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Citations for Thermo Fisher :
601 - 650 of 7068 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The HEK293S-GnTi− cells were cultured in Freestyle 293 medium (Thermo Fisher Scientific) supplemented with 5% CO2 at 37 ° C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and HCT116 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, 11995065) with 10% FBS (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa and HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 5 - 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T and HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) which was supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2022Quote: HEK293 FT cells were transfected with the BioID2 constructs using Lipofectamine 2000 (Invitrogen) in Opti-MEM ...
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bioRxiv - Immunology 2022Quote: ... HEK293 and MC38 cells were incubated in DMEM (Meilunbio) with 10% FBS (Gibco). THP1-dual cells were incubated in RPMI-1640 (Meilunbio ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were transfected using Lipofactamine 2000 according to the manufacturer’s instructions (Invitrogen).
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... HEK293 Phoenix cells were transfected with the plasmid using Lipofectamine 3000 (ThermoFisher Scientific). The transfected cells were incubated at 37°C for 5 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids (3ug per well) were transfected into HEK293 cells using lipofectamine 2000 (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293S MGAT−/− cells were maintained in a 1:1 ratio of Freestyle (ThermoFisher) and Ex-Cell (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Cell Biology 2023Quote: Hela and HEK293 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 and HEK293T cells were cultured in DMEM containing Glutamax (Thermo Fisher Scientific) and K562 cells were cultured in RPMI 1640 containing Glutamax (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco), HCT116 cells were cultured in McCoy’s 5A modified Medium ...
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bioRxiv - Immunology 2022Quote: ... To produce the HEK293 Flp-In T-REx cells (R78007, Thermo Fisher Scientific) cell line with conditional knock-down of CNOT1 we designed a tri-miRNA construct (listed below ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2G plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 or U2OS cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific L3000015) according to the manufacturer’s instructions ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Cell Biology 2023Quote: ... and suspended in serum free medium HEK293 SFM II (Gibco, catalog #: 11686-029), supplemented with 25 mM HEPES (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-T cells were seeded in 10-tray Cell Factories (Thermo Fisher Scientific) 24 hours before transfection ...
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bioRxiv - Molecular Biology 2023Quote: For HEK293T (ATCC CRL-3216) and HEK293/Flp-In/T-Rex (Invitrogen R71007) cell lines ...
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bioRxiv - Biophysics 2024Quote: ... HEK293S GnTi- cells used for protein expression were cultured in Freestyle 293 (GIBCO) supplemented with 2% FBS and 1% Antibiotic-Antimycotic.
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bioRxiv - Biochemistry 2024Quote: HEK293-TLR3hi cells were cultured in DMEM with 4 mM L-glutamine (ThermoFisher), 10% FBS (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: Adherent HEK293S GnTI- cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Biophysics 2021Quote: The cDNA segment encoding the C1B domain of protein kinase C (PKC) δ isoenzyme from Rattus norvegicus (amino acids 229-281) was sub-cloned into pET SUMO expression vector (Invitrogen). The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... subtilis 168 as the template and appropriate nucleotides that attached BsaI and XhoI restriction sites to the fragments and cloned between the BsaI and XhoI sites of the expression vector pET-SUMO (Invitrogen, Germany). The resulting plasmid was pGP2972 ...
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bioRxiv - Molecular Biology 2021Quote: ... PICS-1EBM (residues 180-200) and a TOST-1 fragment (residues 34-54) were cloned into pET28-SUMO vector (Invitrogen™).
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bioRxiv - Microbiology 2024Quote: ... subtilis 168 as the template and appropriate nucleotides that attached BsaI and XhoI restriction sites to the fragments and cloned between the BsaI and XhoI sites of the expression vector pET-SUMO (Invitrogen, Germany). The resulting plasmid was pGP3630 ...
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bioRxiv - Bioengineering 2023Quote: ... mCXCL10-LPETG SUMO fusion proteins were purified by Ni-NTA pulldown and then treated overnight with Endotoxin Removal columns (Thermofisher, 88274). Cleavage of mCXCL10-LPETG from the greater SUMO fusion proteins was carried out via ULP1 digestion (Thermofisher ...
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bioRxiv - Biophysics 2023Quote: ... The His14-SUMO tag was separated from cys-ubiquitin by passing the dialyzed eluate over HisPur Ni-NTA Resin (Thermo Scientific). For labeling ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa S3 cervical adenocarcinoma cells and HeLa 6xHis-SUMO cells (kind gift from Ronald T. Hay, University of Dundee, UK) were cultured in DMEM medium (Gibco, Life Technologies) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... we cloned the region of the rocR gene that corresponds to amino acids 141 to 400 into pET-SUMO (Invitrogen, Germany). For this purpose ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
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bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...