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Citations for Thermo Fisher :
551 - 600 of 8005 citations for Recombinant Human TEK His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Cell Biology 2024Quote: IMR90 fibroblasts were differentiated to myofibroblasts with 5ng/mL Recombinant Human Transforming Growth Factor -β1 (TGF-β1) (Invitrogen, #PHG9204) for 48h in serum free media ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Purification was carried on GST affinity resin (Thermo Scientific) and reduced glutathione was used for protein release ...
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bioRxiv - Biophysics 2022Quote: ... Anti-GST Alexa488-conjugated antibody was purchased from ThermoFisher Scientific (Catalog number ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 8-well Lab-Tek II chambers (Thermo Fisher Scientific). To fix cells ...
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bioRxiv - Biochemistry 2020Quote: ... or Nunc Lab-Tek II Chambered Coverglass (Nalgene Nunc/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... into Lab-Tek on glass coverslip (155411-Thermo Scientific) were acquired using a 100x Plan Apochromat oil immersion NA1.4 objective on a spinning disk system Axio Observer Z1 (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... Lab-Tek II (Thermo Fisher Scientific Japan, Yokohama, Japan); cells used for references for chromatic correction were also cultured in a chamber of the same coverglass ...
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bioRxiv - Cell Biology 2020Quote: ... followed by embedding with Tissue-Tek (Thermo Fisher, 12351753) for frozen sections.
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bioRxiv - Cell Biology 2019Quote: ... cells were seeded on Lab-Tek Chambered Coverglass (ThermoFisher). Fluorescent images were captured on a Nikon A2 confocal microscope ...
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bioRxiv - Biophysics 2022Quote: In a Lab-Tek II chambered coverglass (Fisher Scientific), 150 μL of GUVs were mixed with 150 μL of isosmotic buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... coverslips (Nunc Lab-Tek Chambered Coverglass, Thermo Fisher Scientific). Background samples were prepared similarly but without protein ...
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bioRxiv - Immunology 2023Quote: ... The usage of 16-well Lab-Tek Chambers (Nunc) enabled us to record up to 12 different conditions (i.e ...
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bioRxiv - Biophysics 2023Quote: ... Nunc Lab-Tek Chambered coverglass (Thermo Fisher Scientific, USA), NECA (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... Lab-Tek Chambered Coverglass w/cvr (Thermo Fisher Scientific) was used ...
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bioRxiv - Cell Biology 2024Quote: XB2 keratinocytes were plated on Nunc Lab-Teks (Thermofisher), at a confluency of 1.5×104 cells/well ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
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bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
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bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
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bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
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bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
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bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...