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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... or RNA polymerase II (POL-II) (Invitrogen # MA5-23510, 1:500) for 1 h at room temperature [50 ...
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bioRxiv - Biochemistry 2019Quote: ... supplemented with 1% Protein Free Hybridoma Medium II (PFHM II, GIBCO) (basal medium ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... the causal relation that a target gene is up- or downregulated depending on the transcription complex being active or inactive and (ii) the causal relation that a signal from a gene expression microarray (e.g. Affymetrix Human Genome U133 Plus 2.0) probeset is high or low depending on the target gene being up or down ...
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bioRxiv - Microbiology 2020Quote: ... plasmids encoding targeted proteins were transiently transfected into ExpiCHO-S cells by using ExpiFectamine™ CHO transfection kit (Thermo Scientific, A29129). Transfected cells were cultured in stackable CO2 incubator shaker (Kühner AG ...
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bioRxiv - Microbiology 2021Quote: ... 100 µg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Bioengineering 2021Quote: CHO cells and CHO EVs for SEM were fixed in 4% EM grade glutaraldehyde overnight on poly-L-lysine coated glass coverslips (Fisher Scientific). Samples were processed for SEM imaging by treating the samples to 1% osmium tetroxide for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... CHO cells stably expressing LI-cadherin-GFP were established using Flp-In™-CHO Cell Line following the manufacturer’s protocol (ThermoFisher Scientific). Cloning was performed by the limiting dilution-culture method ...
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bioRxiv - Microbiology 2019Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Biochemistry 2021Quote: ... One ampule of cells from a working cell bank was thawed in CD-CHO basal medium (Thermo Fisher Scientific, Waltham, MA), along with 0.5 % anti-clumping agent (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ExpiFectamine CHO Enhancer (6 μl per ml of culture) and ExpiCHO Feed (240 μl per ml of culture) (Thermo Fisher Scientific) were added 18-22 hours post-transfection and the temperature was decreased to 33 °C ...
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bioRxiv - Microbiology 2019Quote: ... EDE2 B7 (Dejnirattisai et al., 2015), CR4354 (Kaufmann et al., 2010) were expressed by transient transfection of Expi-CHO-S cells (Cat# A29129; ThermoFisher Scientific). Variable heavy and light chain sequences of the above control mAbs used for gene synthesis and cloning into expression vectors were based on PDB IDs 4UT9 ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Transfections of NIH 3T3 and CHO cells were carried out using a Neon MP-100 microporator system (Life Technologies, Carlsbad, CA). Transfection of HEK293 cells was performed using the calcium phosphate method ...
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bioRxiv - Neuroscience 2023Quote: Chinese hamster ovary (CHO) cells were maintained in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... trimer was produced and differentially purified from a CZA97.012 SOSIP.664-expressing CHO-cell line maintained in ExpiCHO Expression Medium (Thermo Fisher Scientific) (82) ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and GlyA-CHO cells (glycine auxotrophic for growth and a gift from Dr. P. J. Stover, Cornell University) using Lipofectamine 2000 (Invitrogen # 11668019). Briefly ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Immunology 2019Quote: ... The probes specific for IFN or inflammatory cytokines were purchased from Applied Biosystems (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... IFNγ levels were measured in the supernatant of the WBA using IFN gamma ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... followed by incubation with primary antibodies to IFN-α (1:500, Thermo Fisher Scientific, MA5-37518), IFN-β (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... IFN-gamma protein expression was analysed using Luminex xMAP technology on LuminexTM MagPix200 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... supernatant was collected and IFN-ɑ measured by ELISA according to manufacturer’s instructions (Invitrogen; catalog # BMS6027)
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were incubated with Polyclonal Glucocorticoid Receptor (2 ug; clone PA1-511A, Thermo Fisher Scientific) followed by Donkey anti-Rabbit IgG AF647 (0.5ug ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
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bioRxiv - Cell Biology 2019Quote: We used the following mouse monoclonal antibodies against: transferin receptor (13-6800, Invitrogen, 1/200); CD63 [53] ...
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bioRxiv - Neuroscience 2020Quote: ... IgG1 anti-transferrin receptor (TfR, clone H68.4, 1:500, ThermoFisher Scientific, catalog no. 13-6800), IgG1 anti-GAPDH (1:10,000 ...
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bioRxiv - Physiology 2023Quote: ... The anti-transferrin receptor mouse monoclonal antibody (TransR, clone H68.4, Thermo Fisher Scientific, 1:1000) and the anti-alpha 1 Na+/K+-ATPase mouse monoclonal antibody (Na+/K+-ATPase α1 ...
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bioRxiv - Molecular Biology 2023Quote: ... recombinant GR-LBD-GST (A15668) and Nuclear Receptor Buffer F (PV4547) were all from Invitrogen.
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... ii) Phusion Hot Start II High-Fidelity PCR Master Mix (Thermo Scientific) was used for PCR amplification and ...
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bioRxiv - Molecular Biology 2023Quote: ... labeled at its 5’-end with [32P]-γ-ATP using T4 Polynucleotide kinase (Thermo Fisher) according to the supplier instructions.
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bioRxiv - Microbiology 2023Quote: Purified nucleic acids were [γ-32P]-ATP labelled with T4 polynucleotide kinase (PNK; Thermo Scientific) in an exchange-labelling reaction ...
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bioRxiv - Genomics 2020Quote: ... Super-Script II (Invitrogen) was used to synthesize cDNA from 2 μg of total RNA isolated from each tissue sampled in 70 dpc goat and 76 dpc sheep fetuses ...
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bioRxiv - Microbiology 2019Quote: ... and Albumax II (Gibco) 0.5% wt/vol ...
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bioRxiv - Cell Biology 2021Quote: ... 5% albumax II (Invitrogen) and other additives as above until parasites re-appeared47 ...
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bioRxiv - Cancer Biology 2022Quote: ... counted (Countess II, ThermoFisher) and normalized for equal cell number between samples ...
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bioRxiv - Cell Biology 2021Quote: ... using Superscript II (Invitrogen) and random hexamers (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... 0.5% Albumax II (Invitrogen), 0.25% sodium bicarbonate ...
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bioRxiv - Cell Biology 2019Quote: ... using SuperScript II (ThermoFisher) kit as suggested by the protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... LR Clonase II (Thermofisher) was used to transfer the HRV3C-TOP2B insert into pcDNA6.2/N-YFPDest to generate the YFP-TOP2B plasmid ...
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bioRxiv - Biophysics 2020Quote: ... Cellfectin II reagent (Invitrogen) was used for transfection of recombinant 5-HT3AR bacmid DNA into sub-confluent Sf9 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Dispase II (Gibco) and 30 μg/ml DNase I in PBS for 45 min at 37°C ...