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Citations for Thermo Fisher :
451 - 500 of 6237 citations for Malate dehydrogenase cytoplasmic MDH1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or HCoV-OC43 N-His (Sino Biological # 40643-V07E ...
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bioRxiv - Cell Biology 2022Quote: ... were cloned in pcDNA3.1(-) Myc/His vector (ThermoFisher Scientific). Ligated plasmids were transformed in chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
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bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Physiology 2019Quote: ... and 1 µL of either dystrophin (Dmd) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe/primer mix (Applied Biosystems; Mm00464475_m1 and Mm99999915_g1 ...
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bioRxiv - Microbiology 2020Quote: Cell cytotoxicity was determined with CyQUANT Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit (Invitrogen/Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Cell cytotoxicity was determined with CyQUANT Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit (Invitrogen/Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell damage was quantified by assaying the release of lactate dehydrogenase (CyQUANT™ LDH Cytotoxicity Assay, Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... Lactate dehydrogenase (LDH) release was quantified from these supernatants using the CyQuant™ LDH Cytotoxicity Assay Kit (Invitrogen) following provided protocols from the manufacturer ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs02786624_g1) using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific Inc.). Relative mRNA levels of each gene were normalized to GAPDH levels as a housekeeping control.
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bioRxiv - Molecular Biology 2020Quote: ... Two rounds of polyA selection from total cytoplasmic lysate and polysome fractions were performed using oligo (dT) Dynabeads (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Nuclear extracts were prepared from K562 cells using NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher, Waltham, MA) supplemented with HALT Protease Inhibitor Cocktail and PMSF (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... PBS was aspirated and cells were taken forward for nuclear/cytoplasmic fractionation using NE-PER kit (ThermoFisher Scientific, 78835) following manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... A total of 1 mg cytoplasmic protein was incubated with 100 μL Dynabeads M-280 streptavidin (Thermo Fisher Scientific) conjugated with 5′-biotinylated siRNA or 100 bp linearized DNA ...
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bioRxiv - Systems Biology 2021Quote: ... cells were co-transfected with plasmids coding cytoplasmic Cerulean and D4H-mVenus (0.25 μg/well each) using Lipofectamine 2000 (0.75 μl/well, Invitrogen, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... Subcellular protein fractionations were performed using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, 78833), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Kit (78833; Thermo Scientific, Rockford, IL, USA). The protein concentrations of both types of extracts were measured using the BCA Protein Assay Kit (23227 ...
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bioRxiv - Cancer Biology 2020Quote: ... and H524 cell lines were subjected to subcellular fractionation using NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatants were monitored over time for cytoplasmic leakage via OD260 measurements using a spectrophotometer (Biomate 3S, Thermo Scientific). For this ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear and cytosolic extracts were prepared using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents kit (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... fractionation of nuclear and cytoplasmic fractions was carried out using the Ambion® PARIS™ kit (Invitrogen, Cat # AM1921). Total RNA was isolated with TRIzol™ (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: Cytoplasmic and nuclear RNAs from DRG neurons were isolated using the PARIS™ Kit (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... we prepared nuclear and cytoplasmic fractions from untreated cells using a PARIS™ kit (Thermo Fisher Scientific, cat# AM1921).