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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.5% Albumax II (Invitrogen), 0.25% sodium bicarbonate ...
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bioRxiv - Bioengineering 2022Quote: ... collagen II (MA512789; Thermofisher), or collagen X (14-9771-82 ...
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bioRxiv - Microbiology 2022Quote: ... Cellfectin II Reagent (Invitrogen) was used for DNA transfection at a cell density of 8 × 105 per well ...
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bioRxiv - Molecular Biology 2023Quote: ... and superscript II (Invitrogen) based on the instruction given by the manufacturers ...
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bioRxiv - Molecular Biology 2022Quote: ... SuperScript II (Thermo Fisher), SuperScript III (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Albumax II (Gibco)) at 4% haematocrit in washed whole blood (National Blood and Transfusion Service or Centro de Transfusiones de la Comunidad de Madrid CTCM ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Albumax II (GIBCO), 25 mM HEPES (GIBCO) ...
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bioRxiv - Biochemistry 2023Quote: ... SF900-II media (ThermoFisher) was supplemented with 5% FBS (HyClone ...
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bioRxiv - Genetics 2022Quote: ... LR clonase II (ThermoFisher) enzyme was then used to shuttle sequence verified reference and variant ORFs into the p.UASg-HA.attB destination vector ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% Albumax II (Gibco), 0.36 mM hypoxanthine (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% AlbuMAX II (GIBCO).
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Albumax II (Gibco)) under a 1% O2 ...
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bioRxiv - Physiology 2024Quote: ... SuperScript II RT (Invitrogen) was added to each reaction and incubated at 42°C for 50 minutes prior to enzyme inactivation by heating at 70°C for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Batch cultivation was performed in 250 mL shake flasks containing 60-80 mL CD-CHO medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 8 mM L-glutamine (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection were performed (∼1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2021Quote: ... were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Expression SΔTM trimers was performed by transfection of ExpiCHO-S™ cells with the plasmids described above using the ExpiFectamine™ CHO transfection kit (Gibco™ ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine™ CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... The plasmid expression plasmid carrying each SARS-CoV-2-associated protein was transiently transfected into ExpiCHO-S cells by using the ExpiFectamine™ CHO transfection kit according to the manufacturer’s instruction (Thermo Scientific, A29129). After transfection ...
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bioRxiv - Microbiology 2021Quote: ... IgG or Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... GenBank accession number NM_004519) was generated using Flp-In™-CHO cells according to the supplier’s protocol (Thermo Fisher Scientific, Waltham, USA). CHO-Q3 stable cells were maintained under dual selection with Zeocin (100 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... To identify these target cells during downstream analysis CHO DP-12 cells were first stained with CellTracker™ Blue CMAC Dye (Thermo Fisher). 85 µm nanovials containing RGD and cells were seeded into a 12-well plate as described above and then incubated at 37°C for 2 hours to allow cells to adhere to the particles ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Expression plasmids encoding the heavy and light chains of the COVA1-16 Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection was performed (∼1 mL per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2020Quote: ... microscale transfection were performed (~1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2022Quote: Recombinant human β2m-MR1-K43A-Fc was produced in CHO-K1 cells as previously described (10) and 4 µg/mL were coated onto 96 wells plates (Nunc, Cat#439454) for 18 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... These confluent CHO cells were transfected with 700 ng pUNO1-Mincle or pUNO1-DC-SIGN using TurboFect Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The AF-03 and AF03-NL plasmid were transfected into ExpiCHO-S cells using the ExpiFectamine™ CHO Transfection Kit (Gibco, A29129) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ T cells were co-cultured with CHO cells for 16 hours in the presence of T cell activation using anti-CD3/CD28 Dynabeads™ (ThermoFisher Scientific) at an estimated 1:1 bead-to-cell ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... microscale transfection was performed (∼1 ml per antibody) with CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA (Mouse IFN gamma Uncoated ELISA (Invitrogen, #88-7314); Mouse TNF alpha Uncoated ELISA (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... vaccinated-challenged and unvaccinated-challenged animal were measured by Bovine IFN-ϒ-ELISA kit (Invitrogen, Frederick, USA) as per the instructions of the manufacturer.
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bioRxiv - Immunology 2023Quote: ... IL-4 and IFN-ᵧ in supernatant was measured according to the manufacturer’s protocol (all Life Technologies).
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bioRxiv - Genetics 2019Quote: ... and then reverse-transcribed into cDNA using Superscript II or II (Thermo Fisher) following the standard protocol ...
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bioRxiv - Bioengineering 2021Quote: ... platelet-derived growth factor receptor A (PDGFRA) and type I collagen (COL1A1) (Thermo Fisher, Waltham, Massachusetts). Probe references ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells expressing ACE2 receptors were suspended using TrypLE Select Enzyme solution (Thermo Fisher Scientific) and immediately added to all wells (10,000 cells in 100 μl of growth medium per well) ...
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bioRxiv - Neuroscience 2021Quote: ... surface receptors were labeled with Pierce™ Premium Grade Sulfo NHS-SS-Biotin (Thermofisher, Waltham, USA) and purified using Streptavidin High Performance Spintrap™ (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Receptor and chemokine baculovirus stocks were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... western blots were performed to normalize for receptor quantity using monoclonal anti-HA antibody (Thermo Scientific). Reactions were started by adding 150μL partially purified receptor samples to 300μL of reaction mixture and incubating on ice for 1 hour ...