Labshake search
Citations for Thermo Fisher :
601 - 650 of 6237 citations for Malate dehydrogenase cytoplasmic MDH1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
-
bioRxiv - Biochemistry 2022Quote: The remainder of the cytoplasmic fraction was added to 5 µL of washed Pierce Protein G agarose beads (Thermo Fisher Scientific), and the mixture was rotated 1-2 hours at 4°C to pre-clear ...
-
bioRxiv - Microbiology 2022Quote: Differentiated LT-treated and control enteroid monolayers were lysed to using NE-PER™ nuclear and cytoplasmic extraction reagent (Thermo Scientific). Cell membrane pellets were solubilized in PBS containing 1% Triton X-100 supplemented with protease inhibitor cocktail (Pierce™ protease inhibitor mini ...
-
bioRxiv - Microbiology 2022Quote: ... MYXV infection or Selinexor + MYXV and nuclear and cytosolic fractions were prepared using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific). Purity of the fractions was confirmed by western blot analysis for tubulin (cytoplasmic ...
-
bioRxiv - Systems Biology 2022Quote: The whole kidney was homogenized and nuclear fraction was extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific, # 78833), following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2023Quote: ... directed against the cytoplasmic domain of E-cad and revealed using an anti-mouse IgG (H+L) secondary antibody (Alexa Fluor 555) (Life Technologies). The 4’,6’-diamino-2-fenil-indol (DAPI ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclear and cytosolic protein fractions from muscle biopsies were prepared using NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Catalog. No 78835; ThermoFisher) according to the manufacturer instructions with the help of Dounce homogenizer.
-
bioRxiv - Neuroscience 2022Quote: Cortex from 3-4 months mice brains were dissected and homogenized (pool of three animals) in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Immunology 2023Quote: ... Cytoplasmic microinjection was performed in C57BL/6J zygotes using a mix of 25 ng/µl Cas9 mRNA (Invitrogen, Carlsbad, CA, US), 15 ng/µl of each sgRNA (2 guides were used ...
-
bioRxiv - Genetics 2023Quote: ... Preparation of nuclear and cytoplasmic fractions was performed using the NE-PER Nuclear Protein Extraction Kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s included protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific). Equal amounts of protein lysates (20 µg ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... conjugated to mOxGFP and deficient of cytoplasmic tail (ΔCT) was maintained in DMEM/F12 media supplemented with 5% horse serum (16050122; Thermo Fisher), 20 ng/mL EGF (Peprotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were harvested after 48 hr of transfection and fractionated according to the manufacturer’s instructions using NE-PER Nuclear and Cytoplasmic Extraction Reagents (#78833, Thermo Fisher Scientific). Protease inhibitor cocktail (P8340 ...
-
bioRxiv - Molecular Biology 2024Quote: ... or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific). Equal amounts of protein lysates (20 µg ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 μg monoclonal anti-Syp antibody directed against a cytoplasmic epitope (cat no 101011, synaptic systems, Germany) or control mouse IgG (10400C, Thermofisher Scientific) was used to incubate with 20-30 μl beads for 30 mins at RT in 2% BSA dissolved in IP buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
-
bioRxiv - Molecular Biology 2021Quote: ... and Western blotting was performed with anti-His (ThermoFisher MA1-135), anti-HA (Roche 11583816001) ...
-
bioRxiv - Developmental Biology 2020Quote: ... The Ion Hi-Q Sequencing 200 Kit (Thermo Fisher Scientific, UK) and the Ion Proton platform were used for analysis ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
-
bioRxiv - Cell Biology 2021Quote: ... pRSET-His-GFP-3C-Survivin (pRSET vector from Thermo Fisher Scientific), pMCNcs-INCENP1-58 and pETM-His-TEV-Borealin10-280 or pETM-His-TEV-Borealin10-221 (pETM vector ...
-
bioRxiv - Biophysics 2021Quote: ... Clarified lysate was bound to His-Pur Cobalt resin (Thermo Scientific) for 2 hours with rocking at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... The Ion PGMTM Hi-QTM View OT2 kit (Life Technologies, USA) and Ion OneTouch DL instrument (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
-
bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
-
bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
-
bioRxiv - Zoology 2019Quote: ... Blots were probed with rabbit anti-His antibody (Thermo Fisher Scientific) at 1 ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
-
bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
-
bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
-
bioRxiv - Biochemistry 2019Quote: ... and His-BMAL1 FL were co-infected into monolayer HighFive (Invitrogen) insect cells for protein expression ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
-
bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
-
bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
-
bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
-
bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
-
bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
-
bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
-
bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
-
bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
-
bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
-
bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.