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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Recombinant Human UBE2Z Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). The concentration of samples was adjusted by dilution with RIPA buffer ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The EV protein concentration was measured by using a Micro BCA Protein Assay Kit (23235, Thermo Fisher Scientific). The collection method ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations in cell lysates were then normalized with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay (Pierce 660 nm Protein Assay Reagent (ThermoFisher Scientific), complemented with Ionic Detergent Compatibility Reagent (IDCR ...
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bioRxiv - Cell Biology 2024Quote: ... protein concentration was determined using PierceTM BCA Protein Assay Kit (Cat. No. 23225, Thermo Scientific, Waltham, MA, USA) and equal protein amounts were mixed with SDS-containing Laemmli buffer and loaded onto SDS-polyacrylamide gels ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the protein concentration was determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were cleared using centrifugation and total protein concentration was measured with BCA protein assay (Thermo Scientific 23225). 30-50 µg total protein was separated on 4-12% Bis-Tris protein gels (Invitrogen NuPAGE ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of the samples were determined using a Thermo BCA protein assay kit (Thermo Fisher Scientific) and adjusted to 1 mg/ml for SDS‒PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were stored at −80°C for later protein extraction (TPER Tissue Protein Extraction Reagent (Thermo Scientific: 78510) + HALT (1x)) ...
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bioRxiv - Developmental Biology 2024Quote: ... The concentration of protein was determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher, A53225). Aconitase was measured according to the Aconitase Activity Assay Kit.
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bioRxiv - Physiology 2024Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 µg of total protein ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein fractions were quantified using an 8-point Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, PI23225), and 20 µg of protein per sample diluted to 35µL using NuPAGE LDS sample buffer (1x final conc. ...
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bioRxiv - Cell Biology 2024Quote: ... Results were normalized to total protein concentration in the cell lysate (Pierce BCA protein assay, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration in this suspension was again determined using a PierceTM BCA Protein Assay (Thermo Fisher Scientific) and adjusted to 2 mg/mL using PBS ...
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bioRxiv - Neuroscience 2024Quote: Protein synthesis was assessed using the Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration was assayed in the supernatants using the Pierce BCA Protein Assay Kit (Fisher Scientific, 23225). GCase activity measurements were performed as previously described17 ...
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bioRxiv - Bioengineering 2024Quote: ... The protein concentration of each sample was determined by BCA assay (Thermo Scientific, Pierce BCA Protein Assay Kit) relative to a bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration was determined in the samples using the Pierce BCA Protein Kit (Thermo Fisher Scientific Inc). The solid protein was denatured at 95°C for 5 min in NuPAGE LDS Sample Buffer (4X ...
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bioRxiv - Bioengineering 2024Quote: ... Total protein concentration was quantified by Micro BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific). Samples and migration standards were prepared under reducing conditions and incubated for 10 min at 37 °C prior to loading ...
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bioRxiv - Immunology 2024Quote: ... 0.6 µg gRNA was mixed with 2 µg Cas9 protein (TrueCut™ Cas9 Protein v2, Thermo Fisher Scientific) in 5 µl E8 medium supplemented with 10 μM Y27632 ...
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bioRxiv - Immunology 2024Quote: ... Prior to incubation completion Pierce Protein A/G Agarose Beads (ThermoFisher Scientific, 30 μL per 1 mg protein) were washed with ice-cold PBS (1 mL ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of lysates was tested by the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). For the reducing gel ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE on NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels (Invitrogen) in MOPS running buffer ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... protein concentrations of sheared chromatin were quantified with the Pierce™ BCA Protein Assay Kit (Thermo Scientific, #23227) following manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were then separated by SDS-PAGE electrophoresis with a 4-12% Bis-Tris protein gel (Fisher Scientific) in 1x MOPS SDS Running Buffer (Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in each biological replicate was measured by Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was kept it in ice and protein concentration were measured with QUBIT protein kit (#Q33211, ThermoFisher).
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bioRxiv - Cancer Biology 2024Quote: ... and estimated the protein concentrations using Pierce BCA Protein Assay Kit as per the manufacturer’s protocol (ThermoFisher Scientific). Around 10 µg of proteins were allowed to resolve in 8% SDS-PAGE separating gel ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus carrying hXkr8-TH-mGFP or hBSGΔ cDNA was obtained with the Bac-to-Bac Baculovirus Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... the GST-Tagged proteins were eluted with 15 mM GSH (Bio-basic) and cleaved in solution using purified recombinant His-Tagged TEV protease (Thermo Fisher Scientific). Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Biochemistry 2022Quote: ... Rhodopsin expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Bioengineering 2021Quote: ... Qiagen miniprep Kit`for recombinant plasmid isolation and purification and SDS-NuPAGE precast gels for molecular characterization of expressed enzyme were obtained from Invitrogen (Carlsbad, CA). Polygalacturonic acid (PGA ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with a polyclonal antibody raised in rabbit against purified recombinant BT1636 (BT1636Ab, Cocalico Biologicals) and detected with an Alexa Fluor® 488-conjugated goat anti-rabbit IgG secondary antibody (Molecular Probes). Images were taken with Zeiss Apotome using the same exposure time between samples.
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bioRxiv - Immunology 2019Quote: ... In some groups culture media was supplemented with recombinant osteopontin (2 μg/ml) (R&D) or anti-CD44 (5 μg/ml) (Thermofisher; clone IM7). Cells were cultured in 5% CO2 at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... CFTR expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Synthego, Redwood City, CA, USA), recombinant 2NLS-Cas9 nuclease (20 μM, Synthego, Redwood City, CA, USA) and optiMEM (Invitrogen, Waltham, MA, USA) supplemented with Y27632 (10 μM) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Fragments were end-repaired with Enzymatics low concentration end repair mix (Y9140-LC-L) and A-tailed with Recombinant Taq (Life Technologies, 100021276). Y-Adapters were pre-annealed by incubating a MiSeq Common Adapter with Adapters (A01-A10 ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Microbiology 2023Quote: ... The sequences of all four recombinant viruses generated in this study were confirmed by sequencing with a SeqStudio Genetic Analyzer (Thermo Fisher Scientific) and an outsourced service (Fasmac).
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bioRxiv - Developmental Biology 2023Quote: ... both diluted 1:50 in DMEM/F-12 or with 20 ug/mL recombinant truncated vitronectin (VTN-N) (Thermo Fischer Scientific, A31804) diluted in DMEM/F-12 (Gibco, 21331-020) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...