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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Microdrives were painted with Vybrant DiI labeling solution (Invitrogen, Waltham, MA) prior to implantation to verify recording position post hoc.
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bioRxiv - Cell Biology 2024Quote: ... This was followed by labeling with Lysotracker Deep Red (ThermoFisher Scientific) for 15 min ...
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bioRxiv - Genomics 2024Quote: ... Quantitative PCR analyses were performed with SYBR green labeling (Thermo Scientific Maxima SYBR Green/ROX qPCR Master Mix (2X) ...
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bioRxiv - Neuroscience 2024Quote: ... Cell dye (Vybrant™ Dil Cell Labeling Solution, Thermo Fisher Scientific) was applied to the tips of the guide cannulae (to aid with histological identification of tip location) ...
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bioRxiv - Neuroscience 2021Quote: ... tertiary processing was used to enhance the strength of the punctate labeling in which a biotinylated secondary antibody (1:200) was followed by fluorescently conjugated streptavidin (SA-Alexa488, Invitrogen, 1-2 µg/ml).
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bioRxiv - Neuroscience 2021Quote: ... the averaged intensities of bands measured for each mice/protein were normalized to the average intensity of the lanes with total protein of the corresponding samples using the No-Stain labeling kit (A44449, Life Technologies, Carlsbad, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The probe was biotin labeled at the 3’ end using a Pierce™ Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific Inc.). Seven micrograms of nuclear protein were added to a binding reaction mixture containing 2µl 10X binding buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The purified protein was fluorescently labeled with Alex Fluor™ 568 and 647 protein-labeling kits (Thermo Fisher Scientific Cat# A10238 and A30009) to produce hmAb-AF568 and hmAb-AF647 following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... The full procedure for preparing miRNA for hybridization was performed using the FlashTagTM Biotin HSR RNA Labeling Kit (ThermoFisher Scientific, Waltham, MA, USA). 150 ng of previously isolated miRNA was subjected to the poly(A ...
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bioRxiv - Neuroscience 2019Quote: ... HPLC-purified dipeptide repeats were purchased from Pepscan and were either labelled with a single Cy5 dye at the N-terminus during synthesis or were labelled non-site-specifically in-house using Alexa488-Fluor® Protein Labeling Kit (Thermo Fisher Scientific) using published methods (94).
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bioRxiv - Genomics 2021Quote: ... sense ssDNA suitable for labeling was generated from total RNA with the GeneChip WT Plus Reagent Kit from Affymetrix (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... sera were harvested and IgGs were purified on protein A and labeled with Alexa Fluor™ 647 using the protein labeling kit (Thermo Fisher Scientific) as recommended by the manufacturer.
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bioRxiv - Developmental Biology 2020Quote: ... Purified cDNA was used for the in vitro synthesis of biotin labeled cRNA using the GeneChip IVT Labeling Kit (Affymetrix, Santa Clara, CA). cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was also analyzed using the carboxyfluorescein diacetate succinimidyl ester labeling method with the CellTrace™ CFSE Cell Proliferation Kit (Invitrogen, CA, USA) in cells synchronized by gradual serum deprivation following the protocol described by Lauand and collaborators 23 ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit ...
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bioRxiv - Neuroscience 2022Quote: ... the averaged intensities of the bands measured for each patient/protein were normalized to the average intensity of the lanes with the total protein of the corresponding samples using the No-Stain labeling kit (A44449, Life Technologies, Carlsbad, USA) using the fluorescent channel of the chemidocumenter.
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bioRxiv - Neuroscience 2022Quote: A total amount of 250 nanogram of total RNA from each sample was processed with the FlashTag Biotin HSR RNA Labeling kit (Thermofisher, Waltham, MA, USA) and subsequently hybridized onto the GeneChip miRNA 4.1 Arrays (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... Fractured and intact contra lateral femur sections were stained for EdU labeling using the Click-iT® EdU Alexa Fluor® 488 Imaging Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic assays: Apoptotic cells were identified by labeling 3’ ends of DNA using a BrdU apoptosis kit (Fisher Scientific cat. 88-6671-88) and anti-BRDU antibodies ...
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bioRxiv - Microbiology 2024Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit.
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bioRxiv - Biophysics 2021Quote: ... Actin labeling was performed using AlexaFluor 488- or AlexaFluor 546-phalloidin (Invitrogen), diluted together with the secondary antibody in blocking/dilution buffer to a concentration of 165 nM ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling with WGA-555 (1:200; Thermo Fisher Scientific, Walham, MA; #W32464) was performed prior to antigen retrieval ...
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bioRxiv - Immunology 2021Quote: ... mixed with 0.1 mg of TMT10-plex labeling reagents (Thermo Fisher Scientific) dissolved in 5 μL acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary labeling was performed using goat anti-rabbit (Alexa Fluor 350; ThermoFisher) and donkey anti-mouse (Alexa Fluor 647 ...
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bioRxiv - Biochemistry 2020Quote: ... the labeling was done in warm Hank’s Balanced Salt Solution (HBSS, ThermoFisher). Cytosolic translation was stopped using harringtonine (200μM ...
