were added to a concentration of 30 µL/mL and rotated for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: 100 µl of Dynabeads Protein A (ThermoFisher) were prepared for immunoprecipitation (IP ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations was determined by Thermo Fisher BCA assay kit and then 50μg of protein was loaded into the pre-coated VEGFA ELISA kit (R&D systems) ...
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bioRxiv - Genetics 2021Quote: ... 30 pmol TrueCut Cas9 Protein (Invitrogen #A36498) and 15 pmol gRNA were combined for a ratio of 2:1 ...
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bioRxiv - Genetics 2021Quote: ... Dynabead protein A (50ul; Thermo Fisher 10001D) and protein G (50ul ...
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bioRxiv - Microbiology 2019Quote: ... bound to protein A magnetic beads (Invitrogen). After three washes with 1X IP buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... were conjugated with Dynabeads Protein G (Thermofisher) magnetic beads ...
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bioRxiv - Developmental Biology 2020Quote: ... 20 μl of protein G Dynabeads (Invitrogen) per aliquot were washed twice with ChIP dilution buffer and resuspended in 50 μl/aliquot of the same solution ...
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bioRxiv - Developmental Biology 2021Quote: ... Dynabeads Protein G (10003D, Thermo Fisher Scientific) and rat anti-HA antibody (3F10 ...
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bioRxiv - Microbiology 2022Quote: ... A micro-BCA protein assay (Thermo Fisher) was performed in plates coated with Ag and washed to demonstrate that all peptides bound to the well surfaces in the plates (data not shown) ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μL of Protein G Dynabeads (Invitrogen) were pre-incubated with 2.4 μg of either CaMKIIα (Leonard et al. ...
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bioRxiv - Neuroscience 2021Quote: ... protein-free CD hybridoma medium (Gibco 11279023). S9.6 antibodies were purified according to standard procedures (Stork et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 70ul of Protein G Dynabeads (Life Technologies) was blocked with 0.5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to nitrocellulose membrane (Invitrogen) and probed with the indicated antibodies.
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bioRxiv - Molecular Biology 2021Quote: ... protein adsorbing 384-well microtiter plates (Nunc® MaxisorpTM ...
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bioRxiv - Molecular Biology 2021Quote: ... 50µl Protein A Dynabeads (Thermo Fisher Scientific) were washed with lysis buffer 3 times for 3min each ...
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bioRxiv - Molecular Biology 2021Quote: ... 50µl protein G magnetic beads (10004D, Invitrogen) were used to pull-down the immune complexes at 4°C for 2-3h ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl Protein G Dynabeads (Invitrogen, 10003D) per sample were washed 3 times with wash buffer (1:1 dilution of high salt ...
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bioRxiv - Molecular Biology 2019Quote: ... and 75 μl protein G Dynabeads (Invitrogen), were washed 3 times in IP buffer before adding to the sample and incubating for 2 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μL Protein A beads (Thermo Fisher) were used per IP and transferred to a 2 mL Eppendorf tube on a magnetic stand ...
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bioRxiv - Developmental Biology 2019Quote: ... Molecular weight markers (BenchMark Protein Ladder, Invitrogen) were also run ...
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bioRxiv - Neuroscience 2019Quote: ... A BCA protein assay kit (ThermoFisher Scientific) was used to determine protein concentration ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 ul of Dynabeads protein G (ThermoFisher) were bound to FLAG antibody (F1804 ...
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bioRxiv - Cell Biology 2019Quote: ... antibody and protein A beads (Thermo Fisher) at 4 °C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... or Pierce BCA protein assay (Thermo Scientific).
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bioRxiv - Genomics 2020Quote: ... Protein G Dynabeads (Invitrogen; 30 μl/reaction) were equilibrated in TBSTBp (TBS with 1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A/G Dynabeads (Thermo Fisher Scientific) were incubated for at least 6 hours at 4°C with an antibody against AR (9 μg ...
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bioRxiv - Genomics 2019Quote: ... and 25μL Protein G dynabeads (Life Technologies) in modified RIPA buffer over night at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... antibodies bound to protein A Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 µl of Dynabeads Protein G (Invitrogen) was then added and incubated for an additional 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... 50 µl of Dynabeads Protein G (Invitrogen) were added to each ChIP reaction and incubated for 4 hours at 4℃ ...
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bioRxiv - Biochemistry 2019Quote: ... SeeBlue pre-stained protein standard (Invitrogen # LC5925) was used to determine the molecular weight ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 µL Protein G Dynabeads (Thermo Fisher) per sample were washed three times in MeRIP buffer (150 mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... or protein A-Sepharose beads (101041, Invitrogen) and anti-phospho-Threonine-Proline antibody (1:5000 ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated to protein G magnetic beads (Thermofisher) in the presence of 200unit of RNaseOut per milliliter of lysates ...