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Citations for Thermo Fisher :
601 - 650 of 8005 citations for Recombinant Human TEK His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
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bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
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bioRxiv - Cell Biology 2019Quote: ... 0.3μg of GFP-tagged construct DNA were mixed with 0.7μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1μg of GFP-tagged construct DNA were mixed with 0.2μg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of 6xHis-tagged proteins and Strep-tagged proteins was tested with 6xHIS TagAntibody HIS.H8 (Invitrogen Co.) and StrepMAB-Classic ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg of GFP-tagged construct DNA were mixed with 0.5µg of mchr-tagged construct DNA and diluted in Optimem (ThermoFisher) without serum or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then incubated with fluorescently tagged secondary antibodies and/or fluorescently tagged Phalloidin (ThermoFisher Scientific, Waltham, MA) diluted in the blocking buffer at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescently tagged secondary antibodies were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-tagged phalloidin (ThermoFisher, 400x stock) was added at 4x working concentration and incubated for 2 days ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor-tagged secondary antibodies (Invitrogen) were matched to the primaries and incubated for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... GST-NtRac5 were immobilized on glutathione-agarose (Thermo Fisher Scientific) and incubated with purified recombinant MBP-AtPIP5K2 protein for 60 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... the GST-RNF38RING was incubated with glutathione agarose (Thermo Fisher). The UBC13~Ub conjugate was mixed with GST-RNF38RING (60 μM ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific, #10368552), 5 mM DTT ...
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bioRxiv - Immunology 2019Quote: ... and anti-glutathione-S-transferase (GST) antibody (ThermoFisher 71-7500). Antibody-bound complexes were collected ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-HA.11 antibody was purchased from Biolegend and antibodies against V5 and GST tags were from ThermoFisher.
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-GST (1:500, Thermo Fisher, MA4-004). For visualisation ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary infrared anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-GST (1:1000) (cat#8-326, Thermofisher scientific) antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary infrared anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Biophysics 2024Quote: ... or GST using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001), and after 48h ...
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bioRxiv - Cell Biology 2020Quote: ... Chamber slides (Lab-Tek, Nunc, Thermo-Fisher Scientific, Rochester, NY) were coated with 50 μg/ml fibrinogen and blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... 125 μl in 8-well lab-Tek chambered coverglass (Nunc); or 20 μl in 384-well glass bottom SensoPlate (Greiner) ...
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bioRxiv - Neuroscience 2021Quote: ... on Nunc Lab-Tek II Chamber Coverglass (ThermoFisher Scientific, 155409PK) (plated at 30,000 neurons/well for confocal microscopy) ...
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bioRxiv - Molecular Biology 2021Quote: Cells were grown in Lab Tek II chambers (Thermo Fisher), fixed with 4 % paraformaldehyde/1xPBS for 10 min at RT and solubilized with 0.1% Triton X-100 /1xPBS before staining with antibodies against LC3A/B (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were seeded into Lab-Tek II Chamber Slide (Thermofisher) or Number 1 cover slips in a 24-well plate for 24 to 48 h ...
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bioRxiv - Cancer Biology 2019Quote: Cells were grown in chambered Lab-Tek coverglass wells (Nunc), washed with DPBS ...
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bioRxiv - Bioengineering 2019Quote: Nunc Lab-Tek II 4-well chamber slides (Thermo Fisher) were pre-coated with Poly-D-Lysine (Trevigen ...
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bioRxiv - Cancer Biology 2021Quote: ... Lab-Tek 2-well glass chamber slides (Thermo Fisher Scientific) were coated with 50 μg/ml of collagen type I (Gibco ...
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bioRxiv - Cell Biology 2021Quote: MSCs were seeded on Lab-Tek Chamber slides (Thermo Fisher) at 9×103 cells/cm2 ...
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bioRxiv - Molecular Biology 2020Quote: Lab-Tek II 8-well chamber slides (ThermoFisher Scientific, USA) were coated with 300 μl 100 μg/ml poly-L-lysine per well for 1.5 hours at 37°C and then washed three times with ddH2O ...
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bioRxiv - Neuroscience 2021Quote: ... Chamber slides (Nunc™ Lab-Tek™, Thermo Fisher Scientific) were used for immunocytochemistry and FluoroDish (FD35-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Chamber slides (Nunc™ Lab-Tek™, Thermo Fisher Scientific) were used for immunocytochemistry and FluoroDish (FD35-100 ...
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bioRxiv - Cell Biology 2022Quote: ... chambers from Lab-Tek Chamber slides (Nunc, Rochester, NY, USA) were removed and placed over the MAS slide ...
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bioRxiv - Plant Biology 2022Quote: ... a Lab-Tek Chambered Coverglass w/cvr (Thermo Fisher Scientific) was used as described previously (Mabuchi et al. ...
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bioRxiv - Molecular Biology 2023Quote: Samples were imaged in NUNC chambers (Lab-Tek, Thermo Scientific) that were pre-coated with a solution of 0.01% Tween 20 (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cells were seeded into Lab-Tek Chambered Coverglass (NUNC, 155409) and treated with vehicle or indicated metabolic precursors for 48 h ...
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bioRxiv - Cell Biology 2023Quote: ... or 8-well Nunc Lab-Tek slides (Thermo Scientific, #125658) were coated with Poly-L-Lysine 0.01% (v/v ...
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bioRxiv - Physiology 2023Quote: ... placed in a Tissue-Tek Cryomold (Fisher Scientific, Pittsburg, PA), snap frozen ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).