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bioRxiv - Cell Biology 2021Quote: For live cell labeling of lipids with NileRed (Thermo Fisher Scientific, USA), RPE organoids were incubated in a non-treated cell culture plate for 25 min at 37 °C in BRDM containing 1 μM NileRed.
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bioRxiv - Immunology 2022Quote: Hemocytes were labeled with the Vybrant CM-DiI Cell-Labeling Solution (Invitrogen) as we described (5) ...
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bioRxiv - Neuroscience 2023Quote: ... Post-embedding immunogold labeling was performed with anti-GFP (Invitrogen, #A-11120) and 9–11 nm colloidal gold conjugated to goat anti mouse IgG secondary antibodies (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: For ER labeling we used ERTrackerGreen (BODIPY FL Glibenclamide, ThermoFisher Scientific, # E34251) according to the manufacturer’s protocol (#MP 12353) ...
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bioRxiv - Neuroscience 2023Quote: ... and labeling live cells (CellTrace calcein green; ThermoFisher Scientific, Waltham, MA, USA). All antibodies were diluted in Neurobasal-A containing 2% B27 (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... 5 μL of Vybrant™ DiO Cell-Labeling Solution (V22886, Invitrogen™) was added to the NHLF suspension ...
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bioRxiv - Biochemistry 2023Quote: ... The stoichiometry of labeling was assessed using a Nanodrop One (Thermo Fisher) and comparing the absorbance of total protein at 280 nm to the absorbance at the SNAP-dye wavelength.
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bioRxiv - Cell Biology 2023Quote: Intracellular calcium labeling was prepared using Fluo-4 AM (Thermo Fisher Scientific) following manufacturer-provided protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary labeling was with anti-rabbit Alexa488 (1:1,000, ThermoFisher# A-11008). Brains were then mounted with Vectashield medium (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent dyes were employed at specific concentrations for labeling: Syto9 (Invitrogen™) - 1.7 µM ...
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bioRxiv - Plant Biology 2024Quote: ... Isobaric labeling was done using 18-plex TMTpro reagents (Thermo Scientific A52047) as follows ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... NeuroTrace CM-Dil tissue-labeling paste (N22883, Thermo Fisher Scientific, MA, USA) was injected at the same stereotaxic site to confirm the ACSF and muscimol injection site.
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bioRxiv - Neuroscience 2023Quote: ... Labeling was visualized using Pierce ECL Western Blotting Substrate (Thermo Scientific; 32209) and luminescent image analyzer LAS-3000.
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bioRxiv - Developmental Biology 2020Quote: ... The target oligonucleotide was 3’ end labeled with biotin using a Biotin 3’ End DNA labeling kit (Thermo Fisher Scientific, Waltham, MA, USA, #89818). The oligonucleotides used as probes or competitors in gel shift assays were end-labeled at their 3’ ends with biotin ...
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bioRxiv - Neuroscience 2019Quote: ... were directly labeled with fluorochromes following standard protocols of different labeling kits (art. no. A20181, A20183, A20186, 21335 for labeling with Alexa 488, Alexa 546, Alexa 647, and biotin, respectively; Thermo Fisher Scientific, Waltham, USA). Each protocol started with an indirect immunolabeling using unlabeled primary antibodies raised in rabbit/mouse using their appropriate secondary antibodies (with different conjugates ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were azide-labeled using Click-iT O-GlcNAc Enzymatic Labeling System and reacted with biotin alkyne using Click-IT Biotin Protein Analysis Detection Kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... the probe mixture was heated to 95°C for 5 min to denature it and then incubated for 30 min at 80°C with 10 µL of the red emission dye Alexa594 (Ulysis™ Alexa Fluor® 594 Nucleic Acid Labeling Kit, Thermofisher, cat.no: U21654). The unbound Alexa594 was removed using chromatography columns (Micro Bio-spin chromatography columns P-30 ...
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bioRxiv - Synthetic Biology 2020Quote: Ten percent of the final RNA amount was used for chemical labelling with Cy3 fluorescent dye using Arcturus Turbo Labeling Cy3 Kit (ThermoFisher, Applied Biosystems, Cat No. KIT0609). The chemical labelling procedure and subsequent removal of free Cy3 dye was performed following manufacturer’s protocol with 15 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the selected targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments against the mRNA of the cluster HVO_B0192-HVO_B0193 and against the mRNA of HVO_0739.
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bioRxiv - Microbiology 2021Quote: ... For hybridisation experiments radioactively labeled PCR probes against the desired targets were generated using DECAprime II DNA labeling kit and [α-32P]dCTP (Life Technologies, Thermo Fisher Scientific). Membranes were subsequently incubated with the labeled PCR fragments.
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bioRxiv - Cell Biology 2023Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was performed to assess cell apoptosis using commercial apoptosis detection kit (Thermo Fisher Scientific, United States). All procedures were done according to the directions of the manufacturers ...
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bioRxiv - Biochemistry 2023Quote: ... with stable isotope labeling performed according to manufacturer instructions using the Thermo Scientific™ SILAC™ Protein Quantitation Kit (Catalog A33972, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Systems Biology 2024Quote: TMT labeling was performed according to our previously established dry TMT labeling workflow using an 11-plex TMT Mass Tagging Kit (Thermo Fisher Scientific, lot XB323735) 13 ...